Detailed information NTG0697450

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: NDR94_RS20105
• Genome accession: NZ_CP098731
• Coordinates: 4302770..4303915 (-)
• Length: 381 a.a.
• NCBI ID: WP_019405534.1
• Uniprot ID: -
• Organism: Stutzerimonas stutzeri strain NRCB010
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4297770..4308915

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
NDR94_RS20080 (NDR94_20050)	-	4297847..4298863 (+)	1017	WP_011914964.1	aspartate carbamoyltransferase catalytic subunit	-
NDR94_RS20085 (NDR94_20055)	-	4298863..4300137 (+)	1275	WP_011914965.1	dihydroorotase	-
NDR94_RS20090 (NDR94_20060)	-	4300195..4300590 (-)	396	WP_011914966.1	NINE protein	-
NDR94_RS20095 (NDR94_20065)	-	4300726..4301940 (+)	1215	WP_041755962.1	putative DNA modification/repair radical SAM protein	-
NDR94_RS20100 (NDR94_20070)	-	4301940..4302773 (+)	834	WP_011914968.1	TIGR03915 family putative DNA repair protein	-
NDR94_RS20105 (NDR94_20075)	pilU	4302770..4303915 (-)	1146	WP_019405534.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
NDR94_RS20110 (NDR94_20080)	pilT	4303975..4305009 (-)	1035	WP_011914970.1	type IV pilus twitching motility protein PilT	Machinery gene
NDR94_RS20115 (NDR94_20085)	-	4305076..4305765 (+)	690	WP_011914971.1	YggS family pyridoxal phosphate-dependent enzyme	-
NDR94_RS20120 (NDR94_20090)	proC	4305776..4306600 (+)	825	WP_041755828.1	pyrroline-5-carboxylate reductase	-
NDR94_RS20125 (NDR94_20095)	-	4306610..4307200 (+)	591	WP_011914973.1	YggT family protein	-

Sequence

Protein

• Length: 381 a.a.
• Molecular weight: 42566.88 Da
• Isoelectric Point: 6.8858

>NTDB_id=697450 NDR94_RS20105 WP_019405534.1 4302770..4303915(-) (pilU) [Stutzerimonas stutzeri strain NRCB010]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGKIMPVSKTPMSPEMTRETVHGVMNEQQRREFTENHECNFAISARGIGR
FRVSAFYQRNLAGMVLRRIETNIPTIEELKLPDVLKKLSMTKRGLVLFVGATGTGKSTSLASMIGYRNKNSSGHIISIED
PIEFIHQHQNCIVTQREVGIDTSSFEVALKNTLRQAPDVILIGEIRTRETMDYAVAFAETGHLCLATLHANNANQALDRI
INFFPPDRHNQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLINTPLAADLIRKGEVHELKSLMKRSTELGMQTFDQA
LYNLYVQGEITYEDALLHADSANDLRLLIKLGSETDGEHLTSVSQGLSLEVSDEDPGRRFR

Nucleotide

• Length: 1146 bp

>NTDB_id=697450 NDR94_RS20105 WP_019405534.1 4302770..4303915(-) (pilU) [Stutzerimonas stutzeri strain NRCB010]
ATGGAATTCGAGAAACTTCTGCGCCTGATGGTCGAAAAAGGCGGCTCCGATCTGTTCATCACGGCTGGCGTGCCGCCATC
GATGAAGGTCAACGGCAAGATCATGCCGGTGAGCAAGACGCCCATGTCGCCGGAGATGACCCGGGAAACCGTGCACGGCG
TGATGAACGAGCAGCAGCGCCGCGAGTTCACCGAGAACCACGAATGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGTGTCAGCGCGTTCTATCAGCGAAACCTGGCCGGCATGGTGCTGCGTCGCATCGAGACCAACATCCCGACCATCGA
AGAACTCAAGCTGCCGGACGTCCTGAAGAAGCTGTCGATGACCAAACGTGGTCTGGTGCTGTTCGTCGGCGCCACCGGTA
CCGGCAAGTCGACGTCGCTGGCCTCGATGATCGGCTACCGCAACAAGAACTCCAGCGGGCACATCATCTCCATCGAAGAC
CCGATCGAATTCATCCACCAGCACCAGAATTGCATCGTCACCCAGCGCGAAGTGGGCATCGACACCAGCTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCCTCATCGGCGAGATCCGTACCCGCGAAACCATGGATTACG
CGGTCGCCTTCGCCGAAACCGGCCACCTGTGCCTGGCCACGCTGCACGCCAACAATGCCAACCAGGCGCTGGACCGCATC
ATCAACTTCTTCCCGCCGGACCGGCACAACCAGGTCTGGATGGACCTGTCGCTCAACCTCAAGGCCATCGTCGCCCAGCA
GCTGGTTCCGACCCCGGACGGCAAGGGTCGTCGTGCGGTCATCGAGGTGCTGATCAATACGCCGCTGGCGGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAATCGCTGATGAAGCGCTCCACCGAGCTGGGTATGCAGACCTTCGACCAGGCG
CTGTACAACCTCTACGTGCAGGGCGAGATCACCTACGAAGATGCCCTGCTGCATGCTGACTCGGCCAACGACCTGCGACT
GCTGATCAAGCTGGGTTCGGAAACCGACGGTGAGCACCTGACCAGCGTATCCCAGGGCCTGAGCCTGGAGGTCAGCGACG
AGGACCCGGGCCGGCGCTTCCGTTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (113-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	95.013	100	0.95
pilU	Acinetobacter baylyi ADP1	66.106	93.701	0.619
pilU	Vibrio cholerae strain A1552	59.429	91.864	0.546
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.953	88.976	0.391