Detailed information    

insolico Bioinformatically predicted

Overview

Name   comE   Type   Machinery gene
Locus tag   M9421_RS03995 Genome accession   NZ_CP097788
Coordinates   874214..875548 (-) Length   444 a.a.
NCBI ID   WP_250262988.1    Uniprot ID   -
Organism   Pasteurella multocida strain 21317     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 869214..880548
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M9421_RS03970 (M9421_03970) secM 870734..871048 (-) 315 WP_010907080.1 secA translation cis-regulator SecM -
  M9421_RS03975 (M9421_03975) - 871138..871443 (+) 306 WP_083003370.1 DUF721 domain-containing protein -
  M9421_RS03980 (M9421_03980) - 871490..872395 (-) 906 WP_250262986.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -
  M9421_RS03985 (M9421_03985) aroB 872399..873487 (-) 1089 WP_250262987.1 3-dehydroquinate synthase -
  M9421_RS03990 (M9421_03990) aroK 873504..874031 (-) 528 WP_005717623.1 shikimate kinase AroK -
  M9421_RS03995 (M9421_03995) comE 874214..875548 (-) 1335 WP_250262988.1 type IV pilus secretin PilQ Machinery gene
  M9421_RS04000 (M9421_04000) - 875558..875920 (-) 363 WP_115300060.1 pilus assembly protein PilP -
  M9421_RS04005 (M9421_04005) - 875923..876447 (-) 525 WP_315582728.1 ATPase -
  M9421_RS04010 (M9421_04010) - 876447..876962 (-) 516 WP_104884245.1 competence protein ComB -
  M9421_RS04015 (M9421_04015) - 877002..877775 (-) 774 WP_115300057.1 pilus assembly protein PilM -
  M9421_RS04020 (M9421_04020) - 877911..880472 (+) 2562 WP_250262990.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 444 a.a.        Molecular weight: 49247.59 Da        Isoelectric Point: 7.3924

>NTDB_id=692436 M9421_RS03995 WP_250262988.1 874214..875548(-) (comE) [Pasteurella multocida strain 21317]
MWRAFRKLSFVYFLCGVAYVGSSQAQDAEHFYLRLKQAPLVEMLQYLALQQHQDLLIDDHLEGTLSLQMKKTTFEKCLQS
IAKMKQLEFHQEGKSYYLTSPSGVAANDTHHPTSLMTSSIKLHFAKAAEVVKSLTSGQGSLLSVGGSLSFDERTNLLLIQ
DEPQSIQRIKALVAEMDKPIEQIAIEARIVTMTDESLQELGVRWGLFQATEQAHTIAGSLAANGFSNIENQLNVNFSTNS
APVGSIALQLAKINGRLLDLELTALEREKHIEIIASPRLLTTNKKSASIKQGTEIPYVMKRGKDKSESVEFREAVLGLDV
TPHISKDNTILLDLLITQNTLGAPVVYDKGEIVSIDKQEINTQVVAQDGETIVLGGVFHDTMTKGVNKVPLLGDLPLLKH
VFSQETERHQKRELVIFVTPHIIKPSQGSPEQKTTRVKKSAKSR

Nucleotide


Download         Length: 1335 bp        

>NTDB_id=692436 M9421_RS03995 WP_250262988.1 874214..875548(-) (comE) [Pasteurella multocida strain 21317]
ATGTGGCGAGCATTCAGAAAACTATCTTTTGTGTACTTTTTATGTGGGGTTGCTTATGTTGGAAGTAGTCAAGCACAAGA
CGCAGAACATTTTTATTTACGTTTAAAACAAGCACCTTTAGTCGAAATGTTACAGTATTTAGCATTGCAACAACATCAGG
ATTTGTTAATCGATGATCATTTAGAGGGCACATTATCATTACAGATGAAAAAGACAACCTTTGAGAAATGTTTACAGTCG
ATTGCAAAAATGAAACAACTTGAATTCCATCAAGAAGGAAAATCCTATTATTTAACTTCCCCTTCAGGTGTTGCGGCAAA
CGATACTCATCATCCTACGTCATTGATGACATCTTCAATAAAATTGCATTTTGCCAAAGCCGCAGAGGTGGTGAAATCTT
TAACTTCAGGGCAGGGAAGTTTACTTTCTGTCGGGGGGAGTTTGAGTTTTGATGAGCGGACTAATTTACTGCTGATTCAG
GATGAACCGCAATCAATACAGCGTATTAAAGCATTAGTAGCAGAAATGGATAAACCCATTGAACAAATTGCGATCGAAGC
CAGGATTGTGACGATGACAGACGAAAGTTTGCAGGAACTTGGTGTAAGATGGGGGCTATTTCAAGCAACAGAACAGGCAC
ATACTATTGCGGGGAGTTTAGCCGCGAACGGCTTTTCGAATATAGAAAACCAATTAAATGTGAATTTCTCGACCAATAGT
GCACCTGTTGGTTCCATCGCCTTACAGTTGGCGAAAATAAATGGTCGATTATTAGACTTGGAATTAACTGCCTTGGAGCG
AGAAAAGCATATTGAGATTATTGCGAGTCCTCGTTTATTAACAACGAATAAAAAAAGTGCCAGTATCAAACAAGGGACGG
AAATTCCTTATGTGATGAAACGGGGAAAAGATAAAAGCGAATCGGTGGAATTTCGAGAAGCTGTATTAGGTTTAGATGTG
ACACCGCATATTTCAAAAGACAACACGATTTTATTAGATTTATTGATTACACAAAATACATTAGGTGCACCGGTAGTGTA
TGATAAAGGCGAAATTGTTTCGATCGATAAACAGGAAATCAATACTCAAGTCGTCGCTCAAGATGGTGAAACCATCGTTT
TAGGTGGGGTGTTTCATGATACGATGACAAAGGGAGTCAATAAGGTACCACTACTAGGGGATTTGCCTTTGCTTAAACAT
GTGTTTAGCCAGGAAACTGAACGTCATCAAAAGCGGGAATTAGTGATTTTTGTCACGCCTCATATTATCAAACCTAGCCA
AGGTTCGCCTGAACAAAAAACAACAAGAGTTAAAAAATCTGCAAAATCAAGGTGA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(264-424)

Bacterial type II/III secretion system short domain (Secretin_N)

(119-182)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comE Haemophilus influenzae Rd KW20

62.269

97.297

0.606
  comE Haemophilus influenzae 86-028NP

61.574

97.297

0.599
  comE Glaesserella parasuis strain SC1401

48.961

97.523

0.477
  pilQ Vibrio campbellii strain DS40M4

39.524

94.595

0.374
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

39.806

92.793

0.369
  pilQ Vibrio cholerae strain A1552

39.806

92.793

0.369