Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   NCL52_RS12830 Genome accession   NZ_CP098417
Coordinates   2505376..2505993 (-) Length   205 a.a.
NCBI ID   WP_137200853.1    Uniprot ID   -
Organism   Bacillus subtilis strain N2-10     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2500376..2510993
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NCL52_RS12800 (NCL52_12800) rpsT 2500678..2500944 (+) 267 WP_003229989.1 30S ribosomal protein S20 -
  NCL52_RS12805 (NCL52_12805) holA 2500959..2502002 (-) 1044 WP_015714286.1 DNA polymerase III subunit delta -
  NCL52_RS12810 (NCL52_12810) - 2502042..2502191 (-) 150 WP_120363468.1 hypothetical protein -
  NCL52_RS12815 (NCL52_12815) yqzM 2502232..2502366 (+) 135 WP_003229983.1 YqzM family protein -
  NCL52_RS12820 (NCL52_12820) comEC 2502406..2504736 (-) 2331 WP_137200851.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  NCL52_RS12825 (NCL52_12825) comEB 2504740..2505309 (-) 570 WP_003229978.1 ComE operon protein 2 -
  NCL52_RS12830 (NCL52_12830) comEA 2505376..2505993 (-) 618 WP_137200853.1 competence protein ComEA Machinery gene
  NCL52_RS12835 (NCL52_12835) comER 2506077..2506898 (+) 822 WP_134981848.1 late competence protein ComER -
  NCL52_RS12840 (NCL52_12840) yqeM 2506964..2507707 (-) 744 WP_033884459.1 class I SAM-dependent methyltransferase -
  NCL52_RS12845 (NCL52_12845) rsfS 2507704..2508060 (-) 357 WP_014480315.1 ribosome silencing factor -
  NCL52_RS12850 (NCL52_12850) yqeK 2508078..2508638 (-) 561 WP_014480316.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  NCL52_RS12855 (NCL52_12855) nadD 2508628..2509197 (-) 570 WP_004398676.1 nicotinate-nucleotide adenylyltransferase -
  NCL52_RS12860 (NCL52_12860) yhbY 2509209..2509499 (-) 291 WP_003226133.1 ribosome assembly RNA-binding protein YhbY -
  NCL52_RS12865 (NCL52_12865) aroE 2509493..2510335 (-) 843 WP_003229967.1 shikimate dehydrogenase -

Sequence

Protein


Download         Length: 205 a.a.        Molecular weight: 21786.48 Da        Isoelectric Point: 4.7284

>NTDB_id=695394 NCL52_RS12830 WP_137200853.1 2505376..2505993(-) (comEA) [Bacillus subtilis strain N2-10]
MNWLNQHKKTIILAASAAVFTAIMIFLATGKNKEPVKQAVPTETENTVVKQEANNDESSETIVIDIKGAVQHPGVYEMRT
GDRVSQAIEKAGGTSEQADEAQVNLAEILQDGTVVYIPKKGEETAVQQGGGGSVQSDGGKGALVNINTATLEELQGISGV
GPSKAEAIIAYREENGRFQTIEEITKVSGIGEKSFEKIKSSITVK

Nucleotide


Download         Length: 618 bp        

>NTDB_id=695394 NCL52_RS12830 WP_137200853.1 2505376..2505993(-) (comEA) [Bacillus subtilis strain N2-10]
ATGAATTGGTTGAATCAGCATAAGAAAACAATTATTTTAGCGGCTTCTGCGGCTGTTTTCACAGCGATTATGATCTTTCT
GGCCACAGGGAAAAATAAAGAGCCGGTGAAGCAAGCTGTACCAACAGAGACAGAAAATACAGTGGTAAAGCAGGAAGCAA
ACAACGACGAGTCAAGCGAAACAATTGTGATAGACATCAAAGGTGCTGTTCAGCATCCTGGCGTTTATGAAATGCGAACA
GGGGACAGAGTATCTCAGGCAATTGAGAAAGCGGGCGGGACCAGTGAACAAGCAGACGAAGCGCAAGTAAATTTGGCGGA
GATTTTGCAGGACGGGACAGTGGTGTACATCCCGAAAAAGGGAGAGGAAACAGCAGTGCAGCAAGGTGGCGGAGGGTCTG
TCCAAAGCGATGGAGGGAAGGGAGCGCTGGTGAATATCAATACAGCAACCTTAGAGGAGTTACAAGGCATCTCAGGGGTG
GGGCCATCCAAAGCTGAAGCTATTATTGCATACCGGGAAGAAAACGGGCGTTTCCAAACAATTGAAGAAATCACTAAGGT
TTCAGGAATAGGTGAAAAGTCATTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(142-203)

SLBB domain (SLBB)

(63-118)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

98.537

100

0.985
  comEA Staphylococcus aureus MW2

36.818

100

0.395
  comEA Staphylococcus aureus N315

35.455

100

0.38