Detailed information    

insolico Bioinformatically predicted

Overview

Name   comE   Type   Machinery gene
Locus tag   M8854_RS10740 Genome accession   NZ_CP097618
Coordinates   2277672..2279006 (+) Length   444 a.a.
NCBI ID   WP_064965220.1    Uniprot ID   -
Organism   Pasteurella multocida strain P2095     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2272672..2284006
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M8854_RS10715 (M8854_10715) - 2272748..2275309 (-) 2562 WP_064965223.1 penicillin-binding protein 1A -
  M8854_RS10720 (M8854_10720) - 2275445..2276218 (+) 774 WP_064965222.1 pilus assembly protein PilM -
  M8854_RS10725 (M8854_10725) - 2276258..2276773 (+) 516 WP_005751904.1 hypothetical protein -
  M8854_RS10730 (M8854_10730) - 2276773..2277297 (+) 525 WP_046333759.1 hypothetical protein -
  M8854_RS10735 (M8854_10735) - 2277300..2277662 (+) 363 WP_064965221.1 pilus assembly protein PilP -
  M8854_RS10740 (M8854_10740) comE 2277672..2279006 (+) 1335 WP_064965220.1 type IV pilus secretin PilQ Machinery gene
  M8854_RS10745 (M8854_10745) aroK 2279189..2279716 (+) 528 WP_005717623.1 shikimate kinase AroK -
  M8854_RS10750 (M8854_10750) aroB 2279733..2280821 (+) 1089 WP_064965219.1 3-dehydroquinate synthase -
  M8854_RS10755 (M8854_10755) - 2280825..2281730 (+) 906 WP_005723696.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -
  M8854_RS10760 (M8854_10760) - 2281777..2282082 (-) 306 WP_005723691.1 DUF721 domain-containing protein -
  M8854_RS10765 (M8854_10765) secM 2282172..2282486 (+) 315 WP_010907080.1 secA translation cis-regulator SecM -

Sequence

Protein


Download         Length: 444 a.a.        Molecular weight: 49286.73 Da        Isoelectric Point: 8.0315

>NTDB_id=691292 M8854_RS10740 WP_064965220.1 2277672..2279006(+) (comE) [Pasteurella multocida strain P2095]
MWRAFRKISFVYFLCGVAYVGSSQAQDAEHFYLRLKQAPLVEMLQYLALQQHQDLLIDDHLEGTLSLQMKKTTFEKCLQS
IARMKQLELHQEGKSYYLTSPSGVAANDTHHPTSLMTSSIKLHFAKAAEVMKSLTSGQGSLLSVGGSLSFDERTNLLLIQ
DEPQSIQRIKALVAEMDKPIEQIAIEARIVTMTDESLQELGVRWGLFQATEQAHTIAGSLAANGFSNIENQLNVNFSTNS
APVGSIALQLAKINGRLLDLELTALEREKHIEIIASPRLLTTNKKSASIKQGTEIPYVMKRGKDKSESVEFREAVLGLDV
TPHISKDNTILLDLLITQNTLGAPVVYDKGEIVSIDKQEINTQVIAQDGETIVLGGVFHDTMTKGVNKVPLLGDLPLLKH
VFSQKTERHQKRELVIFVTPHIIKPSQGSPEQKTTRVKKSAKSR

Nucleotide


Download         Length: 1335 bp        

>NTDB_id=691292 M8854_RS10740 WP_064965220.1 2277672..2279006(+) (comE) [Pasteurella multocida strain P2095]
ATGTGGCGAGCATTCAGAAAAATATCTTTTGTGTACTTTTTATGTGGGGTTGCTTATGTTGGAAGTAGTCAAGCACAAGA
CGCAGAACATTTTTATTTACGTTTAAAACAAGCGCCTTTAGTTGAAATGTTACAGTATTTAGCATTACAACAACATCAGG
ATTTGTTAATCGATGATCATTTAGAGGGCACATTATCATTACAGATGAAAAAGACAACCTTTGAGAAATGTTTACAGTCG
ATTGCAAGAATGAAACAACTTGAGTTACATCAAGAAGGAAAATCCTATTATTTAACTTCCCCTTCAGGTGTTGCAGCAAA
CGATACTCATCATCCTACGTCATTGATGACATCTTCAATAAAATTGCATTTTGCCAAAGCCGCAGAGGTGATGAAATCTT
TAACTTCAGGGCAGGGAAGTTTACTTTCTGTCGGGGGGAGTTTGAGTTTTGATGAGCGGACTAATTTACTGCTGATTCAG
GATGAACCGCAATCAATACAGCGTATTAAAGCATTAGTAGCAGAAATGGATAAACCCATTGAACAAATTGCGATCGAAGC
CAGGATTGTGACGATGACAGACGAAAGTTTGCAGGAACTTGGTGTAAGATGGGGGCTATTTCAAGCAACAGAACAGGCAC
ATACTATTGCAGGAAGTTTAGCCGCGAACGGTTTTTCGAATATAGAAAACCAATTAAATGTGAATTTCTCGACCAATAGT
GCACCTGTTGGTTCCATCGCCTTACAGTTGGCGAAAATAAATGGTCGATTATTAGACTTGGAATTAACTGCCTTGGAGCG
AGAAAAGCATATTGAGATTATTGCGAGTCCTCGTTTATTAACAACGAATAAAAAAAGTGCCAGTATCAAACAAGGGACAG
AAATTCCTTATGTGATGAAACGGGGAAAAGATAAAAGCGAATCGGTGGAATTTCGAGAAGCTGTATTGGGTTTAGATGTG
ACACCGCATATCTCAAAAGACAACACGATTTTATTAGATTTATTGATTACACAAAATACATTAGGTGCACCGGTAGTGTA
TGATAAAGGCGAAATTGTTTCGATCGATAAACAGGAAATCAATACTCAAGTCATCGCTCAAGATGGTGAAACCATCGTTT
TAGGTGGGGTGTTTCATGATACGATGACAAAGGGAGTCAATAAAGTACCACTACTAGGGGATTTGCCTTTGCTTAAACAT
GTGTTTAGCCAGAAAACTGAACGTCATCAAAAGCGGGAATTAGTGATTTTTGTCACGCCTCATATTATCAAACCTAGCCA
AGGTTCGCCTGAACAAAAAACAACAAGAGTTAAAAAATCTGCAAAATCGAGGTGA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(264-424)

Bacterial type II/III secretion system short domain (Secretin_N)

(119-182)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comE Haemophilus influenzae Rd KW20

62.269

97.297

0.606
  comE Haemophilus influenzae 86-028NP

61.574

97.297

0.599
  comE Glaesserella parasuis strain SC1401

48.961

97.523

0.477
  pilQ Vibrio campbellii strain DS40M4

39.524

94.595

0.374
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

39.806

92.793

0.369
  pilQ Vibrio cholerae strain A1552

39.806

92.793

0.369