Detailed information NTG0701583

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: NHZ69_RS02030
• Genome accession: NZ_CP099797
• Coordinates: 437786..438934 (+)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain PAO1-N
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 432786..443934

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
NHZ69_RS02005 (NHZ69_02005)	-	433937..434233 (-)	297	WP_031655952.1	DUF167 domain-containing protein	-
NHZ69_RS02010 (NHZ69_02010)	-	434230..434823 (-)	594	WP_003084544.1	YggT family protein	-
NHZ69_RS02015 (NHZ69_02015)	proC	434834..435655 (-)	822	WP_003114890.1	pyrroline-5-carboxylate reductase	-
NHZ69_RS02020 (NHZ69_02020)	-	435667..436359 (-)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
NHZ69_RS02025 (NHZ69_02025)	pilT	436574..437608 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
NHZ69_RS02030 (NHZ69_02030)	pilU	437786..438934 (+)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
NHZ69_RS02035 (NHZ69_02035)	czcD	438942..439841 (-)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
NHZ69_RS02040 (NHZ69_02040)	-	439995..440399 (+)	405	WP_003084560.1	NINE protein	-
NHZ69_RS02045 (NHZ69_02045)	-	440642..442015 (+)	1374	WP_003100955.1	pyridoxal-phosphate dependent enzyme	-
NHZ69_RS02050 (NHZ69_02050)	-	442012..443196 (+)	1185	WP_003100953.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=701583 NHZ69_RS02030 WP_003100959.1 437786..438934(+) (pilU) [Pseudomonas aeruginosa strain PAO1-N]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=701583 NHZ69_RS02030 WP_003100959.1 437786..438934(+) (pilU) [Pseudomonas aeruginosa strain PAO1-N]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382