Detailed information    

insolico Bioinformatically predicted

Overview

Name   comE   Type   Machinery gene
Locus tag   M9415_RS10045 Genome accession   NZ_CP097790
Coordinates   2143487..2144821 (-) Length   444 a.a.
NCBI ID   WP_046333760.1    Uniprot ID   -
Organism   Pasteurella multocida strain 31971     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2138487..2149821
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M9415_RS10020 (M9415_10020) secM 2140007..2140321 (-) 315 WP_010907080.1 secA translation cis-regulator SecM -
  M9415_RS10025 (M9415_10025) - 2140411..2140716 (+) 306 WP_005723691.1 DUF721 domain-containing protein -
  M9415_RS10030 (M9415_10030) - 2140763..2141668 (-) 906 WP_005723696.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -
  M9415_RS10035 (M9415_10035) aroB 2141672..2142760 (-) 1089 WP_046333761.1 3-dehydroquinate synthase -
  M9415_RS10040 (M9415_10040) aroK 2142777..2143304 (-) 528 WP_005717623.1 shikimate kinase AroK -
  M9415_RS10045 (M9415_10045) comE 2143487..2144821 (-) 1335 WP_046333760.1 type IV pilus secretin PilQ Machinery gene
  M9415_RS10050 (M9415_10050) - 2144831..2145193 (-) 363 WP_005748555.1 pilus assembly protein PilP -
  M9415_RS10055 (M9415_10055) - 2145196..2145720 (-) 525 WP_046333759.1 hypothetical protein -
  M9415_RS10060 (M9415_10060) - 2145720..2146235 (-) 516 WP_046333758.1 competence protein ComB -
  M9415_RS10065 (M9415_10065) - 2146275..2147048 (-) 774 WP_046333757.1 pilus assembly protein PilM -
  M9415_RS10070 (M9415_10070) - 2147184..2149745 (+) 2562 WP_005757394.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 444 a.a.        Molecular weight: 49312.76 Da        Isoelectric Point: 8.0163

>NTDB_id=692497 M9415_RS10045 WP_046333760.1 2143487..2144821(-) (comE) [Pasteurella multocida strain 31971]
MWRAFRKISFVYFLCGVAYVGSSQAQDAEHFYLRLKQAPLVEMLQYLALQQHQDLLIDDHLEGTLSLQMKKTTFEKCLQS
IARMKQLELHQEGKSYYLTSPSGVAANDTHHPTSLMTSSIKLYFAKAAEVMKSLTSGQGSLLSVGGSLSFDERTNLLLIQ
DEPQSIQRIKALVAEMDKPIEQIAIEARIVTMTDESLQELGVRWGLFQATEQAHTIAGSLAANGFSNIENQLNVNFSTNS
APVGSIALQLAKINGRLLDLELTALEREKHIEIIASPRLLTTNKKSASIKQGTEIPYVMKRGKDKSESVEFREAVLGLDV
TPHISKDNTILLDLLITQNTLGAPVVYDKGEIVSIDKQEINTQVIAQDGETIVLGGVFHDTMTKGVNKVPLLGDLPLLKH
VFSQKTERHQKRELVIFVTPHIIKPSQGSPEQKTTRVKKSAKSR

Nucleotide


Download         Length: 1335 bp        

>NTDB_id=692497 M9415_RS10045 WP_046333760.1 2143487..2144821(-) (comE) [Pasteurella multocida strain 31971]
ATGTGGCGAGCATTCAGAAAAATATCTTTTGTGTACTTTTTATGTGGGGTTGCTTATGTTGGAAGTAGTCAAGCACAAGA
CGCAGAACATTTTTATTTACGTTTAAAACAAGCGCCTTTAGTCGAAATGTTACAGTATTTAGCATTACAACAACATCAGG
ATTTGTTAATCGATGATCATTTAGAGGGCACATTATCATTACAGATGAAAAAGACAACCTTTGAGAAATGTTTACAGTCG
ATTGCAAGAATGAAACAACTTGAGTTACATCAAGAAGGAAAATCCTATTATTTAACTTCCCCTTCAGGTGTTGCAGCAAA
CGATACTCATCATCCTACGTCATTGATGACATCTTCAATAAAATTGTATTTTGCCAAAGCCGCAGAGGTGATGAAATCTT
TAACTTCAGGGCAGGGAAGTTTACTTTCTGTCGGGGGGAGTTTGAGTTTTGATGAGCGGACTAATTTACTGCTGATTCAG
GATGAACCGCAATCAATACAGCGTATTAAAGCATTAGTAGCAGAAATGGATAAACCCATTGAACAAATTGCGATCGAAGC
CAGGATTGTGACGATGACAGACGAAAGTTTGCAGGAACTTGGTGTAAGATGGGGGCTATTTCAAGCAACAGAACAGGCAC
ATACTATTGCAGGAAGTTTAGCCGCGAACGGTTTTTCGAATATAGAAAACCAATTAAATGTGAATTTCTCGACCAATAGT
GCACCTGTTGGTTCCATCGCCTTACAGTTGGCGAAAATAAATGGTCGATTATTAGACTTGGAATTAACTGCCTTGGAGCG
AGAAAAGCATATTGAGATTATTGCGAGTCCTCGTTTATTAACAACGAATAAAAAAAGTGCCAGTATCAAACAAGGGACAG
AAATTCCTTATGTGATGAAACGGGGAAAAGATAAAAGCGAATCGGTGGAATTTCGAGAAGCTGTATTGGGTTTAGATGTG
ACACCGCATATCTCAAAAGACAACACGATTTTATTAGATTTATTGATTACACAAAATACATTAGGTGCACCGGTAGTGTA
TGATAAAGGCGAAATTGTTTCGATCGATAAACAGGAAATCAATACTCAAGTCATCGCTCAAGATGGTGAAACCATCGTTT
TAGGTGGGGTGTTTCATGATACGATGACAAAGGGAGTCAATAAAGTACCACTACTAGGGGATTTGCCTTTGCTTAAACAT
GTGTTTAGCCAGAAAACTGAACGTCATCAAAAGCGGGAATTAGTGATTTTTGTCACGCCTCATATTATCAAACCTAGCCA
AGGTTCGCCTGAACAAAAAACAACAAGAGTTAAAAAATCTGCAAAATCGAGGTGA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(264-424)

Bacterial type II/III secretion system short domain (Secretin_N)

(119-182)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comE Haemophilus influenzae Rd KW20

62.037

97.297

0.604
  comE Haemophilus influenzae 86-028NP

61.343

97.297

0.597
  comE Glaesserella parasuis strain SC1401

48.73

97.523

0.475
  pilQ Vibrio campbellii strain DS40M4

39.524

94.595

0.374
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

39.806

92.793

0.369
  pilQ Vibrio cholerae strain A1552

39.806

92.793

0.369