Detailed information NTG0701906

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: NIY03_RS14245
• Genome accession: NZ_CP099855
• Coordinates: 2969011..2970129 (+)
• Length: 372 a.a.
• NCBI ID: WP_000347034.1
• Uniprot ID: A0A009HMD8
• Organism: Acinetobacter baumannii strain 17978R
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2964011..2975129

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
NIY03_RS14235 (NIY03_14235)	-	2967127..2967819 (-)	693	WP_001108509.1	YggS family pyridoxal phosphate-dependent enzyme	-
NIY03_RS14240 (NIY03_14240)	pilT	2967946..2968983 (+)	1038	WP_000355489.1	type IV pilus twitching motility protein PilT	Machinery gene
NIY03_RS14245 (NIY03_14245)	pilU	2969011..2970129 (+)	1119	WP_000347034.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
NIY03_RS14250 (NIY03_14250)	fur	2970199..2970636 (-)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
NIY03_RS14255 (NIY03_14255)	bamE	2970748..2971146 (+)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
NIY03_RS14260 (NIY03_14260)	-	2971201..2971524 (-)	324	WP_000442590.1	RnfH family protein	-
NIY03_RS14265 (NIY03_14265)	-	2971524..2972591 (-)	1068	WP_004748097.1	hypothetical protein	-
NIY03_RS14270 (NIY03_14270)	-	2972744..2973199 (-)	456	WP_000782976.1	bacteriohemerythrin	-
NIY03_RS14275 (NIY03_14275)	-	2973452..2974300 (-)	849	WP_000336554.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41891.09 Da
• Isoelectric Point: 6.6943

>NTDB_id=701906 NIY03_RS14245 WP_000347034.1 2969011..2970129(+) (pilU) [Acinetobacter baumannii strain 17978R]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRKEFADTRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=701906 NIY03_RS14245 WP_000347034.1 2969011..2970129(+) (pilU) [Acinetobacter baumannii strain 17978R]
ATGGATTTTAATGACCTACTCAACCTGATGGTTGAAAAGAAATCTTCCGATCTCTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAACAGCCTTTCTGGGGAAGTAATTGGTCAACTGTTACATTCCA
TTATGAGTGAAAAACAACGCAAAGAATTTGCAGACACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCAGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGTGGCATTATTATTTTCGTAGGTGCGACAG
GTACAGGTAAATCTACTTCATTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGCCATCTTGTATTAGCTACCATGCACGCCAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGATCGTCATAGTCAGCTTTACATGGACTTGTCACTTAACTTAAAGGCAATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAGGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCAGATGAAGGTCCTGATCAATTATCAGACGGAAAACAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (34-270)

3D structure

• AlphaFold DB: A0A009HMD8

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.661	100	0.769
pilU	Pseudomonas stutzeri DSM 10701	69.022	98.925	0.683
pilU	Vibrio cholerae strain A1552	53.243	99.462	0.53
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	41.908	93.011	0.39