Detailed information NTG0700751

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: PCP73_RS10485
• Genome accession: NZ_CP115271
• Coordinates: 2245044..2246108 (-)
• Length: 354 a.a.
• NCBI ID: WP_003110888.1
• Uniprot ID: A0A0H2ZID6
• Organism: Pseudomonas aeruginosa strain F018
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2240044..2251108

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PCP73_RS10460 (PCP73_10455)	aroK	2240579..2241097 (-)	519	WP_003095833.1	shikimate kinase AroK	-
PCP73_RS10465 (PCP73_10460)	pilQ	2241109..2243253 (-)	2145	WP_003114575.1	type 4a pilus secretin PilQ	Machinery gene
PCP73_RS10470 (PCP73_10465)	comL/pilP	2243307..2243831 (-)	525	WP_003095836.1	type 4a pilus biogenesis lipoprotein PilP	Machinery gene
PCP73_RS10475 (PCP73_10470)	pilO	2243828..2244451 (-)	624	WP_003103268.1	type 4a pilus biogenesis protein PilO	Machinery gene
PCP73_RS10480 (PCP73_10475)	pilN	2244448..2245044 (-)	597	WP_003095839.1	type 4a pilus biogenesis protein PilN	Machinery gene
PCP73_RS10485 (PCP73_10480)	pilM	2245044..2246108 (-)	1065	WP_003110888.1	type IV pilus assembly protein PilM	Machinery gene
PCP73_RS10490 (PCP73_10485)	-	2246293..2248764 (+)	2472	WP_003095841.1	penicillin-binding protein 1A	-
PCP73_RS10495 (PCP73_10490)	-	2248869..2250137 (-)	1269	WP_003103278.1	malic enzyme	-

Sequence

Protein

• Length: 354 a.a.
• Molecular weight: 37983.48 Da
• Isoelectric Point: 4.5405

>NTDB_id=700751 PCP73_RS10485 WP_003110888.1 2245044..2246108(-) (pilM) [Pseudomonas aeruginosa strain F018]
MLGLIKKKANTLLGIDISSTSVKLLELSRSGGRYKVEAYAVEPLPPNAVVEKNIVELEGVGQALSRVLVKAKTNLKSAVV
AVAGSAVITKTIEMEAGLSEDELENQLKIEADQYIPYPLEEVAIDFEVQGLSARNPERVDVLLAACRKENVEVREAALAL
AGLTAKVVDVEAYALERSYALLSSQLGADTDQLTVAVVDIGATMTTLSVLHNGRTIYTREQLFGGRQLTEEIQRRYGLSV
EEAGLAKKQGGLPDDYDSEVLRPFKDAVVQQVSRSLQFFFAAGQFNDVDYIVLAGGTASIQDLDRLIQQKIGTPTLVANP
FADMALNGKVNAGALASDAPALMIACGLALRSFD

Nucleotide

• Length: 1065 bp

>NTDB_id=700751 PCP73_RS10485 WP_003110888.1 2245044..2246108(-) (pilM) [Pseudomonas aeruginosa strain F018]
GTGCTAGGGCTCATAAAGAAGAAAGCGAACACCTTGCTGGGGATCGACATCAGCTCGACCTCGGTAAAGCTCCTTGAGTT
GAGCCGCTCCGGAGGCCGCTACAAAGTGGAGGCCTATGCCGTCGAGCCGCTTCCGCCGAATGCAGTCGTGGAAAAGAACA
TCGTCGAGCTGGAAGGGGTCGGCCAGGCGTTGTCACGGGTGCTGGTCAAGGCGAAAACCAACCTGAAGTCGGCCGTGGTC
GCCGTCGCCGGTTCGGCGGTGATCACCAAGACCATCGAGATGGAGGCCGGGCTTTCCGAGGATGAACTGGAAAACCAGCT
GAAGATCGAGGCCGACCAGTACATCCCCTATCCGCTGGAGGAAGTCGCCATCGACTTCGAGGTCCAGGGGCTCTCCGCGC
GCAATCCCGAGCGCGTGGACGTACTCCTGGCCGCCTGTCGGAAAGAAAACGTCGAGGTTCGCGAAGCGGCCCTGGCGCTG
GCCGGCCTGACCGCCAAGGTGGTCGACGTCGAGGCCTACGCGCTGGAGCGCTCCTATGCGCTGCTGAGCAGCCAGCTGGG
TGCCGACACCGACCAGTTGACCGTGGCGGTAGTGGACATCGGCGCGACCATGACCACCCTGAGCGTCCTGCACAACGGAC
GCACCATCTATACCCGCGAACAGCTGTTCGGCGGTCGCCAGCTCACCGAGGAGATCCAGCGGCGTTACGGACTCTCGGTG
GAGGAAGCCGGTCTCGCCAAGAAGCAGGGCGGTCTTCCGGATGACTACGACAGCGAAGTTCTGCGTCCGTTCAAGGACGC
CGTGGTGCAGCAGGTTTCCCGCTCGCTGCAGTTCTTCTTCGCCGCCGGACAGTTCAACGACGTCGACTACATCGTCCTGG
CGGGCGGTACGGCGTCTATCCAGGATCTCGATCGGTTGATCCAGCAGAAGATCGGCACCCCGACCCTGGTCGCCAACCCG
TTCGCCGACATGGCGCTGAACGGCAAGGTGAATGCCGGCGCCCTGGCCAGCGACGCTCCGGCGTTGATGATCGCCTGCGG
CCTGGCGTTGAGGAGTTTCGACTGA

Domains

Predicted by InterProScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-353)

3D structure

• AlphaFold DB: A0A0H2ZID6

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	55.899	100	0.562
comM	Acinetobacter nosocomialis M2	55.899	100	0.562
comM/pilM	Acinetobacter baylyi ADP1	51.977	100	0.52
pilM	Legionella pneumophila strain ERS1305867	46.61	100	0.466