Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   ALSL_RS10350 Genome accession   NZ_AP018560
Coordinates   2200291..2201349 (-) Length   352 a.a.
NCBI ID   WP_126538896.1    Uniprot ID   -
Organism   Aerosticca soli strain Dysh456     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2195291..2206349
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ALSL_RS10325 (ALSL_2075) - 2195947..2196201 (-) 255 WP_126538888.1 hypothetical protein -
  ALSL_RS10330 (ALSL_2076) - 2196312..2198486 (-) 2175 WP_126540213.1 type IV pilus secretin PilQ -
  ALSL_RS10335 (ALSL_2077) - 2198509..2199042 (-) 534 WP_126538890.1 pilus assembly protein PilP -
  ALSL_RS10340 (ALSL_2078) pilO 2199039..2199695 (-) 657 WP_126538892.1 type 4a pilus biogenesis protein PilO -
  ALSL_RS10345 (ALSL_2079) - 2199692..2200291 (-) 600 WP_126538894.1 PilN domain-containing protein -
  ALSL_RS10350 (ALSL_2080) pilM 2200291..2201349 (-) 1059 WP_126538896.1 pilus assembly protein PilM Machinery gene
  ALSL_RS10355 (ALSL_2081) - 2201567..2203705 (-) 2139 WP_231700331.1 beta-agarase -
  ALSL_RS10360 (ALSL_2082) - 2203717..2205045 (-) 1329 WP_126538900.1 amino acid permease -

Sequence

Protein


Download         Length: 352 a.a.        Molecular weight: 37873.48 Da        Isoelectric Point: 4.5922

>NTDB_id=70066 ALSL_RS10350 WP_126538896.1 2200291..2201349(-) (pilM) [Aerosticca soli strain Dysh456]
MGLFTPKRPPLIGVDISSTAVKLLQLSQSGGRYRVEHYAVEPLPPNAVVEKNIVEVEAVGEAIRRALARSGSKAKHAAAA
VAGSAVITRIVPMPADLSEDDLEGQILVDANQYIPYPIEEVSLDFEVLGPVRDNPEMNNVLLAASRTENVDMRVAALELG
GLKAQVIDVEAFALENAFALLVDQLNVGRDALVALVDIGATMTTLSVLKSQRTIYSREQVFGGRQLTDEIMRRYGLSYEE
AGRAKRKGGLPESYESEVLEPFKEALVQQISRLLQFFFAGSEYGKVDEVVLAGGSASIDGIGRMLEEQLGVPCVVANPLA
RMALSSRVQAQALAQDAPALMIAVGLALRSFD

Nucleotide


Download         Length: 1059 bp        

>NTDB_id=70066 ALSL_RS10350 WP_126538896.1 2200291..2201349(-) (pilM) [Aerosticca soli strain Dysh456]
GTGGGGTTGTTCACGCCCAAAAGGCCGCCACTGATCGGTGTCGACATCAGCTCGACCGCGGTCAAGCTGCTCCAGCTCAG
CCAGTCCGGTGGTCGCTACCGCGTCGAGCATTACGCGGTCGAGCCGCTGCCGCCCAATGCCGTGGTGGAAAAAAACATCG
TCGAGGTCGAGGCGGTGGGCGAGGCCATCCGTCGCGCGCTCGCCCGTTCCGGCTCGAAAGCCAAGCATGCCGCAGCGGCG
GTGGCCGGATCGGCGGTCATCACGCGCATCGTGCCGATGCCGGCCGACCTGTCCGAAGACGATCTCGAAGGACAGATCCT
GGTCGATGCCAACCAGTACATTCCCTATCCGATCGAGGAAGTCAGTCTCGACTTCGAGGTCTTGGGTCCGGTCCGCGACA
ACCCGGAAATGAACAACGTGCTGCTGGCGGCCTCGCGCACCGAGAACGTCGACATGCGCGTGGCCGCGCTCGAGCTCGGT
GGCCTCAAGGCACAGGTGATCGATGTCGAGGCCTTCGCGCTCGAAAACGCCTTCGCGCTGCTCGTCGATCAGCTCAACGT
GGGGCGCGATGCCCTGGTCGCCCTGGTCGACATCGGCGCCACCATGACCACGCTCTCGGTGCTGAAGAGCCAGCGCACCA
TCTATTCACGCGAGCAGGTCTTCGGCGGCCGCCAGCTCACCGACGAGATCATGCGCCGCTACGGCCTCTCCTATGAAGAG
GCCGGCCGCGCCAAGCGCAAGGGTGGCCTGCCTGAGTCCTACGAGAGCGAAGTCCTCGAGCCGTTCAAGGAAGCGCTGGT
GCAGCAGATCAGCCGTCTCCTGCAGTTCTTCTTCGCCGGCAGCGAGTATGGCAAGGTCGATGAAGTGGTGCTCGCGGGCG
GCAGCGCCTCGATCGACGGCATCGGCCGGATGCTGGAGGAACAGCTCGGCGTGCCTTGCGTGGTGGCCAATCCGCTTGCG
CGCATGGCGCTGTCATCCCGCGTCCAGGCGCAAGCGCTCGCCCAGGATGCGCCGGCGTTGATGATCGCCGTCGGGCTCGC
CTTGAGGAGCTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(13-351)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

53.801

97.159

0.523
  comM Acinetobacter nosocomialis M2

53.509

97.159

0.52
  comM Acinetobacter baylyi ADP1

52.011

98.864

0.514
  pilM Legionella pneumophila strain ERS1305867

43.343

100

0.435

Multiple sequence alignment