Detailed information NTG0693199

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: NAG75_RS03870
• Genome accession: NZ_CP097873
• Coordinates: 630348..631217 (-)
• Length: 289 a.a.
• NCBI ID: WP_005493974.1
• Uniprot ID: A0A249W3M2
• Organism: Vibrio parahaemolyticus strain GL-601
• Function: type IV pilus biogenesis and function (predicted from homology) DNA binding and uptake

Genomic Context

Location: 625348..636217

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
NAG75_RS03835	rpsP	626389..626637 (+)	249	WP_005379962.1	30S ribosomal protein S16	-
NAG75_RS03840	rimM	626666..627214 (+)	549	WP_005462552.1	ribosome maturation factor RimM	-
NAG75_RS03845	trmD	627242..627985 (+)	744	WP_005462562.1	tRNA (guanosine(37)-N1)-methyltransferase TrmD	-
NAG75_RS03850	rplS	628027..628380 (+)	354	WP_005462554.1	50S ribosomal protein L19	-
NAG75_RS03855	yacG	628702..628896 (-)	195	WP_005462546.1	DNA gyrase inhibitor YacG	-
NAG75_RS03860	zapD	628965..629705 (-)	741	WP_015297254.1	cell division protein ZapD	-
NAG75_RS03865	coaE	629733..630347 (-)	615	WP_005480887.1	dephospho-CoA kinase	-
NAG75_RS03870	pilD	630348..631217 (-)	870	WP_005493974.1	A24 family peptidase	Machinery gene
NAG75_RS03875	pilC	631282..632505 (-)	1224	WP_021822433.1	type II secretion system F family protein	Machinery gene
NAG75_RS03880	pilB	632529..634214 (-)	1686	WP_080607318.1	type IV-A pilus assembly ATPase PilB	Machinery gene
NAG75_RS03885	-	634216..634668 (-)	453	WP_080607317.1	prepilin-type N-terminal cleavage/methylation domain-containing protein	-
NAG75_RS03890	nadC	634932..635819 (-)	888	WP_208893453.1	carboxylating nicotinate-nucleotide diphosphorylase	-

Sequence

Protein

• Length: 289 a.a.
• Molecular weight: 32377.43 Da
• Isoelectric Point: 7.1971

>NTDB_id=693199 NAG75_RS03870 WP_005493974.1 630348..631217(-) (pilD) [Vibrio parahaemolyticus strain GL-601]
MEVFQYYPWLFVVFASIFGLIVGSFLNVVIYRLPKIMELEWRRECAESFPEYKIKPPQEVLTLSVPRSSCQNCATPIRIR
DNIPVISWLLLKGKCHHCHTAISPRYPLIELLTAACAGFVAYHFGFSYFTVALIFFTFVLIAATFIDLDTMLLPDQLTLP
LTWAGIALALTEISPVSLQDAVIGAIAGYLCLWSVYWGFKLLTGKEGMGYGDFKLLAALGAWLGWQSLPMIILLSSVVGV
IFGLVQLRLQKQGIERAFPFGPYLAIAGWASLIWGDQILSWYFTSILGV

Nucleotide

• Length: 870 bp

>NTDB_id=693199 NAG75_RS03870 WP_005493974.1 630348..631217(-) (pilD) [Vibrio parahaemolyticus strain GL-601]
ATGGAAGTGTTCCAGTACTACCCTTGGCTATTCGTTGTGTTTGCCAGTATCTTCGGTTTAATTGTGGGTAGTTTTCTTAA
TGTGGTCATTTATCGGCTACCTAAAATCATGGAATTAGAATGGCGCCGTGAATGCGCCGAATCATTCCCTGAATATAAGA
TTAAGCCACCTCAAGAAGTTTTGACATTGAGCGTGCCTCGCTCTTCGTGCCAAAACTGCGCGACGCCAATTCGTATTCGA
GACAATATTCCAGTAATCAGCTGGCTATTGTTAAAAGGAAAATGCCATCATTGTCATACCGCAATTAGTCCTCGCTATCC
GCTGATAGAATTACTCACTGCAGCGTGCGCTGGTTTTGTGGCTTATCATTTCGGCTTTAGCTACTTTACGGTTGCGTTAA
TCTTTTTCACATTTGTCTTAATAGCCGCGACTTTTATCGATCTCGATACTATGCTGCTACCGGACCAATTAACATTACCA
TTAACTTGGGCTGGCATTGCGTTAGCATTAACAGAAATAAGCCCTGTTAGTCTACAAGACGCCGTAATTGGTGCGATTGC
CGGTTATTTGTGTCTTTGGTCAGTATATTGGGGTTTCAAACTGCTTACTGGTAAAGAAGGCATGGGTTACGGTGATTTTA
AATTGCTGGCTGCCCTTGGCGCTTGGCTAGGATGGCAATCATTACCAATGATCATCCTACTTTCTTCCGTCGTCGGTGTG
ATATTCGGGCTCGTTCAGTTACGACTACAAAAACAAGGTATAGAGAGAGCTTTCCCTTTCGGCCCATATTTAGCGATTGC
AGGATGGGCAAGCTTAATCTGGGGCGATCAAATTCTAAGTTGGTACTTCACGTCAATTTTAGGAGTGTAA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (135-244)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (17-124)

3D structure

• AlphaFold DB: A0A249W3M2

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio campbellii strain DS40M4	88.927	100	0.889
pilD	Vibrio cholerae strain A1552	73.611	99.654	0.734
pilD	Acinetobacter baumannii D1279779	52.239	92.734	0.484
pilD	Acinetobacter nosocomialis M2	51.866	92.734	0.481
pilD	Neisseria gonorrhoeae MS11	47.101	95.502	0.45