Detailed information    

insolico Bioinformatically predicted

Overview

Name   comE   Type   Machinery gene
Locus tag   PCK13_RS16895 Genome accession   NZ_CP115178
Coordinates   3436967..3438205 (-) Length   412 a.a.
NCBI ID   WP_000815968.1    Uniprot ID   -
Organism   Escherichia coli strain B     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3431967..3443205
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PCK13_RS16875 (PCK13_16865) dam 3432579..3433415 (-) 837 WP_000742143.1 adenine-specific DNA-methyltransferase -
  PCK13_RS16880 (PCK13_16870) damX 3433522..3434808 (-) 1287 WP_000343193.1 cell division protein DamX -
  PCK13_RS16885 (PCK13_16875) aroB 3434900..3435988 (-) 1089 WP_000439846.1 3-dehydroquinate synthase -
  PCK13_RS16890 (PCK13_16880) aroK 3436045..3436566 (-) 522 WP_000818618.1 shikimate kinase AroK -
  PCK13_RS16895 (PCK13_16885) comE 3436967..3438205 (-) 1239 WP_000815968.1 DNA uptake porin HofQ Machinery gene
  PCK13_RS16900 (PCK13_16890) hofP 3438117..3438521 (-) 405 WP_001300917.1 DNA utilization protein HofP -
  PCK13_RS16905 (PCK13_16895) hofO 3438511..3438951 (-) 441 WP_001055763.1 DNA utilization protein HofO -
  PCK13_RS16910 (PCK13_16900) hofN 3438935..3439474 (-) 540 WP_001069315.1 DNA utilization protein HofN -
  PCK13_RS16915 (PCK13_16905) hofM 3439474..3440253 (-) 780 WP_001297394.1 DNA utilization protein HofM -
  PCK13_RS16920 (PCK13_16910) mrcA 3440373..3442925 (+) 2553 WP_000673804.1 peptidoglycan glycosyltransferase/peptidoglycan DD-transpeptidase MrcA -

Sequence

Protein


Download         Length: 412 a.a.        Molecular weight: 44774.27 Da        Isoelectric Point: 6.1173

>NTDB_id=694255 PCK13_RS16895 WP_000815968.1 3436967..3438205(-) (comE) [Escherichia coli strain B]
MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQEKLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGL
ITRQEDNILSVHSIAWQNNNIARQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTTLGSDLSVATATTHVGFNIGRIN
GRLLDLELSALEQKQQLDIIASPRLLASHLQPASIKQGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKL
HISQNVPGQVLQQADGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDGKEDERREL
VVFITPRLVSSE

Nucleotide


Download         Length: 1239 bp        

>NTDB_id=694255 PCK13_RS16895 WP_000815968.1 3436967..3438205(-) (comE) [Escherichia coli strain B]
ATGAAGCAATGGATAGCCGCACTACTGTTGATGCTGATACCCGGCGTACAGGCGGCAAAGCCGCAAAAAGTGACGCTGAT
GGTGGATGACGTTCCGGTAGCTCAGGTGTTGCAGGCGCTGGCTGAACAGGAGAAGTTGAACCTGGTCGTGTCGCCAGACG
TCAGCGGTACGGTGTCGTTACATCTAACAGATGTTCCCTGGAAGCAGGCACTACAAACTGTAGTGAAAAGCGCCGGACTG
ATAACGCGGCAGGAAGACAACATTCTCTCAGTGCATTCCATTGCCTGGCAGAATAACAATATCGCCCGCCAGGAGGCGGA
GCAGGCGCGGGCGCAGGCAAATCTGCCGCTGGAAAATCGCAGTATAACCCTGCAATACGCCGACGCGGGAGAACTGGCGA
AAGCGGGGGAGAAGCTACTGAGTGCCAAAGGGAGTATGACCGTCGATAAACGCACCAATCGCCTTTTGCTACGAGATAAC
AAAACGGCGTTAAGCGCGCTTGAACAGTGGGTAGCGCAAATGGATCTGCCGGTCGGGCAGGTTGAGCTGTCGGCGCATAT
TGTCACCATTAATGAAAAAAGTTTGCGTGAGTTAGGCGTGAAATGGACGCTGGCCGATGCGCAACACGCTGGTGGCGTTG
GGCAAGTCACCACGCTTGGTAGCGACCTCTCCGTAGCGACGGCGACAACGCATGTCGGTTTTAACATTGGGCGCATCAAC
GGACGCTTGCTGGATCTTGAGCTTTCTGCGCTCGAACAAAAACAGCAGCTGGATATTATCGCCAGTCCGCGTCTGCTGGC
CTCACATCTTCAGCCTGCCAGCATTAAACAGGGGAGCGAAATTCCATATCAGGTTTCCAGCGGGGAAAGTGGCGCGACGT
CGGTGGAATTTAAAGAGGCCGTCCTGGGGATGGAGGTCACGCCCACGGTGTTACAAAAAGGTCGCATCCGGCTGAAATTA
CACATCAGCCAGAACGTTCCGGGGCAGGTGCTACAGCAGGCCGATGGCGAAGTGCTGGCGATTGATAAGCAGGAGATCGA
AACGCAGGTCGAGGTCAAAAGCGGAGAAACGTTGGCGCTGGGCGGCATTTTTACCCGTAAAAATAAATCGGGTCAGGATA
GCGTACCGTTGCTTGGCGACATTCCCTGGTTCGGGCAATTATTTCGTCATGACGGAAAAGAAGATGAACGACGCGAGTTA
GTGGTGTTTATCACGCCACGACTGGTTTCCAGTGAGTAA

Domains

Predicted by InterProScan.

Bacterial type II and III secretion system protein (Secretin)

(250-409)

Bacterial type II/III secretion system short domain (Secretin_N)

(120-179)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comE Haemophilus influenzae Rd KW20

37.385

100

0.396
  comE Haemophilus influenzae 86-028NP

37.156

100

0.393
  pilQ Vibrio campbellii strain DS40M4

37.767

100

0.386
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

37.288

100

0.374
  pilQ Vibrio cholerae strain A1552

37.288

100

0.374
  comE Glaesserella parasuis strain SC1401

35.952

100

0.367