Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   PCP57_RS06550 Genome accession   NZ_CP115287
Coordinates   1400625..1401248 (+) Length   207 a.a.
NCBI ID   WP_003103268.1    Uniprot ID   G3XD51
Organism   Pseudomonas aeruginosa strain F006     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1395625..1406248
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PCP57_RS06535 (PCP57_06540) - 1396312..1398783 (-) 2472 WP_039026606.1 penicillin-binding protein 1A -
  PCP57_RS06540 (PCP57_06545) pilM 1398968..1400032 (+) 1065 WP_003110888.1 type IV pilus assembly protein PilM Machinery gene
  PCP57_RS06545 (PCP57_06550) pilN 1400032..1400628 (+) 597 WP_003095839.1 type 4a pilus biogenesis protein PilN Machinery gene
  PCP57_RS06550 (PCP57_06555) pilO 1400625..1401248 (+) 624 WP_003103268.1 type 4a pilus biogenesis protein PilO Machinery gene
  PCP57_RS06555 (PCP57_06560) comL/pilP 1401245..1401769 (+) 525 WP_003095836.1 type 4a pilus biogenesis lipoprotein PilP Machinery gene
  PCP57_RS06560 (PCP57_06565) pilQ 1401823..1403967 (+) 2145 WP_083556223.1 type 4a pilus secretin PilQ Machinery gene
  PCP57_RS06565 (PCP57_06570) aroK 1403979..1404497 (+) 519 WP_003095833.1 shikimate kinase AroK -
  PCP57_RS06570 (PCP57_06575) aroB 1404547..1405653 (+) 1107 WP_033977028.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 207 a.a.        Molecular weight: 22819.26 Da        Isoelectric Point: 4.7787

>NTDB_id=701980 PCP57_RS06550 WP_003103268.1 1400625..1401248(+) (pilO) [Pseudomonas aeruginosa strain F006]
MSLASSLESLRKIDINDLDLNNIGSWPAAVKVIVCVLLTAAVLALGYNFHLSDMQAQLEQQAAEEETLKQQFSTKAFQAA
NLEAYKAQMKEMEESFGALLRQLPSDTEVPGLLEDITRTGLGSGLEFEEIKLLPEVAQQFYIELPIQISVVGGYHDLATF
VSGVSSLPRIVTLHDFEIKPVAPGSTSKLRMSILAKTYRYNDKGLKK

Nucleotide


Download         Length: 624 bp        

>NTDB_id=701980 PCP57_RS06550 WP_003103268.1 1400625..1401248(+) (pilO) [Pseudomonas aeruginosa strain F006]
ATGAGTCTGGCCAGTTCCCTGGAAAGTCTGCGCAAGATCGATATCAACGATCTCGACCTGAACAACATCGGTTCCTGGCC
GGCGGCGGTCAAGGTCATCGTCTGCGTGCTGCTGACCGCGGCGGTCCTGGCGCTGGGCTACAACTTCCATCTGAGCGACA
TGCAGGCTCAGCTCGAACAGCAGGCCGCGGAAGAGGAGACGCTCAAGCAGCAGTTCTCCACCAAGGCCTTCCAGGCCGCG
AACCTGGAAGCCTACAAGGCACAGATGAAGGAGATGGAAGAGTCCTTCGGCGCCTTGCTGCGGCAGTTGCCCAGCGACAC
CGAGGTACCTGGGCTGCTCGAGGACATCACTCGTACCGGCCTGGGCAGCGGCCTGGAGTTCGAGGAAATCAAGCTGCTTC
CCGAGGTTGCCCAGCAGTTCTACATCGAGCTGCCGATCCAGATCAGCGTGGTCGGCGGCTACCACGACCTGGCGACCTTC
GTCAGCGGCGTGTCCAGCCTGCCGAGGATCGTCACCCTGCATGACTTCGAGATCAAGCCGGTCGCGCCCGGCAGCACGTC
CAAGCTGCGCATGAGCATCCTGGCCAAGACCTATCGCTACAACGACAAGGGGCTGAAGAAATGA

Domains

Predicted by InterProScan.

Pilus assembly protein, PilO (PilO)

(58-202)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB G3XD51

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Legionella pneumophila strain ERS1305867

46.114

93.237

0.43
  pilO Acinetobacter baumannii D1279779

43.979

92.271

0.406
  comO/pilO Acinetobacter baylyi ADP1

43.455

92.271

0.401