Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   M9420_RS06180 Genome accession   NZ_CP097796
Coordinates   1295184..1295618 (+) Length   144 a.a.
NCBI ID   WP_014668227.1    Uniprot ID   -
Organism   Pasteurella multocida strain 40540     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1290184..1300618
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M9420_RS06150 (M9420_06150) tadA 1290293..1290781 (-) 489 WP_016534447.1 tRNA adenosine(34) deaminase TadA -
  M9420_RS06155 (M9420_06155) - 1290808..1291659 (-) 852 WP_014668231.1 thymidylate synthase -
  M9420_RS06160 (M9420_06160) lgt 1291665..1292468 (-) 804 WP_014668230.1 prolipoprotein diacylglyceryl transferase -
  M9420_RS06165 (M9420_06165) - 1292478..1293272 (-) 795 WP_005719128.1 sulfite exporter TauE/SafE family protein -
  M9420_RS06170 (M9420_06170) rppH 1293275..1293871 (-) 597 WP_014668229.1 RNA pyrophosphohydrolase -
  M9420_RS06175 (M9420_06175) ampD 1294457..1295023 (-) 567 WP_014668228.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  M9420_RS06180 (M9420_06180) pilA 1295184..1295618 (+) 435 WP_014668227.1 prepilin peptidase-dependent pilin Machinery gene
  M9420_RS06185 (M9420_06185) pilB 1295618..1297003 (+) 1386 WP_014668226.1 GspE/PulE family protein Machinery gene
  M9420_RS06190 (M9420_06190) - 1297003..1298214 (+) 1212 WP_014668225.1 type II secretion system F family protein -
  M9420_RS06195 (M9420_06195) - 1298214..1298936 (+) 723 WP_016534448.1 A24 family peptidase -
  M9420_RS06200 (M9420_06200) coaE 1298951..1299571 (+) 621 WP_005751078.1 dephospho-CoA kinase -
  M9420_RS06205 (M9420_06205) yacG 1299582..1299785 (+) 204 WP_005719879.1 DNA gyrase inhibitor YacG -
  M9420_RS06210 (M9420_06210) - 1299785..1300054 (+) 270 WP_005722568.1 GNAT family N-acetyltransferase -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 15084.52 Da        Isoelectric Point: 9.1281

>NTDB_id=692624 M9420_RS06180 WP_014668227.1 1295184..1295618(+) (pilA) [Pasteurella multocida strain 40540]
MKKAIFFSIKKGFTLIELMIVIAIIAILATIAVPSYQNYTKKAAISELLQAAAPYRAEVELCIYNTNSTTNCNAGSHGIR
ADAAASKKYLKAITVKAGVITVTGQGSLEGISYTLTPSGNAAQGVSWTVNCGAQSDIFPAGFCA

Nucleotide


Download         Length: 435 bp        

>NTDB_id=692624 M9420_RS06180 WP_014668227.1 1295184..1295618(+) (pilA) [Pasteurella multocida strain 40540]
ATGAAAAAAGCCATTTTCTTTTCGATTAAAAAAGGGTTTACGCTAATTGAATTAATGATTGTCATTGCCATTATTGCGAT
TTTAGCCACGATTGCCGTGCCATCTTATCAAAATTATACAAAAAAAGCGGCAATCTCTGAATTATTGCAAGCCGCTGCTC
CTTATCGTGCCGAGGTAGAACTTTGTATCTATAATACAAATAGTACAACGAATTGTAATGCAGGAAGTCATGGTATTCGT
GCTGATGCTGCAGCAAGTAAAAAATATTTAAAAGCCATCACGGTTAAAGCTGGGGTAATTACTGTCACTGGACAAGGGAG
CTTGGAAGGAATTAGCTATACGCTGACACCGAGTGGTAACGCTGCTCAAGGTGTTTCTTGGACAGTTAATTGTGGTGCTC
AATCTGACATTTTCCCAGCAGGATTTTGTGCATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(10-32)

Pilin (bacterial filament) (Pilin)

(42-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

62.5

100

0.625
  pilA Haemophilus influenzae Rd KW20

60.417

100

0.604
  pilA Glaesserella parasuis strain SC1401

53.02

100

0.549
  pilA/pilA1 Eikenella corrodens VA1

38.129

96.528

0.368