Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   EP178_RS00660 Genome accession   NZ_AP018377
Coordinates   103943..106138 (-) Length   731 a.a.
NCBI ID   WP_003687350.1    Uniprot ID   A0AAQ1ILV8
Organism   Neisseria gonorrhoeae strain FC428     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 98943..111138
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EP178_RS00625 (NgFC428_00087) - 99423..100265 (-) 843 WP_003687345.1 class II glutamine amidotransferase -
  EP178_RS00630 (NgFC428_00088) aroB 101004..102083 (-) 1080 WP_003696677.1 3-dehydroquinate synthase -
  EP178_RS00640 (NgFC428_00089) - 102163..102675 (-) 513 WP_003687349.1 shikimate kinase -
  EP178_RS00660 (NgFC428_00090) pilQ 103943..106138 (-) 2196 WP_003687350.1 type IV pilus secretin PilQ Machinery gene
  EP178_RS00665 (NgFC428_00091) pilP 106157..106702 (-) 546 WP_003692554.1 pilus assembly protein PilP Machinery gene
  EP178_RS00670 (NgFC428_00092) - 106720..107367 (-) 648 WP_003690570.1 type 4a pilus biogenesis protein PilO -
  EP178_RS00675 (NgFC428_00093) pilN 107368..107967 (-) 600 WP_047926976.1 PilN domain-containing protein Machinery gene
  EP178_RS00680 (NgFC428_00094) pilM 107970..109085 (-) 1116 WP_003696683.1 type IV pilus biogenesis protein PilM -

Sequence

Protein


Download         Length: 731 a.a.        Molecular weight: 78773.85 Da        Isoelectric Point: 10.0442

>NTDB_id=69156 EP178_RS00660 WP_003687350.1 103943..106138(-) (pilQ) [Neisseria gonorrhoeae strain FC428]
MNTKLTKIISGLFVATAAFQTASAGNITDIKVSSLPNKQKIVKVSFDKEIVNPTGFVTSSPARIALDFEQTGISMDQQVL
EYADPLLSKISAAQNSSRARLVLNLNKPGQYNTEVRGNKVWIFINESDDTVSAPARPAVKAAPAAPAKQQAAAPFTESVV
SVSAPFSPAKQQAAASAKQQAATPAKQTNIDFRKDGKNAGIIELAALGFAGQPDISQQHDHIIVTLKNHTLPTALQRSLD
VADFKTPVQKVTLKRLNNDTQLIITTTGNWELVNKSAAPGYFTFQVLPKKQNLESGGVNNAPKTFTGRKISLDFQDVEIR
TILQILAKESGMNIVASDSVNGKMTLSLKDVPWDQALDLVMQARNLDMRQQGNIVNIAPRDELLAKDKAFLQAEKDIADL
GALYSQNFQLKYKNVEEFRSILRLDNADTTGNRNTLVSGRGSVLIDPATNTLIVTDTRSVIEKFRKLIDELDVPAQQVMI
EARIVEAADGFSRDLGVKFGATGRKKLKNETSAFGWGVNSGFGGGDKWEAQTKINLPVAAAANSISLVRAISSGALNLEL
SASESLSKTKTLANPRVLTQNRKEAKIESGYEIPFTVTTASGGGNSTNTELKKAVLGLTVTPNITPDGQIIMTVKINKDS
PAQCASGNNTILCISTKSLNTQAMVENGGTLIVGGIYEENNGNTLTKVPLLGDIPVIGNLFKTRGKKTDRRELLIFITPR
IIDTAGNSLRY

Nucleotide


Download         Length: 2196 bp        

>NTDB_id=69156 EP178_RS00660 WP_003687350.1 103943..106138(-) (pilQ) [Neisseria gonorrhoeae strain FC428]
ATGAATACCAAACTGACAAAAATCATTTCCGGTCTCTTTGTCGCAACCGCCGCCTTTCAGACGGCATCGGCAGGAAACAT
TACAGACATCAAAGTTTCCTCCCTGCCCAACAAACAGAAAATCGTCAAAGTCAGCTTTGACAAAGAGATTGTCAACCCGA
CCGGCTTCGTAACCTCCTCACCGGCCCGCATCGCCTTGGACTTTGAACAAACCGGCATTTCCATGGATCAACAGGTACTC
GAATATGCCGATCCTCTGTTGAGCAAAATCAGTGCCGCACAAAACAGCAGCCGTGCGCGTCTGGTTCTGAATTTGAACAA
ACCGGGCCAATACAATACCGAAGTACGCGGGAACAAAGTTTGGATATTCATTAACGAATCGGACGATACCGTGTCCGCCC
CCGCCCGCCCAGCCGTAAAAGCCGCGCCTGCCGCACCGGCAAAACAACAGGCTGCCGCACCGTTTACCGAGTCCGTAGTA
TCCGTATCCGCACCGTTCAGCCCGGCAAAACAACAGGCAGCGGCATCGGCAAAACAACAGGCGGCGACACCGGCAAAACA
AACCAATATCGATTTCCGCAAAGACGGCAAAAATGCCGGCATTATCGAATTGGCGGCATTGGGCTTTGCCGGGCAGCCCG
ACATCAGCCAACAGCACGACCACATCATCGTTACGCTGAAAAACCATACCCTGCCGACCGCGCTCCAACGCAGTTTGGAT
GTGGCAGACTTCAAAACACCGGTTCAAAAGGTTACGCTGAAACGCCTCAATAACGACACCCAGCTGATTATCACAACAAC
CGGCAACTGGGAACTCGTCAACAAATCCGCCGCGCCCGGATACTTTACCTTCCAAGTCCTGCCGAAAAAACAAAACCTCG
AGTCAGGCGGCGTGAACAATGCGCCCAAAACCTTCACAGGCCGGAAAATCTCCCTTGACTTCCAAGATGTCGAAATCCGC
ACCATCCTGCAGATTTTGGCAAAAGAATCCGGGATGAACATTGTTGCCAGCGACTCCGTCAACGGCAAAATGACCCTCTC
CCTCAAAGATGTGCCTTGGGATCAGGCTTTGGATTTGGTTATGCAGGCGCGCAACCTCGATATGCGCCAGCAAGGGAACA
TCGTCAACATCGCGCCCCGCGACGAGCTGCTTGCCAAAGACAAAGCCTTCTTACAAGCGGAAAAAGACATTGCCGATCTG
GGCGCGCTGTATTCCCAAAACTTCCAATTGAAATACAAAAATGTGGAAGAATTCCGCAGCATCCTGCGTTTGGACAATGC
CGACACGACCGGAAACCGCAACACGCTTGTCAGCGGCAGGGGCAGCGTGCTGATCGATCCCGCCACCAACACCCTGATTG
TTACCGATACCCGCAGCGTCATCGAAAAATTCCGCAAACTGATTGACGAATTGGACGTACCCGCGCAACAAGTGATGATT
GAGGCGCGTATCGTCGAAGCGGCAGACGGCTTCTCGCGCGATTTGGGCGTTAAGTTCGGCGCGACAGGCAGGAAAAAACT
GAAAAATGAGACGAGCGCATTCGGCTGGGGCGTGAACTCCGGCTTCGGGGGCGGCGATAAATGGGAGGCCCAAACCAAAA
TCAACCTGCCGGTTGCCGCTGCCGCAAACAGCATTTCGCTGGTGCGCGCGATTTCCTCCGGCGCGTTGAATTTGGAATTG
TCCGCATCCGAGTCGCTTTCAAAAACCAAAACGCTTGCCAATCCGCGCGTGCTGACCCAAAACCGCAAAGAGGCCAAAAT
CGAATCCGGTTACGAAATTCCTTTTACCGTAACTACAGCCTCGGGCGGCGGCAACTCTACCAACACGGAACTCAAAAAAG
CCGTCTTGGGGCTGACCGTTACGCCGAACATCACGCCCGACGGACAAATCATCATGACCGTCAAAATCAACAAAGACTCG
CCTGCACAATGTGCTTCAGGCAACAACACAATCCTATGTATTTCGACCAAAAGCCTGAATACGCAGGCTATGGTTGAAAA
CGGCGGCACTTTGATTGTCGGCGGTATTTATGAAGAAAACAACGGCAATACGCTGACCAAAGTCCCCCTGTTGGGCGACA
TCCCCGTTATCGGCAACCTCTTTAAAACACGCGGGAAAAAAACCGACCGCCGCGAACTGCTGATTTTCATTACCCCGAGG
ATTATAGATACGGCCGGCAACAGCCTGCGCTATTGA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(406-477)

Bacterial type II and III secretion system protein (Secretin)

(563-723)

Secretin and TonB N terminus short domain (STN)

(333-378)

AMIN domain (AMIN)

(43-116)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Neisseria gonorrhoeae strain FA1090

98.769

100

0.988
  pilQ Neisseria meningitidis MC58

91.027

100

0.958
  pilQ Neisseria meningitidis M1080

91.721

100

0.955

Multiple sequence alignment