Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   NG745_RS12465 Genome accession   NZ_CP099465
Coordinates   2549463..2550077 (-) Length   204 a.a.
NCBI ID   WP_015240245.1    Uniprot ID   -
Organism   Bacillus velezensis strain Ag75     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2544463..2555077
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NG745_RS12435 (NG745_12435) rpsT 2544766..2545032 (+) 267 WP_003152876.1 30S ribosomal protein S20 -
  NG745_RS12440 (NG745_12440) holA 2545049..2546090 (-) 1042 Protein_2418 DNA polymerase III subunit delta -
  NG745_RS12445 (NG745_12445) - 2546130..2546281 (-) 152 Protein_2419 hypothetical protein -
  NG745_RS12450 (NG745_12450) - 2546322..2546456 (+) 135 WP_003152870.1 YqzM family protein -
  NG745_RS12455 (NG745_12455) comEC 2546475..2548826 (-) 2352 WP_098081592.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  NG745_RS12460 (NG745_12460) - 2548827..2549396 (-) 570 WP_003152868.1 ComE operon protein 2 -
  NG745_RS12465 (NG745_12465) comEA 2549463..2550077 (-) 615 WP_015240245.1 helix-hairpin-helix domain-containing protein Machinery gene
  NG745_RS12470 (NG745_12470) comER 2550136..2550957 (+) 822 WP_015240246.1 late competence protein ComER -
  NG745_RS12475 (NG745_12475) - 2551026..2551763 (-) 738 WP_015240247.1 class I SAM-dependent methyltransferase -
  NG745_RS12480 (NG745_12480) rsfS 2551760..2552116 (-) 357 WP_007408260.1 ribosome silencing factor -
  NG745_RS12485 (NG745_12485) yqeK 2552140..2552694 (-) 555 WP_061860724.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  NG745_RS12490 (NG745_12490) - 2552684..2553253 (-) 570 WP_098081593.1 nicotinate-nucleotide adenylyltransferase -
  NG745_RS12495 (NG745_12495) yhbY 2553264..2553554 (-) 291 WP_098081594.1 ribosome assembly RNA-binding protein YhbY -
  NG745_RS12500 (NG745_12500) aroE 2553548..2554390 (-) 843 WP_003152856.1 shikimate dehydrogenase -

Sequence

Protein


Download         Length: 204 a.a.        Molecular weight: 22209.32 Da        Isoelectric Point: 5.5211

>NTDB_id=699886 NG745_RS12465 WP_015240245.1 2549463..2550077(-) (comEA) [Bacillus velezensis strain Ag75]
MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTAAGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide


Download         Length: 615 bp        

>NTDB_id=699886 NG745_RS12465 WP_015240245.1 2549463..2550077(-) (comEA) [Bacillus velezensis strain Ag75]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCGGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCAGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTAAAAGGGGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGGGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGCACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CGGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAGAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(141-202)

SLBB domain (SLBB)

(68-122)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

58.854

94.118

0.554
  comEA/celA/cilE Streptococcus pneumoniae TIGR4

35.545

100

0.368
  comEA/celA/cilE Streptococcus mitis SK321

35.071

100

0.363