Detailed information    

insolico Bioinformatically predicted

Overview

Name   comE   Type   Machinery gene
Locus tag   M9414_RS08995 Genome accession   NZ_CP097795
Coordinates   1917126..1918460 (-) Length   444 a.a.
NCBI ID   WP_250269275.1    Uniprot ID   -
Organism   Pasteurella multocida strain 34030     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1912126..1923460
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M9414_RS08970 (M9414_08970) secM 1913645..1913959 (-) 315 WP_010907080.1 secA translation cis-regulator SecM -
  M9414_RS08975 (M9414_08975) - 1914049..1914354 (+) 306 WP_083003370.1 DUF721 domain-containing protein -
  M9414_RS08980 (M9414_08980) - 1914402..1915307 (-) 906 WP_083003372.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -
  M9414_RS08985 (M9414_08985) aroB 1915311..1916399 (-) 1089 WP_115098218.1 3-dehydroquinate synthase -
  M9414_RS08990 (M9414_08990) aroK 1916416..1916943 (-) 528 WP_005717623.1 shikimate kinase AroK -
  M9414_RS08995 (M9414_08995) comE 1917126..1918460 (-) 1335 WP_250269275.1 type IV pilus secretin PilQ Machinery gene
  M9414_RS09000 (M9414_09000) - 1918470..1918832 (-) 363 WP_115300060.1 pilus assembly protein PilP -
  M9414_RS09005 (M9414_09005) - 1918835..1919359 (-) 525 WP_315582728.1 ATPase -
  M9414_RS09010 (M9414_09010) - 1919359..1919874 (-) 516 WP_250269276.1 competence protein ComB -
  M9414_RS09015 (M9414_09015) - 1919914..1920687 (-) 774 WP_083003387.1 pilus assembly protein PilM -
  M9414_RS09020 (M9414_09020) - 1920823..1923384 (+) 2562 WP_250269277.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 444 a.a.        Molecular weight: 49232.61 Da        Isoelectric Point: 7.1842

>NTDB_id=692603 M9414_RS08995 WP_250269275.1 1917126..1918460(-) (comE) [Pasteurella multocida strain 34030]
MWRAFRKLSFVYFLCGVAYVGSSQAQDAEHFYLRLKQAPLVEMLQYLALQQHQDLLIDDHLEGTLSLQMKKTTFEKCLQS
IAKMKQLEFHQEGKSYYLTSPSGVAANDTHHPTSLMTSSIKLHFAKAAEVVKSLTSGQGSLLSVGGSLSFDERTNLLLIQ
DEPQSIQRIKALVAEMDKPIEQIAIEARIVTMTDESLQELGVRWGLFQATEQAHTIAGSLAANGFSNIENQLNVNFTTNS
APVGSIALQLAKINGRLLDLELTALEREKHIEIIASPRLLTTNKKSASIKQGTEIPYVMKRGKDKSESVEFREAVLGLDV
TPHISKDNTILLDLLITQNTLGAPVVYDKGEIVSIDKQEINTQVVAQDGETIVLGGVFHDTMTKGVNKVPLLGDLPLLKH
VFSQETERHQKRELVIFVTPHIIKPTQSLPEQKTTGVKKSAKSR

Nucleotide


Download         Length: 1335 bp        

>NTDB_id=692603 M9414_RS08995 WP_250269275.1 1917126..1918460(-) (comE) [Pasteurella multocida strain 34030]
ATGTGGCGAGCATTCAGAAAACTATCTTTTGTGTACTTTTTATGTGGGGTTGCTTATGTTGGAAGTAGTCAAGCACAAGA
CGCAGAACATTTTTATTTACGTTTAAAACAAGCACCTTTAGTCGAAATGTTACAGTATTTAGCATTGCAACAACATCAGG
ATTTGTTAATCGATGATCATTTAGAGGGCACATTATCATTACAGATGAAAAAGACAACCTTTGAGAAATGTTTACAGTCG
ATTGCAAAAATGAAACAACTTGAATTCCATCAAGAAGGAAAATCCTATTATTTAACTTCCCCTTCAGGTGTTGCGGCAAA
CGATACTCATCATCCTACGTCATTGATGACATCTTCAATAAAATTGCATTTTGCCAAAGCCGCAGAGGTGGTGAAATCTT
TAACTTCAGGGCAGGGAAGTTTACTTTCTGTCGGGGGGAGTTTGAGTTTTGATGAGCGGACTAATTTACTGCTGATTCAG
GATGAACCGCAATCAATACAGCGTATTAAAGCATTAGTAGCAGAAATGGATAAACCCATTGAACAAATTGCGATCGAAGC
CAGGATTGTGACGATGACAGACGAAAGTTTGCAGGAACTTGGTGTAAGATGGGGGCTATTTCAAGCAACAGAACAGGCAC
ATACTATTGCGGGGAGTTTAGCCGCGAACGGCTTTTCGAATATAGAAAACCAATTAAATGTGAATTTTACGACCAATAGT
GCACCTGTTGGTTCCATCGCCTTACAGTTGGCGAAAATAAATGGTCGATTATTAGACTTGGAATTAACTGCCTTAGAGCG
AGAAAAGCATATTGAGATTATTGCGAGTCCTCGTTTATTAACAACGAATAAAAAAAGTGCCAGTATCAAACAAGGGACGG
AAATTCCTTATGTGATGAAACGGGGAAAAGATAAAAGCGAATCGGTAGAATTTCGAGAAGCTGTATTAGGTTTAGATGTG
ACACCGCATATCTCAAAAGACAACACGATTTTATTAGATTTATTGATTACACAAAATACATTAGGTGCACCGGTAGTGTA
TGATAAAGGCGAAATTGTTTCGATCGATAAACAGGAAATCAATACTCAAGTCGTCGCTCAAGATGGTGAAACCATCGTTT
TAGGTGGGGTGTTTCATGATACGATGACAAAGGGAGTCAATAAAGTACCACTACTAGGGGATTTGCCTTTGCTTAAACAT
GTGTTTAGCCAGGAAACTGAACGTCATCAAAAGCGAGAATTAGTGATTTTTGTCACGCCTCATATTATCAAACCGACCCA
AAGTTTGCCTGAACAAAAAACAACGGGAGTTAAAAAATCTGCAAAATCGAGGTGA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(119-182)

Bacterial type II and III secretion system protein (Secretin)

(264-424)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comE Haemophilus influenzae Rd KW20

61.573

100

0.617
  comE Haemophilus influenzae 86-028NP

60.899

100

0.61
  comE Glaesserella parasuis strain SC1401

49.409

95.27

0.471
  pilQ Vibrio campbellii strain DS40M4

39.524

94.595

0.374
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

39.806

92.793

0.369
  pilQ Vibrio cholerae strain A1552

39.806

92.793

0.369