Detailed information    

insolico Bioinformatically predicted

Overview

Name   comE   Type   Machinery gene
Locus tag   M8855_RS10350 Genome accession   NZ_CP097620
Coordinates   2203785..2205119 (+) Length   444 a.a.
NCBI ID   WP_249984261.1    Uniprot ID   -
Organism   Pasteurella multocida strain P2192     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2198785..2210119
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M8855_RS10325 (M8855_10325) - 2198861..2201422 (-) 2562 WP_005754757.1 penicillin-binding protein 1A -
  M8855_RS10330 (M8855_10330) - 2201558..2202331 (+) 774 WP_046333757.1 pilus assembly protein PilM -
  M8855_RS10335 (M8855_10335) - 2202371..2202886 (+) 516 WP_005751904.1 hypothetical protein -
  M8855_RS10340 (M8855_10340) - 2202886..2203410 (+) 525 WP_046333759.1 hypothetical protein -
  M8855_RS10345 (M8855_10345) - 2203413..2203775 (+) 363 WP_005748555.1 pilus assembly protein PilP -
  M8855_RS10350 (M8855_10350) comE 2203785..2205119 (+) 1335 WP_249984261.1 type IV pilus secretin PilQ Machinery gene
  M8855_RS10355 (M8855_10355) aroK 2205302..2205829 (+) 528 WP_005717623.1 shikimate kinase AroK -
  M8855_RS10360 (M8855_10360) aroB 2205846..2206934 (+) 1089 WP_249984260.1 3-dehydroquinate synthase -
  M8855_RS10365 (M8855_10365) - 2206938..2207843 (+) 906 WP_005757382.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -
  M8855_RS10370 (M8855_10370) - 2207890..2208195 (-) 306 WP_005723691.1 DUF721 domain-containing protein -
  M8855_RS10375 (M8855_10375) secM 2208285..2208599 (+) 315 WP_010907080.1 secA translation cis-regulator SecM -

Sequence

Protein


Download         Length: 444 a.a.        Molecular weight: 49244.69 Da        Isoelectric Point: 8.0309

>NTDB_id=691313 M8855_RS10350 WP_249984261.1 2203785..2205119(+) (comE) [Pasteurella multocida strain P2192]
MWRAFRKISFVYFLCGVAYVGSSQAQDAEHFYLRLKQAPLVEMLQYLALQQHQDLLIDDHLEGTLSLQMKKTTFEKCLQS
IAKMKQLELHQEGKSYYLTSPSGVAANDTHHPTSLMTSSIKLHFAKAAEVMKSLTSGQGSLLSVGGSLSFDERTNLLLIQ
DEPQSIQRIKALVAEMDKPIEQIAIEARIVTMTDESLQELGVRWGLFQATEQAHTIAGSLAANGFSNIENQLNVNFSTNS
APVGSIALQLAKINGRLLDLELTALEREKHIEIIASPRLLTTNKKSASIKQGTEIPYVMKRGKDKSESVEFREAVLGLDV
TPHISKDNTILLDLLITQNTLGAPVVYDKGEIVSIDKQEINTQVVAQDGETIVLGGVFHDTMTKGVNKVPLLGDLPLLKH
VFSQKTERHQKRELVIFVTPHIIKPSQGSPEQKTTRVKKSAKSR

Nucleotide


Download         Length: 1335 bp        

>NTDB_id=691313 M8855_RS10350 WP_249984261.1 2203785..2205119(+) (comE) [Pasteurella multocida strain P2192]
ATGTGGCGAGCATTCAGAAAAATATCTTTTGTGTACTTTTTATGTGGGGTTGCTTATGTTGGAAGTAGTCAAGCACAAGA
CGCAGAACATTTTTATTTACGTTTAAAACAAGCGCCTTTAGTCGAAATGTTACAGTATTTAGCATTACAACAACATCAGG
ATTTGTTAATCGATGATCATTTAGAGGGCACATTATCATTACAGATGAAAAAGACAACCTTTGAGAAATGTTTACAGTCG
ATTGCAAAAATGAAACAACTTGAGTTGCATCAAGAAGGAAAATCCTATTATTTAACTTCCCCTTCAGGTGTTGCAGCAAA
CGATACTCATCATCCTACGTCATTGATGACATCTTCAATAAAATTGCATTTTGCCAAAGCCGCAGAGGTGATGAAATCTT
TAACTTCAGGGCAGGGAAGTTTACTTTCTGTCGGGGGGAGTTTGAGTTTTGATGAGCGGACTAATTTACTGCTGATTCAG
GATGAACCGCAATCAATACAGCGTATTAAAGCATTAGTAGCAGAAATGGATAAACCCATTGAACAAATTGCGATCGAAGC
TAGGATTGTGACGATGACAGACGAAAGTTTGCAGGAACTTGGTGTAAGATGGGGGCTATTTCAAGCAACAGAACAGGCAC
ATACTATTGCAGGGAGTTTAGCCGCGAACGGCTTTTCGAATATAGAAAACCAATTAAATGTGAATTTCTCGACCAATAGT
GCACCTGTTGGTTCCATCGCCTTACAGTTGGCGAAAATAAATGGTCGATTATTAGACTTGGAATTAACTGCCTTGGAGCG
AGAAAAGCATATTGAGATTATTGCGAGTCCTCGTTTATTAACAACGAATAAAAAAAGTGCCAGTATCAAACAAGGAACGG
AAATTCCTTATGTGATGAAACGGGGAAAAGATAAAAGCGAATCGGTGGAATTTCGAGAAGCTGTATTAGGTTTAGATGTG
ACACCGCATATCTCGAAAGACAACACGATTTTATTAGATTTATTGATTACACAAAATACATTAGGTGCACCGGTAGTGTA
TGATAAAGGCGAAATTGTTTCGATCGATAAACAAGAAATCAATACTCAAGTCGTCGCTCAAGATGGTGAAACCATCGTTT
TAGGTGGGGTGTTTCATGATACGATGACAAAGGGAGTCAATAAAGTACCACTACTAGGGGATTTGCCTTTGCTTAAACAT
GTGTTTAGCCAGAAAACTGAACGTCATCAAAAGCGGGAATTAGTGATTTTTGTCACGCCTCATATTATCAAACCTAGCCA
AGGTTCGCCTGAACAAAAAACAACAAGAGTTAAAAAATCTGCAAAATCGAGGTGA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(119-182)

Bacterial type II and III secretion system protein (Secretin)

(264-424)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comE Haemophilus influenzae Rd KW20

62.5

97.297

0.608
  comE Haemophilus influenzae 86-028NP

61.806

97.297

0.601
  comE Glaesserella parasuis strain SC1401

49.192

97.523

0.48
  pilQ Vibrio campbellii strain DS40M4

39.524

94.595

0.374
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

39.806

92.793

0.369
  pilQ Vibrio cholerae strain A1552

39.806

92.793

0.369