Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEC   Type   Machinery gene
Locus tag   NG745_RS12455 Genome accession   NZ_CP099465
Coordinates   2546475..2548826 (-) Length   783 a.a.
NCBI ID   WP_098081592.1    Uniprot ID   -
Organism   Bacillus velezensis strain Ag75     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2541475..2553826
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NG745_RS12420 (NG745_12420) - 2541844..2542179 (-) 336 WP_060562638.1 YqxA family protein -
  NG745_RS12425 (NG745_12425) - 2542196..2543389 (-) 1194 WP_007612673.1 stage II sporulation protein P -
  NG745_RS12430 (NG745_12430) gpr 2543457..2544563 (-) 1107 WP_007408268.1 GPR endopeptidase -
  NG745_RS12435 (NG745_12435) rpsT 2544766..2545032 (+) 267 WP_003152876.1 30S ribosomal protein S20 -
  NG745_RS12440 (NG745_12440) holA 2545049..2546090 (-) 1042 Protein_2418 DNA polymerase III subunit delta -
  NG745_RS12445 (NG745_12445) - 2546130..2546281 (-) 152 Protein_2419 hypothetical protein -
  NG745_RS12450 (NG745_12450) - 2546322..2546456 (+) 135 WP_003152870.1 YqzM family protein -
  NG745_RS12455 (NG745_12455) comEC 2546475..2548826 (-) 2352 WP_098081592.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  NG745_RS12460 (NG745_12460) - 2548827..2549396 (-) 570 WP_003152868.1 ComE operon protein 2 -
  NG745_RS12465 (NG745_12465) comEA 2549463..2550077 (-) 615 WP_015240245.1 helix-hairpin-helix domain-containing protein Machinery gene
  NG745_RS12470 (NG745_12470) comER 2550136..2550957 (+) 822 WP_015240246.1 late competence protein ComER -
  NG745_RS12475 (NG745_12475) - 2551026..2551763 (-) 738 WP_015240247.1 class I SAM-dependent methyltransferase -
  NG745_RS12480 (NG745_12480) rsfS 2551760..2552116 (-) 357 WP_007408260.1 ribosome silencing factor -
  NG745_RS12485 (NG745_12485) yqeK 2552140..2552694 (-) 555 WP_061860724.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  NG745_RS12490 (NG745_12490) - 2552684..2553253 (-) 570 WP_098081593.1 nicotinate-nucleotide adenylyltransferase -
  NG745_RS12495 (NG745_12495) yhbY 2553264..2553554 (-) 291 WP_098081594.1 ribosome assembly RNA-binding protein YhbY -

Sequence

Protein


Download         Length: 783 a.a.        Molecular weight: 86683.55 Da        Isoelectric Point: 9.0097

>NTDB_id=699884 NG745_RS12455 WP_098081592.1 2546475..2548826(-) (comEC) [Bacillus velezensis strain Ag75]
MKYKYLLLPLAAVSATAGIAAAHVFWGMLLFLLYLLFIIVKTKRHALVIVCLVSFCLYFFLYTVCDAANVTRYQAGSYTE
QAVITNIPKVDGAKMSAVIRTHDKEKWAASYKIRSLEEKRLIEQLEPGMRCTFTGSLEQPAHATVPGGFDYKEYLYSQQI
HWLFSVTSIEQCEKSKQPLFKLLNIRKNLISIIRNHVPESSAGIVEALTLGERFSIEDDILSAYQNLGVVHLMAISGMHV
GLITAGLFYALIRIGLTREKAGILLLLFLPVYTLLSGAAPSVLRASLMLGFYIAGTLVKRGIHSSAALSLSYLLLLLFNP
YFLWQAGFQLSFAVSASLILSSSILKKAGKSRLAGLAMASLIAELSSLPFLLYHFQQISLVSFPMNMVMVPFYTLFVIPV
SVISFLLLLLSRQMGECLFDMFDLVMKPVHDFITYAASVDLFTMIVSKPDFVSLLLLAVSVFTLFAALEKGGFLKLRKSA
LFFCAVLAYLICRPYFSPWGEADMLDIGQGDSLFISAPHRKGTVMVDTGGVIAYPGESWKEKRHPYSIGEKVLIPFLNGK
GVKKLDALILTHADQDHIGEAGVLIKNHRVKRLIVPVGFVKEPKDQNILNMAKENNIPVADAKRGDTITAGDLQFQVLSP
ESSDGRSKNDSSLVLWTVLGGVSWLLTGDLESDGETEVLKTYPNLKADILKAGHHGSKSSTSEAFLKQLQPEAALISAGK
ENRYHHPHEEVLDRLKAYSVNVLRTDISGTIQYRFKKGAGTFSVFPPYDIEETRAQEVKKTAD

Nucleotide


Download         Length: 2352 bp        

>NTDB_id=699884 NG745_RS12455 WP_098081592.1 2546475..2548826(-) (comEC) [Bacillus velezensis strain Ag75]
ATGAAATATAAATACCTTCTTCTGCCTCTGGCGGCGGTTTCTGCAACTGCGGGAATTGCCGCCGCTCATGTCTTCTGGGG
TATGCTCCTTTTTCTTCTGTATCTTCTCTTTATTATTGTAAAAACAAAGCGCCATGCTCTGGTTATCGTCTGCCTCGTTT
CTTTTTGTCTTTATTTCTTTCTTTATACGGTTTGTGACGCTGCGAATGTAACGCGCTATCAGGCCGGCAGTTATACTGAA
CAGGCCGTCATCACTAATATTCCGAAGGTTGACGGAGCGAAAATGTCAGCCGTTATCCGTACACATGACAAGGAAAAATG
GGCGGCTTCGTACAAAATCCGGTCTCTTGAGGAAAAGAGACTCATTGAACAGCTTGAACCGGGGATGCGCTGCACGTTTA
CAGGCTCTCTGGAACAGCCTGCACATGCGACGGTTCCCGGAGGTTTTGATTATAAGGAATATCTTTACTCTCAGCAGATT
CACTGGTTATTTTCCGTGACTTCCATTGAGCAGTGTGAAAAATCCAAACAGCCGCTGTTTAAACTGCTGAACATCAGAAA
AAATTTGATTTCGATCATTCGGAATCACGTGCCTGAATCTTCCGCCGGAATTGTTGAAGCGCTGACCTTAGGTGAAAGAT
TTTCTATAGAGGACGATATACTGAGTGCGTATCAAAATTTGGGAGTCGTTCATTTAATGGCGATTTCCGGAATGCATGTC
GGTCTTATTACGGCGGGATTATTTTATGCTCTGATCAGAATCGGGCTGACAAGAGAAAAGGCGGGAATTTTGCTGCTGCT
GTTTTTGCCGGTGTATACGCTGCTGAGCGGTGCCGCCCCATCCGTATTGCGCGCATCCCTCATGCTGGGATTTTATATCG
CCGGAACTCTTGTTAAACGCGGCATTCATTCCTCTGCTGCATTGTCCCTGTCTTATCTGCTGCTTCTGCTGTTTAATCCT
TACTTCCTTTGGCAGGCGGGCTTCCAGCTTTCCTTTGCGGTAAGCGCCTCTTTAATTCTGTCATCCTCCATTTTAAAGAA
AGCAGGGAAAAGCAGACTTGCCGGGCTTGCAATGGCTTCATTGATTGCGGAGCTCAGCTCACTTCCGTTTCTTCTCTATC
ATTTTCAGCAGATTTCACTTGTCAGTTTTCCGATGAATATGGTGATGGTGCCATTTTATACGTTATTTGTCATTCCGGTT
TCTGTCATCAGTTTCCTTCTTCTTTTACTCTCAAGGCAGATGGGAGAATGTTTGTTTGATATGTTTGACCTTGTGATGAA
GCCTGTGCATGATTTCATTACATATGCGGCATCCGTTGATTTATTTACTATGATTGTGTCAAAGCCTGACTTTGTTTCCC
TTCTTCTGCTTGCGGTTTCCGTTTTTACGCTTTTTGCGGCTTTGGAAAAGGGAGGTTTTTTAAAACTCAGGAAATCGGCT
CTTTTTTTCTGCGCGGTTTTGGCTTATTTAATATGCCGTCCGTATTTCAGTCCATGGGGAGAAGCGGATATGCTTGATAT
CGGGCAGGGAGACTCACTGTTCATAAGTGCGCCGCACCGCAAAGGGACCGTAATGGTTGATACAGGGGGAGTGATTGCTT
ATCCCGGAGAATCATGGAAAGAAAAACGTCACCCGTATTCTATCGGCGAGAAGGTTTTGATTCCATTTTTAAACGGAAAA
GGGGTGAAAAAGCTGGATGCACTGATTTTAACCCATGCGGATCAAGATCACATCGGGGAAGCCGGAGTGTTAATCAAAAA
TCATAGAGTCAAACGGTTAATTGTCCCCGTGGGATTCGTAAAAGAACCGAAGGATCAGAACATATTAAATATGGCGAAAG
AAAACAACATTCCCGTTGCCGATGCAAAGCGGGGCGACACCATTACAGCCGGTGATCTTCAGTTTCAGGTGCTGTCTCCG
GAGTCGTCTGACGGAAGGAGTAAAAATGATTCGTCACTGGTGCTTTGGACGGTTTTAGGCGGAGTGAGCTGGCTTTTGAC
GGGAGATTTAGAATCGGACGGCGAAACAGAAGTGCTGAAAACGTATCCGAATCTGAAGGCTGATATATTGAAGGCGGGTC
ATCACGGCAGCAAAAGCTCAACGAGTGAAGCCTTTTTGAAGCAGCTTCAGCCGGAAGCAGCGCTGATTTCAGCAGGAAAA
GAGAATCGATACCATCATCCGCATGAAGAAGTGCTGGATCGTTTGAAGGCGTACTCTGTCAATGTGCTTCGCACCGATAT
CAGCGGAACGATTCAATACAGATTTAAAAAAGGCGCCGGAACGTTTTCCGTCTTCCCTCCATATGATATAGAAGAAACCA
GGGCGCAAGAAGTAAAAAAGACTGCCGATTGA

Domains

Predicted by InterproScan.

Competence protein (Competence)

(208-469)

Metallo-beta-lactamase superfamily (Lactamase_B)

(508-719)

Domain of unknown function (DUF4131) (DUF4131)

(23-171)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEC Bacillus subtilis subsp. subtilis str. 168

57.124

98.595

0.563