Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   M8T91_RS00730 Genome accession   NZ_CP098023
Coordinates   162772..163848 (+) Length   358 a.a.
NCBI ID   WP_301415839.1    Uniprot ID   -
Organism   Microbulbifer sp. MI-G     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 157772..168848
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M8T91_RS00720 (M8T91_00720) - 159132..159614 (+) 483 WP_301415835.1 chemotaxis protein CheW -
  M8T91_RS00725 (M8T91_00725) - 159967..162624 (-) 2658 WP_301415837.1 penicillin-binding protein 1A -
  M8T91_RS00730 (M8T91_00730) pilM 162772..163848 (+) 1077 WP_301415839.1 pilus assembly protein PilM Machinery gene
  M8T91_RS00735 (M8T91_00735) - 163850..164425 (+) 576 WP_301415841.1 PilN domain-containing protein -
  M8T91_RS00740 (M8T91_00740) - 164438..165058 (+) 621 WP_301415843.1 type 4a pilus biogenesis protein PilO -
  M8T91_RS00745 (M8T91_00745) - 165055..165585 (+) 531 WP_301415845.1 pilus assembly protein PilP -
  M8T91_RS00750 (M8T91_00750) pilQ 165675..167768 (+) 2094 WP_301415847.1 type IV pilus secretin PilQ -
  M8T91_RS00755 (M8T91_00755) aroK 167768..168289 (+) 522 WP_301419163.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 358 a.a.        Molecular weight: 38791.07 Da        Isoelectric Point: 4.4067

>NTDB_id=693580 M8T91_RS00730 WP_301415839.1 162772..163848(+) (pilM) [Microbulbifer sp. MI-G]
MGIFPFLNKGQKAMLGLDISSTAVKLLELSRNGDKYRVEGYAVEPLPPNAVVDKNINDIGAAAEAIRKVVRRSKTRLRQA
AVAVSGSAVITKTLEMPADLSDEDLEARIALEADQYIPYPLEEVSLDFEVQGPSEKGDDQVEVLLAACRSENIDQRVSAL
AEAELEAGVVDVEAYAIERIYTLLEGQLPPQEQLVAAVVDIGATMSALSILVDGKTVYTREQLFGGRQLTDEIQRRYGLS
SEEAALAKHQGGSGLPDDYHTEVLEPFRDAVIQQITRSLQFFYSSTSYSDVDYILLCGGVAAMEGLSELVGEKLGKPTLI
ANPFAGMSISSRVNHKALGREAPALLIAAGLAMREKEY

Nucleotide


Download         Length: 1077 bp        

>NTDB_id=693580 M8T91_RS00730 WP_301415839.1 162772..163848(+) (pilM) [Microbulbifer sp. MI-G]
ATGGGGATTTTCCCTTTTCTGAATAAAGGCCAAAAGGCCATGCTGGGGCTCGATATTAGCTCTACTGCGGTGAAATTGCT
GGAGCTGAGTCGCAATGGTGATAAATATCGAGTGGAGGGTTACGCCGTGGAGCCCCTGCCTCCCAATGCGGTGGTGGACA
AAAACATCAACGATATCGGGGCTGCCGCTGAGGCTATCCGCAAGGTGGTGCGCCGATCCAAAACCCGCCTGCGTCAAGCC
GCCGTGGCCGTTTCCGGTTCGGCGGTGATTACCAAAACGCTGGAAATGCCCGCGGATCTCTCCGATGAAGACTTGGAGGC
GCGGATTGCACTGGAGGCAGACCAGTATATTCCCTACCCCCTCGAAGAAGTCAGTCTGGATTTCGAAGTGCAGGGTCCTT
CTGAGAAAGGCGATGATCAGGTGGAGGTTCTGCTGGCAGCATGCCGAAGCGAAAACATTGATCAGCGCGTTTCCGCGCTT
GCAGAAGCCGAATTGGAAGCCGGTGTGGTTGATGTGGAAGCCTATGCCATAGAGCGTATATATACTCTTTTGGAAGGACA
ATTGCCACCACAGGAGCAACTGGTTGCCGCTGTTGTGGATATTGGCGCCACTATGAGTGCTCTGTCCATTTTGGTGGATG
GCAAGACCGTTTATACCCGTGAGCAACTTTTCGGTGGGCGTCAACTTACCGATGAAATACAGCGCCGTTACGGCCTGTCA
TCGGAAGAGGCTGCCCTGGCGAAACACCAGGGCGGTAGCGGACTGCCGGACGATTATCACACTGAGGTGCTGGAACCTTT
CCGCGACGCGGTGATTCAGCAAATCACCCGTTCTCTGCAGTTCTTTTACTCTTCAACTTCCTACAGTGATGTCGATTACA
TCCTGCTCTGCGGCGGTGTTGCGGCCATGGAAGGCTTGTCTGAACTGGTTGGCGAGAAACTGGGCAAGCCCACGTTAATC
GCTAACCCGTTTGCGGGGATGAGTATCTCCTCCAGGGTCAATCACAAGGCGCTGGGCAGAGAGGCTCCGGCCCTGCTGAT
TGCCGCAGGCCTGGCGATGCGGGAAAAGGAATACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(16-355)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

49.711

96.648

0.48
  comM Acinetobacter nosocomialis M2

49.711

96.648

0.48
  comM Acinetobacter baylyi ADP1

46.821

96.648

0.453
  pilM Legionella pneumophila strain ERS1305867

42.978

99.441

0.427