Detailed information NTG0700358

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: DVS74_RS04550
• Genome accession: NZ_CP099516
• Coordinates: 1015457..1016587 (+)
• Length: 376 a.a.
• NCBI ID: WP_004084950.1
• Uniprot ID: -
• Organism: Xylella fastidiosa subsp. multiplex strain ESVL
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1010457..1021587

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
DVS74_RS04530 (DVS74_004505)	-	1010781..1011605 (+)	825	WP_004084943.1	glycosyltransferase family 2 protein	-
DVS74_RS04535 (DVS74_004510)	-	1012222..1013049 (-)	828	WP_004084945.1	glycosyltransferase family 2 protein	-
DVS74_RS04540 (DVS74_004515)	-	1013434..1013646 (-)	213	WP_021358439.1	hypothetical protein	-
DVS74_RS04545 (DVS74_004520)	pilT	1014268..1015302 (+)	1035	WP_004084948.1	type IV pilus twitching motility protein PilT	Machinery gene
DVS74_RS04550 (DVS74_004525)	pilU	1015457..1016587 (+)	1131	WP_004084950.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
DVS74_RS04555 (DVS74_004530)	-	1017256..1018224 (-)	969	WP_004084952.1	helix-turn-helix transcriptional regulator	-
DVS74_RS04560 (DVS74_004535)	-	1019007..1019816 (+)	810	WP_021358437.1	DUF481 domain-containing protein	-
DVS74_RS04565 (DVS74_004540)	hemC	1019870..1020787 (-)	918	WP_004084955.1	hydroxymethylbilane synthase	-
DVS74_RS04570 (DVS74_004545)	-	1020795..1021553 (-)	759	WP_004084957.1	LytR/AlgR family response regulator transcription factor	-

Sequence

Protein

• Length: 376 a.a.
• Molecular weight: 42402.63 Da
• Isoelectric Point: 6.3881

>NTDB_id=700358 DVS74_RS04550 WP_004084950.1 1015457..1016587(+) (pilU) [Xylella fastidiosa subsp. multiplex strain ESVL]
MTTIDFTSLLKLMAHQKASDLFITCGMPPSMKIHGNITPITQVPLTSEQSRDIVFNIMSEAQREEFEKVHECNFAIDITS
IGRFRVNAFYQRNQIGMVLRRIEMSIPTFEKLKLPQVAKELVMEKRGIIIIVGATGSGKSTSMASMINHRNHNSTGHIVT
IEDPIEFIHKHERCIITQREIGTDTDSWENALKNTLRQAPDVIVIGEVRTQETMDHAISFSETGHLVMCTLHANNANQAI
DRIINFFPEERRSQLLIDLSLNLKGIIAQQLIPSPDGKERCLAAEVLRDSPLVQDYIRVGEIHKLKEVMQNPNDLGMTTF
DQSLVELYHSGAINYKEALRHADSQNEVRLRIKLSDGKDAKTLSQGFDGVEIAEMS

Nucleotide

• Length: 1131 bp

>NTDB_id=700358 DVS74_RS04550 WP_004084950.1 1015457..1016587(+) (pilU) [Xylella fastidiosa subsp. multiplex strain ESVL]
ATGACTACCATCGATTTCACATCCTTACTTAAGCTGATGGCGCACCAAAAAGCGTCGGATTTATTTATTACCTGTGGAAT
GCCGCCTTCAATGAAGATTCACGGCAATATCACTCCAATCACACAAGTACCGCTGACTTCAGAGCAAAGCCGTGACATAG
TCTTCAATATCATGAGTGAAGCGCAGCGCGAGGAATTCGAGAAGGTCCACGAATGCAACTTCGCAATTGACATCACCAGC
ATTGGTCGCTTCCGTGTCAACGCTTTCTATCAGCGTAACCAAATTGGTATGGTGTTACGCAGAATCGAGATGTCTATTCC
CACCTTCGAAAAACTGAAGCTCCCGCAGGTCGCTAAGGAGCTCGTCATGGAGAAGCGCGGCATCATCATCATCGTGGGTG
CCACGGGTAGTGGTAAATCGACCTCAATGGCATCAATGATCAACCACCGTAATCACAACTCAACAGGACATATCGTCACT
ATCGAAGATCCCATTGAGTTTATTCACAAGCACGAGAGATGCATCATCACCCAACGCGAAATAGGTACCGACACCGACAG
TTGGGAAAATGCCTTGAAAAATACCCTACGCCAAGCCCCAGATGTGATTGTGATTGGTGAGGTGCGTACTCAAGAGACAA
TGGATCATGCCATCTCATTCTCCGAAACTGGCCATTTGGTCATGTGTACCCTACATGCCAACAACGCCAATCAAGCAATA
GACCGCATTATTAACTTCTTCCCGGAAGAGCGGCGCAGCCAATTACTGATAGATCTGTCGCTAAATCTAAAAGGCATCAT
CGCTCAACAATTGATCCCATCTCCGGATGGTAAAGAGCGCTGTTTAGCAGCGGAAGTTTTGCGCGACAGTCCATTAGTAC
AGGACTACATACGCGTAGGAGAGATCCATAAACTCAAAGAAGTCATGCAAAACCCCAACGATCTTGGAATGACGACATTC
GATCAAAGCTTGGTCGAACTCTATCATAGCGGAGCCATTAACTATAAAGAGGCACTACGCCATGCTGATTCGCAGAACGA
GGTACGCCTGCGTATTAAACTCTCCGATGGAAAAGACGCTAAAACTCTGAGTCAAGGATTCGATGGAGTGGAAATTGCAG
AAATGTCATAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (114-273)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	61.853	97.606	0.604
pilU	Acinetobacter baylyi ADP1	60.169	94.149	0.566
pilU	Vibrio cholerae strain A1552	51.404	94.681	0.487
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	41.484	96.809	0.402