Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB   Type   Machinery gene
Locus tag   NF199_RS11760 Genome accession   NZ_CP099460
Coordinates   2440647..2441684 (-) Length   345 a.a.
NCBI ID   WP_032874014.1    Uniprot ID   -
Organism   Bacillus velezensis strain E     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2435647..2446684
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NF199_RS11710 tasA 2435880..2436665 (-) 786 WP_032874027.1 biofilm matrix protein TasA -
  NF199_RS11715 sipW 2436730..2437314 (-) 585 WP_032874025.1 signal peptidase I SipW -
  NF199_RS11720 tapA 2437286..2437957 (-) 672 WP_265605828.1 amyloid fiber anchoring/assembly protein TapA -
  NF199_RS11725 - 2438216..2438545 (+) 330 WP_032874021.1 DUF3889 domain-containing protein -
  NF199_RS11730 - 2438586..2438765 (-) 180 WP_022552966.1 YqzE family protein -
  NF199_RS11735 comGG 2438822..2439199 (-) 378 WP_032874019.1 competence type IV pilus minor pilin ComGG Machinery gene
  NF199_RS11740 comGF 2439200..2439664 (-) 465 WP_223813077.1 competence type IV pilus minor pilin ComGF -
  NF199_RS11745 comGE 2439609..2439923 (-) 315 WP_032874016.1 competence type IV pilus minor pilin ComGE Machinery gene
  NF199_RS11750 comGD 2439907..2440344 (-) 438 WP_007612572.1 competence type IV pilus minor pilin ComGD Machinery gene
  NF199_RS11755 comGC 2440334..2440600 (-) 267 WP_042635730.1 competence type IV pilus major pilin ComGC Machinery gene
  NF199_RS11760 comGB 2440647..2441684 (-) 1038 WP_032874014.1 competence type IV pilus assembly protein ComGB Machinery gene
  NF199_RS11765 comGA 2441671..2442741 (-) 1071 WP_003153083.1 competence type IV pilus ATPase ComGA Machinery gene
  NF199_RS11770 - 2442938..2443888 (-) 951 WP_032874012.1 magnesium transporter CorA family protein -
  NF199_RS11775 - 2444034..2445335 (+) 1302 WP_032874010.1 hemolysin family protein -
  NF199_RS11780 - 2445373..2446203 (-) 831 WP_032874008.1 STAS domain-containing protein -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 39525.83 Da        Isoelectric Point: 9.5935

>NTDB_id=699790 NF199_RS11760 WP_032874014.1 2440647..2441684(-) (comGB) [Bacillus velezensis strain E]
MKRIKRTWPLKDQAAFLKRLGEMTEKGYSLIEGLRLLKLQLHKRQLAELTDGIRRLREGDAFYQVLEALSFHKEAVSICY
FAEKHGELPGAMKQSGDLLQRKLIQTNQIKKMLRYPMFLISSVCVMFYILQSLIIPQFSGIYQSMNMNTSGATAFIFAFF
RHFHEACALALSAAFCLFLYVWFLCKKKSPQDKMLIVVKIPLLGKAAVLFNSYFFSLQLSSLLKSGLSIYDSLTAFKEQS
FLPFYREEAEMLITRLKAGETIESALSGHPCYEKDLAAAVSHGQANGLLHRELYTYSQFMMERLEQNAAKYTGILQPVIY
GVVAGMILIVYMSMLMPMYQMMNQM

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=699790 NF199_RS11760 WP_032874014.1 2440647..2441684(-) (comGB) [Bacillus velezensis strain E]
ATGAAGCGGATTAAACGAACCTGGCCGTTGAAGGATCAAGCGGCTTTTTTGAAAAGGCTCGGTGAAATGACGGAAAAAGG
ATACAGTCTGATTGAAGGGCTAAGACTGCTGAAACTCCAGCTGCATAAACGTCAGCTTGCAGAGCTGACGGACGGAATCC
GCCGGCTGAGGGAAGGTGATGCGTTTTACCAAGTGCTTGAGGCACTGTCTTTTCACAAAGAAGCCGTCTCTATCTGCTAT
TTCGCTGAGAAACACGGGGAATTGCCGGGAGCGATGAAACAAAGCGGCGACTTGTTACAGAGAAAGCTCATACAGACGAA
TCAAATCAAAAAAATGCTCCGGTATCCGATGTTTTTAATATCTTCCGTCTGTGTCATGTTTTATATCCTGCAAAGTTTAA
TTATTCCGCAGTTTTCTGGGATTTATCAATCAATGAATATGAATACATCCGGTGCGACTGCTTTCATCTTTGCGTTTTTT
CGGCATTTTCATGAAGCCTGCGCACTGGCGCTGTCCGCCGCTTTCTGCTTGTTTTTGTACGTTTGGTTCTTATGTAAGAA
AAAATCCCCGCAAGATAAAATGCTGATTGTTGTGAAAATACCACTATTAGGAAAAGCGGCCGTTCTTTTTAACAGCTACT
TTTTCTCTTTACAGCTCAGCAGTCTTCTGAAATCCGGCCTTTCTATCTATGACAGCTTAACAGCTTTTAAAGAACAATCT
TTTCTGCCTTTTTATCGTGAAGAAGCAGAGATGCTCATTACACGTCTGAAAGCCGGAGAAACCATAGAATCCGCTCTTTC
CGGGCATCCGTGTTATGAAAAAGATCTTGCGGCTGCAGTCAGCCACGGACAGGCAAACGGCCTGCTTCACCGCGAACTTT
ATACGTACAGCCAGTTTATGATGGAGCGTCTTGAACAAAACGCGGCGAAGTATACCGGCATTCTCCAGCCTGTCATTTAC
GGTGTTGTGGCCGGTATGATTTTAATTGTCTACATGTCAATGCTGATGCCGATGTACCAGATGATGAACCAAATGTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(215-337)

Type II secretion system (T2SS), protein F (T2SSF)

(16-136)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB Bacillus subtilis subsp. subtilis str. 168

65.635

93.623

0.614