Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   NC849_RS08915 Genome accession   NZ_CP098462
Coordinates   1701971..1703647 (-) Length   558 a.a.
NCBI ID   WP_003703172.1    Uniprot ID   -
Organism   Neisseria gonorrhoeae strain 9460     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1696971..1708647
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NC849_RS08895 (NC849_08830) pilG 1698888..1700120 (+) 1233 WP_003689811.1 type 4 pilus assembly protein PilG Machinery gene
  NC849_RS08900 (NC849_08835) pilD 1700194..1701054 (+) 861 WP_260237063.1 A24 family peptidase Machinery gene
  NC849_RS08905 (NC849_08840) coaE 1701051..1701683 (+) 633 WP_003689816.1 dephospho-CoA kinase -
  NC849_RS08910 (NC849_08845) - 1701676..1701885 (+) 210 WP_003696096.1 DNA gyrase inhibitor YacG -
  NC849_RS08915 (NC849_08850) pilF 1701971..1703647 (-) 1677 WP_003703172.1 type IV-A pilus assembly ATPase PilB Machinery gene
  NC849_RS08925 (NC849_08860) - 1703985..1704431 (-) 447 WP_003689818.1 DUF441 domain-containing protein -
  NC849_RS08930 (NC849_08865) - 1704435..1705409 (-) 975 WP_003705489.1 polyprenyl synthetase family protein -
  NC849_RS08935 (NC849_08870) rplU 1705633..1705941 (+) 309 WP_003691838.1 50S ribosomal protein L21 -
  NC849_RS08940 (NC849_08875) rpmA 1705966..1706238 (+) 273 WP_003689821.1 50S ribosomal protein L27 -
  NC849_RS08945 (NC849_08880) ubiM 1706473..1707657 (+) 1185 WP_020996717.1 5-demethoxyubiquinol-8 5-hydroxylase UbiM -
  NC849_RS08950 (NC849_08885) rpmG 1707846..1708001 (-) 156 WP_003689824.1 50S ribosomal protein L33 -
  NC849_RS08955 (NC849_08890) rpmB 1708033..1708266 (-) 234 WP_002216391.1 50S ribosomal protein L28 -

Sequence

Protein


Download         Length: 558 a.a.        Molecular weight: 61949.09 Da        Isoelectric Point: 5.5452

>NTDB_id=695746 NC849_RS08915 WP_003703172.1 1701971..1703647(-) (pilF) [Neisseria gonorrhoeae strain 9460]
MSVGLLRILVQNQVVTVERAEHYYNESQAGKEVLPMLFSDGVISPKSLAALIARVFSYSILDLRHYPRHRVLMGVLTEEQ
MVEFHCVPVFRRGDKVFFAVSDPTQMPQIQKTVSAAGIAVELVIVEDDQLAGLLDWVGSRSTSLLQELGEGQEEEESHTL
YIDNEEAEDGPVPRFIHKTLSDALRSGASDIHFEFYEHNARIRFRVDGQLREVVQPPIAVRGQLASRIKVMSRLDISEKR
IPQDGRMQLTFQKGGKPVDFRVSTLPTLFGEKVVMRILNSDAASLNIDQLGFEPFQKKLLLEAIHRPYGMVLVTGPTGSG
KTVSLYTCLNILNTESVNIATAEDPAEINLPGINQVNVNDKQGLTFAAALKSFLRQDPDIIMVGEIRDLETADIAIKAAQ
TGHMVFSTLHTNNAPATLSRMLNMGVAPFNIASSVSLIMAQRLLRRLCSSCKQEVERPSASALKEVGFTDEDLAKDWKLY
RAVGCDRCRGQGYKGRAGVYEVMPISEEMQRVIMNNGTEVDILDVAYKEGMVDLRRAGILKIMQGITSLEEVTANTND

Nucleotide


Download         Length: 1677 bp        

>NTDB_id=695746 NC849_RS08915 WP_003703172.1 1701971..1703647(-) (pilF) [Neisseria gonorrhoeae strain 9460]
ATGAGCGTAGGTTTGCTGAGGATTCTGGTTCAAAACCAGGTGGTTACTGTTGAGCGGGCCGAGCATTACTACAATGAGTC
GCAGGCGGGTAAGGAAGTGTTGCCGATGCTGTTTTCAGACGGTGTCATTTCGCCCAAGTCGCTTGCGGCATTGATTGCGA
GGGTGTTCAGTTATTCGATTCTTGATTTGCGTCATTATCCGCGCCACAGGGTGCTGATGGGGGTGTTGACGGAGGAGCAG
ATGGTGGAGTTCCACTGTGTGCCGGTTTTCCGTCGGGGCGACAAAGTATTTTTTGCGGTTTCCGATCCGACCCAGATGCC
GCAAATTCAGAAAACCGTTTCTGCCGCAGGGATTGCGGTTGAGTTGGTCATTGTCGAGGATGACCAGTTGGCGGGTTTGC
TCGATTGGGTGGGTTCGCGTTCGACATCGCTGCTTCAGGAGCTTGGGGAGGGGCAAGAGGAAGAGGAAAGCCACACCCTG
TATATCGACAACGAGGAGGCAGAAGACGGCCCTGTTCCGAGGTTTATCCATAAAACTTTGTCGGATGCCTTGCGTAGCGG
GGCATCCGACATCCATTTCGAGTTTTACGAACACAATGCGCGTATCCGTTTCCGTGTGGACGGGCAGCTCCGCGAGGTGG
TTCAGCCGCCCATTGCGGTAAGGGGGCAGCTTGCTTCCCGGATTAAGGTAATGTCGCGTTTGGACATTTCCGAAAAACGG
ATACCGCAGGACGGTAGGATGCAGCTGACCTTTCAAAAGGGCGGCAAGCCTGTCGATTTCCGTGTCAGCACATTGCCGAC
GCTGTTTGGCGAAAAGGTCGTGATGCGGATTTTGAATTCCGATGCCGCGTCTTTGAACATCGACCAGCTCGGTTTTGAGC
CGTTCCAGAAAAAATTGTTGTTGGAAGCGATTCACCGTCCTTACGGGATGGTGCTGGTAACCGGCCCGACGGGTTCGGGT
AAGACGGTGTCGCTCTATACCTGTTTGAATATTTTGAATACGGAGTCGGTAAATATTGCAACGGCGGAAGACCCTGCCGA
GATTAACCTGCCGGGCATCAATCAGGTTAACGTCAATGATAAGCAGGGTCTGACTTTTGCCGCTGCTTTGAAGTCTTTCC
TGCGTCAGGACCCGGACATCATTATGGTCGGTGAGATTCGTGATTTGGAAACTGCCGATATTGCGATTAAGGCGGCACAA
ACAGGGCATATGGTGTTTTCCACACTGCACACGAATAATGCGCCGGCGACGTTGTCGCGTATGCTGAATATGGGTGTCGC
GCCGTTTAATATTGCCAGTTCGGTCAGCCTGATTATGGCGCAGCGTCTTTTACGCAGGCTGTGTTCGAGCTGCAAACAGG
AAGTGGAACGCCCGTCTGCCTCTGCTTTGAAGGAAGTCGGTTTCACCGATGAGGATCTTGCAAAAGATTGGAAACTTTAC
CGCGCCGTCGGTTGCGACCGTTGCCGGGGGCAGGGTTATAAGGGGCGTGCGGGCGTGTATGAGGTTATGCCCATCAGCGA
AGAAATGCAGCGTGTGATTATGAACAACGGTACGGAAGTGGATATTTTGGACGTTGCCTATAAGGAGGGTATGGTGGATT
TGCGCCGGGCCGGTATTTTGAAAATTATGCAGGGCATTACTTCATTGGAAGAGGTAACGGCAAATACCAACGATTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(38-139)

Type II/IV secretion system protein (T2SSE)

(178-446)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

99.642

100

0.996
  pilB Acinetobacter baumannii D1279779

50.088

100

0.509
  pilB Acinetobacter baylyi ADP1

50.178

100

0.505
  pilB Legionella pneumophila strain ERS1305867

47.35

100

0.48
  pilB Vibrio parahaemolyticus RIMD 2210633

45.213

100

0.457
  pilB Vibrio cholerae strain A1552

45.196

100

0.455
  pilB Vibrio campbellii strain DS40M4

43.662

100

0.444
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.885

93.907

0.375