Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   NDN13_RS14430 Genome accession   NZ_CP098480
Coordinates   3028510..3029691 (-) Length   393 a.a.
NCBI ID   WP_251115948.1    Uniprot ID   -
Organism   Acinetobacter sp. C32I     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3023510..3034691
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NDN13_RS14405 (NDN13_14400) rpmH 3024332..3024466 (+) 135 WP_000831329.1 50S ribosomal protein L34 -
  NDN13_RS14410 (NDN13_14405) rnpA 3024494..3024889 (+) 396 WP_251115945.1 ribonuclease P protein component -
  NDN13_RS14415 (NDN13_14410) yidD 3024899..3025222 (+) 324 WP_251115946.1 membrane protein insertion efficiency factor YidD -
  NDN13_RS14420 (NDN13_14415) yidC 3025225..3026985 (+) 1761 WP_159414015.1 membrane protein insertase YidC -
  NDN13_RS14425 (NDN13_14420) mnmE 3027100..3028455 (+) 1356 WP_251115947.1 tRNA uridine-5-carboxymethylaminomethyl(34) synthesis GTPase MnmE -
  NDN13_RS14430 (NDN13_14425) pilU 3028510..3029691 (-) 1182 WP_251115948.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  NDN13_RS14435 (NDN13_14430) - 3030126..3030824 (+) 699 WP_251115949.1 DsbC family protein -
  NDN13_RS14440 (NDN13_14435) - 3030879..3031130 (-) 252 WP_004656846.1 GlsB/YeaQ/YmgE family stress response membrane protein -
  NDN13_RS14445 (NDN13_14440) hpt 3031361..3031891 (-) 531 WP_016162105.1 hypoxanthine phosphoribosyltransferase -
  NDN13_RS14450 (NDN13_14445) guaD 3032104..3033432 (-) 1329 WP_251115950.1 guanine deaminase -
  NDN13_RS14455 (NDN13_14450) - 3033572..3034198 (+) 627 WP_251115951.1 nucleotidyltransferase family protein -

Sequence

Protein


Download         Length: 393 a.a.        Molecular weight: 44418.94 Da        Isoelectric Point: 6.5686

>NTDB_id=696162 NDN13_RS14430 WP_251115948.1 3028510..3029691(-) (pilU) [Acinetobacter sp. C32I]
MYNAELLEEAKTFMFHMLSKVVEYGGSDLFITADFPPSIKLQGLMRPLGQQALTADKTKLFAYSLMNEKQRQEFETEWEC
NFAINVPNVSRFRVNVFKQQLQIGMVIRTITSEIPTFQKLKLPESLKNVIMEKRGLVLVVGGTGSGKSTSLAAMIDHRNE
NSAGHIITVEDPVEYVHKHKKSMITHREVGVDCHSWHHALKNTLRQAPDVILIGEIRDTETMEHAIAFAETGHLCLGTLH
ANNANQALDRIINFFPEERRNQLLMDLSSNMKGIISQRLVRTQDGKGRRAAIEILLNTPLIADNILKGQFHALKEIMSKS
RELGMQTFDQALFELYNEGAISYDEALRNADSVNELRLQIKLKSTRQEGAPAAMMTALNVVQEQKTEEEQEEG

Nucleotide


Download         Length: 1182 bp        

>NTDB_id=696162 NDN13_RS14430 WP_251115948.1 3028510..3029691(-) (pilU) [Acinetobacter sp. C32I]
ATGTATAACGCGGAACTATTAGAAGAAGCCAAAACCTTTATGTTTCATATGCTTAGTAAAGTAGTGGAATATGGGGGATC
CGATTTATTTATCACCGCTGATTTTCCACCGAGCATTAAATTGCAAGGCCTGATGCGTCCATTGGGGCAACAGGCACTCA
CCGCAGATAAAACCAAGCTATTTGCCTATAGTTTGATGAATGAAAAACAGCGCCAAGAGTTTGAAACAGAATGGGAATGT
AATTTCGCAATCAATGTACCCAATGTTTCCCGTTTCCGTGTCAACGTGTTTAAACAACAGCTGCAAATCGGCATGGTGAT
TCGTACCATTACCTCAGAAATTCCAACCTTTCAAAAGCTGAAACTGCCAGAATCGCTGAAAAATGTGATTATGGAAAAGC
GTGGCTTGGTACTGGTTGTTGGGGGGACAGGCTCAGGCAAATCCACCTCTTTGGCGGCCATGATTGACCATCGTAATGAA
AACTCAGCGGGGCATATCATTACCGTTGAAGACCCAGTGGAATACGTGCATAAACACAAAAAATCGATGATTACCCATCG
TGAAGTGGGGGTTGATTGTCATTCATGGCACCACGCCTTGAAAAACACACTACGTCAGGCACCTGATGTGATTCTGATTG
GTGAAATCCGTGATACTGAAACCATGGAACACGCGATTGCCTTTGCGGAAACAGGACATCTTTGCTTAGGTACTTTACAC
GCCAACAATGCCAACCAAGCACTGGATCGAATTATTAACTTTTTCCCAGAAGAGCGTCGTAATCAGTTACTGATGGATCT
TTCATCCAATATGAAAGGGATTATTTCACAACGTCTGGTACGTACCCAAGATGGCAAAGGTCGTCGTGCTGCAATTGAAA
TTTTATTGAATACCCCATTGATTGCCGACAATATTCTCAAAGGACAGTTCCACGCACTGAAAGAGATTATGAGCAAATCG
CGTGAGCTGGGTATGCAAACCTTCGACCAAGCCTTATTTGAGCTGTATAACGAAGGCGCGATTAGCTATGATGAAGCCTT
ACGCAATGCAGATTCAGTCAATGAATTGCGTTTGCAAATCAAGCTGAAGAGTACTCGCCAAGAGGGTGCACCTGCCGCAA
TGATGACCGCATTAAATGTGGTACAAGAACAAAAAACAGAGGAAGAACAAGAAGAAGGTTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(103-282)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

60.632

88.55

0.537
  pilU Acinetobacter baylyi ADP1

55.908

88.295

0.494
  pilU Vibrio cholerae strain A1552

52.975

89.822

0.476
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.111

91.603

0.377