Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   OZ175_RS20845 Genome accession   NZ_CP113974
Coordinates   4448758..4449381 (-) Length   207 a.a.
NCBI ID   WP_003103268.1    Uniprot ID   G3XD51
Organism   Pseudomonas aeruginosa strain M6A146     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4443758..4454381
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OZ175_RS20825 (OZ175_20825) aroB 4444354..4445460 (-) 1107 WP_003107839.1 3-dehydroquinate synthase -
  OZ175_RS20830 (OZ175_20830) aroK 4445509..4446027 (-) 519 WP_003095833.1 shikimate kinase AroK -
  OZ175_RS20835 (OZ175_20835) pilQ 4446039..4448183 (-) 2145 WP_023087871.1 type 4a pilus secretin PilQ Machinery gene
  OZ175_RS20840 (OZ175_20840) pilP 4448279..4448761 (-) 483 WP_268858299.1 type 4a pilus biogenesis lipoprotein PilP -
  OZ175_RS20845 (OZ175_20845) pilO 4448758..4449381 (-) 624 WP_003103268.1 type 4a pilus biogenesis protein PilO Machinery gene
  OZ175_RS20850 (OZ175_20850) pilN 4449378..4449974 (-) 597 WP_003095839.1 type 4a pilus biogenesis protein PilN Machinery gene
  OZ175_RS20855 (OZ175_20855) pilM 4449974..4451038 (-) 1065 WP_003110888.1 type IV pilus assembly protein PilM Machinery gene
  OZ175_RS20860 (OZ175_20860) - 4451223..4453694 (+) 2472 WP_268858300.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 207 a.a.        Molecular weight: 22819.26 Da        Isoelectric Point: 4.7787

>NTDB_id=692002 OZ175_RS20845 WP_003103268.1 4448758..4449381(-) (pilO) [Pseudomonas aeruginosa strain M6A146]
MSLASSLESLRKIDINDLDLNNIGSWPAAVKVIVCVLLTAAVLALGYNFHLSDMQAQLEQQAAEEETLKQQFSTKAFQAA
NLEAYKAQMKEMEESFGALLRQLPSDTEVPGLLEDITRTGLGSGLEFEEIKLLPEVAQQFYIELPIQISVVGGYHDLATF
VSGVSSLPRIVTLHDFEIKPVAPGSTSKLRMSILAKTYRYNDKGLKK

Nucleotide


Download         Length: 624 bp        

>NTDB_id=692002 OZ175_RS20845 WP_003103268.1 4448758..4449381(-) (pilO) [Pseudomonas aeruginosa strain M6A146]
ATGAGTCTGGCCAGTTCCCTGGAAAGTCTGCGCAAGATCGATATCAACGATCTCGACCTGAACAACATCGGTTCCTGGCC
GGCGGCGGTCAAGGTCATCGTCTGCGTGCTGCTGACCGCGGCGGTCCTGGCGCTGGGCTACAACTTCCATCTGAGCGACA
TGCAGGCTCAGCTCGAACAGCAGGCCGCGGAAGAGGAGACGCTCAAGCAGCAGTTCTCCACCAAGGCCTTCCAGGCCGCG
AACCTGGAAGCCTACAAGGCACAGATGAAGGAGATGGAAGAGTCCTTCGGTGCCTTGCTGCGGCAGTTGCCCAGCGACAC
CGAGGTACCCGGGCTGCTCGAGGACATCACTCGTACCGGCCTGGGCAGCGGCCTGGAGTTCGAGGAAATCAAGCTGCTTC
CCGAGGTTGCCCAGCAGTTCTACATCGAGCTGCCGATCCAGATCAGCGTGGTCGGCGGCTACCACGACCTGGCGACCTTC
GTCAGCGGCGTGTCCAGCCTGCCGCGGATCGTCACCCTGCATGACTTCGAGATCAAGCCGGTCGCGCCCGGCAGCACGTC
CAAGCTGCGCATGAGCATCCTGGCCAAGACCTATCGCTACAATGACAAGGGGCTGAAGAAATGA

Domains

Predicted by InterProScan.

Pilus assembly protein, PilO (PilO)

(58-202)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB G3XD51

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Legionella pneumophila strain ERS1305867

46.114

93.237

0.43
  pilO Acinetobacter baumannii D1279779

43.979

92.271

0.406
  comO/pilO Acinetobacter baylyi ADP1

43.455

92.271

0.401