Detailed information    

insolico Bioinformatically predicted

Overview

Name   comE   Type   Machinery gene
Locus tag   M9413_RS09220 Genome accession   NZ_CP097794
Coordinates   1966421..1967755 (-) Length   444 a.a.
NCBI ID   WP_250265242.1    Uniprot ID   -
Organism   Pasteurella multocida strain 28606     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1961421..1972755
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M9413_RS09195 (M9413_09195) secM 1962939..1963256 (-) 318 WP_233427170.1 secA translation cis-regulator SecM -
  M9413_RS09200 (M9413_09200) - 1963346..1963651 (+) 306 WP_083003370.1 DUF721 domain-containing protein -
  M9413_RS09205 (M9413_09205) - 1963697..1964602 (-) 906 WP_083003372.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -
  M9413_RS09210 (M9413_09210) aroB 1964606..1965694 (-) 1089 WP_083003375.1 3-dehydroquinate synthase -
  M9413_RS09215 (M9413_09215) aroK 1965711..1966238 (-) 528 WP_005717623.1 shikimate kinase AroK -
  M9413_RS09220 (M9413_09220) comE 1966421..1967755 (-) 1335 WP_250265242.1 type IV pilus secretin PilQ Machinery gene
  M9413_RS09225 (M9413_09225) - 1967765..1968127 (-) 363 WP_250265243.1 pilus assembly protein PilP -
  M9413_RS09230 (M9413_09230) - 1968130..1968654 (-) 525 WP_096743656.1 ATPase -
  M9413_RS09235 (M9413_09235) - 1968654..1969169 (-) 516 WP_083003385.1 competence protein ComB -
  M9413_RS09240 (M9413_09240) - 1969209..1969982 (-) 774 WP_083003387.1 pilus assembly protein PilM -
  M9413_RS09245 (M9413_09245) - 1970118..1972679 (+) 2562 WP_250265245.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 444 a.a.        Molecular weight: 49261.70 Da        Isoelectric Point: 8.0156

>NTDB_id=692582 M9413_RS09220 WP_250265242.1 1966421..1967755(-) (comE) [Pasteurella multocida strain 28606]
MWRAFRKLSFVYFLCGVAYVGSSQAQDAEHFYLRLKQAPLVEMLQYLALRQHQDLLIDDHLEGTLSLQMKKTTFEKCLQS
IAKMKQLEFHQEGKSYYLTSSSGVAANDTHHPTSLMTSSIKLYFAKAADVVKSLTSGQGSLLSVGGSLSFDERTNLLLIQ
DEPQSIQRIKALVAEMDKPIEQIAIEARIVTMTDESLQELGVRWGLFQATEQAHTIAGSLAANGFSNIENQLNVNFTTNS
APVGSIALQLAKINGRLLDLELTALEREKHIEIIASPRLLTTNKKSASIKQGTEIPYVMKRGKDKSESVEFREAVLGLDV
TPHISKDNTILLDLLITQNTLGAPVVYDKGEIVSIDKQEINTQVVAQDGETIVLGGVFHDTMTKGVNKVPLLGDLPLLKH
VFSQKTERHQKRELVIFVTPHIIKPTQSLPEQKTTGVKKSAKSR

Nucleotide


Download         Length: 1335 bp        

>NTDB_id=692582 M9413_RS09220 WP_250265242.1 1966421..1967755(-) (comE) [Pasteurella multocida strain 28606]
ATGTGGCGAGCATTCAGAAAACTATCTTTTGTGTACTTTTTATGTGGGGTTGCTTATGTTGGAAGTAGTCAAGCACAAGA
CGCAGAACATTTTTATTTACGTTTAAAACAAGCACCTTTAGTCGAAATGTTACAGTATTTAGCATTGCGACAACATCAGG
ATTTGTTAATCGATGATCATTTAGAGGGCACATTATCATTACAGATGAAAAAGACAACCTTTGAGAAATGTTTACAGTCG
ATTGCAAAAATGAAACAACTGGAATTCCATCAAGAAGGGAAATCCTATTATTTAACTTCCTCTTCAGGTGTTGCAGCAAA
CGATACTCATCATCCTACGTCATTGATGACATCTTCAATAAAATTGTATTTTGCCAAAGCCGCAGATGTAGTGAAATCTT
TAACTTCAGGGCAGGGAAGTTTACTTTCTGTCGGGGGGAGTTTGAGTTTTGATGAGCGGACTAATTTACTGCTGATTCAG
GATGAACCGCAATCAATACAGCGTATTAAAGCATTAGTAGCAGAAATGGATAAACCCATTGAACAAATTGCGATCGAAGC
CAGGATTGTGACGATGACAGACGAAAGTTTGCAGGAACTTGGTGTAAGATGGGGGCTATTTCAAGCAACAGAACAGGCAC
ATACTATTGCGGGGAGTTTAGCCGCGAACGGCTTTTCGAATATAGAAAACCAATTAAATGTGAATTTTACGACCAATAGT
GCACCTGTTGGTTCCATCGCCTTACAGTTGGCGAAAATAAATGGTCGATTATTAGACTTGGAATTAACTGCCTTAGAGCG
AGAAAAGCATATTGAGATTATTGCGAGTCCTCGTTTATTAACAACGAATAAAAAAAGTGCCAGTATCAAACAAGGGACGG
AAATTCCTTATGTGATGAAACGGGGAAAAGATAAAAGCGAATCGGTAGAATTTCGAGAAGCTGTATTAGGTTTAGATGTG
ACACCGCATATCTCAAAAGACAACACGATTTTATTAGATTTATTGATTACACAAAATACATTAGGTGCACCGGTAGTGTA
TGATAAAGGCGAAATTGTTTCGATCGATAAACAGGAAATCAATACTCAAGTCGTCGCTCAAGATGGTGAAACCATCGTTT
TAGGTGGGGTGTTTCATGATACGATGACAAAGGGAGTCAATAAAGTACCACTACTAGGGGATTTGCCTTTGCTTAAACAT
GTGTTTAGCCAGAAAACTGAACGTCATCAAAAGCGGGAATTAGTGATTTTTGTCACGCCTCATATTATCAAACCGACCCA
AAGTTTGCCTGAACAAAAAACAACGGGAGTTAAAAAATCTGCAAAATCGAGGTGA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(119-182)

Bacterial type II and III secretion system protein (Secretin)

(264-424)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comE Haemophilus influenzae Rd KW20

60.674

100

0.608
  comE Haemophilus influenzae 86-028NP

60

100

0.601
  comE Glaesserella parasuis strain SC1401

48.936

95.27

0.466
  pilQ Vibrio campbellii strain DS40M4

39.762

94.595

0.376
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

40.049

92.793

0.372
  pilQ Vibrio cholerae strain A1552

40.049

92.793

0.372