Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   NF676_RS02695 Genome accession   NZ_CP099598
Coordinates   471852..472916 (+) Length   354 a.a.
NCBI ID   WP_056787331.1    Uniprot ID   A0A5E7N9B5
Organism   Pseudomonas siliginis strain OTU6BANIB1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 466852..477916
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NF676_RS02685 (NF676_02685) - 467599..468867 (+) 1269 WP_016772053.1 malic enzyme-like NAD(P)-binding protein -
  NF676_RS02690 (NF676_02690) - 469188..471635 (-) 2448 WP_056787328.1 penicillin-binding protein 1A -
  NF676_RS02695 (NF676_02695) pilM 471852..472916 (+) 1065 WP_056787331.1 pilus assembly protein PilM Machinery gene
  NF676_RS02700 (NF676_02700) - 472916..473482 (+) 567 WP_056787334.1 PilN domain-containing protein -
  NF676_RS02705 (NF676_02705) pilO 473479..474102 (+) 624 WP_056787337.1 type 4a pilus biogenesis protein PilO -
  NF676_RS02710 (NF676_02710) - 474099..474623 (+) 525 WP_056787340.1 pilus assembly protein PilP -
  NF676_RS02715 (NF676_02715) pilQ 474637..476724 (+) 2088 WP_056787342.1 type IV pilus secretin PilQ Machinery gene
  NF676_RS02720 (NF676_02720) aroK 476729..477247 (+) 519 WP_024011270.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 38017.52 Da        Isoelectric Point: 4.7453

>NTDB_id=700890 NF676_RS02695 WP_056787331.1 471852..472916(+) (pilM) [Pseudomonas siliginis strain OTU6BANIB1]
MLGLFNKKTNALLGIDISSTSVKLLELSRQGERYRVEAYAVEPLPANAVVEKNIADLEGVGQALSRLLVKARTGVKSVAV
AVAGSAVITKVIEMDAGLSDDELENQLKIEADQYIPYPLDEVAIDFEVQGVSPRNPDRVNVLLAACRKENVEVREAALAL
AGLTARVVDVEAYALERSFGLLATHLAASQERLTVAVVDIGATMTTLSVLHNGKIIYTREQLFGGRQLTEEIQRRYGLTV
EQAGLAKKQGGLPDDYVSEVLQPFREALVQQVSRSLQFFFASGQYNAVDHILLAGGTASVPGLDRLIEQRLDTPTQVANP
FADMALGSKVNAGALASDAPALMIACGLALRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=700890 NF676_RS02695 WP_056787331.1 471852..472916(+) (pilM) [Pseudomonas siliginis strain OTU6BANIB1]
GTGCTGGGACTCTTCAATAAAAAGACCAATGCGTTACTGGGGATCGACATCAGCTCCACGTCGGTGAAGCTGCTGGAACT
GAGCCGTCAGGGCGAGCGCTACCGGGTCGAGGCCTATGCGGTGGAGCCCTTGCCGGCGAACGCCGTGGTCGAAAAGAACA
TCGCCGACCTCGAGGGCGTCGGCCAGGCGCTGAGCCGGTTGCTGGTGAAAGCGCGAACCGGGGTCAAGAGCGTGGCGGTG
GCAGTGGCCGGTTCTGCGGTGATCACCAAGGTCATCGAGATGGACGCCGGGCTGTCCGACGACGAACTGGAAAACCAGCT
CAAGATCGAGGCCGACCAATACATTCCCTATCCGCTGGACGAAGTCGCCATCGATTTTGAAGTCCAGGGCGTCTCGCCGC
GCAACCCGGATCGGGTCAACGTGCTGCTTGCCGCCTGTCGCAAGGAAAACGTCGAGGTACGTGAAGCGGCGCTGGCGCTC
GCCGGCCTGACCGCGCGGGTGGTGGATGTCGAGGCCTACGCGCTGGAGCGCTCGTTCGGCCTGCTGGCAACGCATCTGGC
GGCCTCCCAGGAGCGCCTGACCGTGGCGGTGGTCGACATCGGCGCCACCATGACCACCCTCAGCGTCCTGCACAACGGCA
AGATCATCTATACCCGCGAGCAATTGTTTGGCGGGCGCCAACTCACCGAGGAGATCCAGCGCCGCTACGGCCTCACGGTC
GAACAGGCGGGCCTGGCGAAAAAACAGGGCGGGCTGCCTGACGATTACGTCAGCGAGGTCTTGCAGCCATTTCGCGAGGC
GCTGGTGCAGCAAGTCTCGCGTTCGCTGCAGTTCTTCTTCGCCTCCGGGCAGTACAACGCGGTCGACCATATCCTGTTGG
CCGGCGGCACCGCCTCGGTGCCCGGGCTGGACCGTCTGATCGAACAGCGTCTGGACACGCCGACGCAGGTCGCCAACCCA
TTCGCCGACATGGCCTTGGGCAGCAAGGTCAACGCAGGAGCGCTGGCCAGCGACGCGCCAGCCTTGATGATTGCCTGCGG
GCTCGCGCTCAGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5E7N9B5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

56.215

100

0.562
  comM Acinetobacter nosocomialis M2

56.215

100

0.562
  comM Acinetobacter baylyi ADP1

54.52

100

0.545
  pilM Legionella pneumophila strain ERS1305867

48.588

100

0.486