Detailed information NTG0693179

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: LpnA194_RS07345
• Genome accession: NZ_CP114578
• Coordinates: 1616963..1617826 (+)
• Length: 287 a.a.
• NCBI ID: WP_027219447.1
• Uniprot ID: I7HPT9
• Organism: Legionella pneumophila strain A194
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1611963..1622826

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LpnA194_RS07325 (LpnA194_01468)	-	1612444..1612770 (-)	327	WP_027219451.1	DUF1820 family protein	-
LpnA194_RS07330 (LpnA194_01469)	-	1612953..1613696 (-)	744	WP_027219450.1	methyltransferase domain-containing protein	-
LpnA194_RS07335 (LpnA194_01470)	pilB	1613967..1615691 (+)	1725	WP_027219449.1	type IV-A pilus assembly ATPase PilB	Machinery gene
LpnA194_RS07340 (LpnA194_01471)	pilC	1615697..1616917 (+)	1221	WP_027219448.1	type II secretion system F family protein	Machinery gene
LpnA194_RS07345 (LpnA194_01472)	pilD	1616963..1617826 (+)	864	WP_027219447.1	prepilin peptidase	Machinery gene
LpnA194_RS07350 (LpnA194_01473)	-	1617840..1619045 (-)	1206	WP_040147307.1	cyclic nucleotide-binding domain-containing protein	-
LpnA194_RS07355 (LpnA194_01474)	-	1619153..1619746 (-)	594	WP_011946528.1	hypothetical protein	-
LpnA194_RS07360 (LpnA194_01475)	-	1619762..1620217 (-)	456	WP_011946529.1	hypothetical protein	-
LpnA194_RS07365 (LpnA194_01476)	-	1620351..1621166 (+)	816	WP_010947257.1	pyruvate, water dikinase regulatory protein	-
LpnA194_RS07370 (LpnA194_01477)	-	1621286..1622734 (-)	1449	WP_027220101.1	bifunctional 2-methylcitrate dehydratase/aconitate hydratase	-

Sequence

Protein

• Length: 287 a.a.
• Molecular weight: 32832.42 Da
• Isoelectric Point: 7.8427

>NTDB_id=693179 LpnA194_RS07345 WP_027219447.1 1616963..1617826(+) (pilD) [Legionella pneumophila strain A194]
MINALIINYPWFMYLVVGLFSLAVGSLLNVIIYRLPIILQEEWKEQCCELFHLEQQKEKIKLNLFLPRSFCPHCKAMVKA
WQNIPLLSYILLRGRCYQCNSPISIRYPFVELLTTVLSVYASWHFGFTIQLLFALLAIWILIPLVYIDLDHQLLPDSLTL
GFLWLGLIANTQNVFVSLDVAVLSCAGAYLALWLFINLFYLMTGKVGMGHGDFKLFAAFGAWFGWMYLPLILLISSITGA
IIGLIYLKINGKSRDTAIPFGPFLCISGLIAMFWGDSIINWYIGYWM

Nucleotide

• Length: 864 bp

>NTDB_id=693179 LpnA194_RS07345 WP_027219447.1 1616963..1617826(+) (pilD) [Legionella pneumophila strain A194]
ATGATAAATGCACTAATAATCAATTATCCATGGTTTATGTATTTAGTCGTTGGACTCTTTTCTCTAGCCGTTGGAAGTTT
ACTTAATGTGATAATCTATCGCTTACCGATTATTTTGCAGGAAGAGTGGAAAGAGCAATGCTGTGAGCTATTCCATCTCG
AACAGCAAAAAGAAAAAATAAAACTTAATCTATTTTTACCACGTTCTTTTTGTCCTCATTGTAAAGCGATGGTTAAAGCG
TGGCAGAATATTCCCCTGTTAAGCTATATTTTATTAAGAGGACGCTGTTACCAATGTAATAGTCCAATTTCAATCCGCTA
CCCTTTTGTTGAGCTGTTGACAACAGTTTTATCCGTATACGCCAGTTGGCATTTTGGGTTTACAATACAATTATTATTTG
CTTTATTAGCCATCTGGATATTAATACCACTTGTTTATATTGATCTTGATCATCAATTACTCCCTGATAGCCTCACTTTA
GGGTTTTTATGGCTTGGCCTTATTGCTAATACCCAGAACGTATTTGTTAGTCTTGATGTTGCTGTATTAAGCTGCGCTGG
CGCCTATTTAGCCTTATGGCTATTCATTAATCTGTTTTATCTGATGACTGGTAAAGTAGGTATGGGACATGGAGATTTCA
AATTATTTGCGGCATTTGGCGCATGGTTTGGATGGATGTATTTACCATTAATTCTTCTAATATCTTCAATAACCGGGGCT
ATTATTGGTTTGATTTATTTAAAGATAAATGGTAAATCAAGAGATACTGCAATACCATTCGGTCCTTTTCTTTGCATTTC
TGGATTAATAGCTATGTTTTGGGGAGACTCCATTATCAATTGGTATATTGGTTATTGGATGTAA

Domains

Predicted by InterProScan.

• Type IV leader peptidase family (Peptidase_A24) (136-244)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (19-125)

3D structure

• AlphaFold DB: I7HPT9

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	48.043	97.909	0.47
pilD	Acinetobacter nosocomialis M2	47.619	95.122	0.453
pilD	Acinetobacter baumannii D1279779	46.909	95.819	0.449
pilD	Vibrio campbellii strain DS40M4	44.604	96.864	0.432
pilD	Neisseria gonorrhoeae MS11	42.086	96.864	0.408