Detailed information    

insolico Bioinformatically predicted

Overview

Name   comE   Type   Machinery gene
Locus tag   M8993_RS10280 Genome accession   NZ_CP097605
Coordinates   2219498..2220832 (+) Length   444 a.a.
NCBI ID   WP_169029542.1    Uniprot ID   -
Organism   Pasteurella multocida strain 10185     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2214498..2225832
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M8993_RS10255 (M8993_10255) - 2214574..2217135 (-) 2562 WP_059246302.1 penicillin-binding protein 1A -
  M8993_RS10260 (M8993_10260) - 2217271..2218044 (+) 774 WP_046333757.1 pilus assembly protein PilM -
  M8993_RS10265 (M8993_10265) - 2218084..2218599 (+) 516 WP_046333758.1 competence protein ComB -
  M8993_RS10270 (M8993_10270) - 2218599..2219123 (+) 525 WP_046333759.1 hypothetical protein -
  M8993_RS10275 (M8993_10275) - 2219126..2219488 (+) 363 WP_005748555.1 pilus assembly protein PilP -
  M8993_RS10280 (M8993_10280) comE 2219498..2220832 (+) 1335 WP_169029542.1 type IV pilus secretin PilQ Machinery gene
  M8993_RS10285 (M8993_10285) aroK 2221015..2221542 (+) 528 WP_005717623.1 shikimate kinase AroK -
  M8993_RS10290 (M8993_10290) aroB 2221559..2222647 (+) 1089 WP_005754750.1 3-dehydroquinate synthase -
  M8993_RS10295 (M8993_10295) - 2222651..2223556 (+) 906 WP_005757382.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -
  M8993_RS10300 (M8993_10300) - 2223603..2223908 (-) 306 WP_005723691.1 DUF721 domain-containing protein -
  M8993_RS10305 (M8993_10305) secM 2223998..2224312 (+) 315 WP_010907080.1 secA translation cis-regulator SecM -

Sequence

Protein


Download         Length: 444 a.a.        Molecular weight: 49284.71 Da        Isoelectric Point: 8.0163

>NTDB_id=691096 M8993_RS10280 WP_169029542.1 2219498..2220832(+) (comE) [Pasteurella multocida strain 10185]
MWRAFRKISFVYFLCGVAYVGSSQAQDAEHFYLRLKQAPLVEMLQYLALQQHQDLLIDDHLEGTLSLQMKKTTFEKCLQS
IARMKQLELHQEGKSYYLTSPSGVAANDTHHPTSLMTSSIKLHFAKAAEVMKSLTSGQGSLLSVGGSLSFDERTNLLLIQ
DEPQSIQRIKALVAEMDKPIEQIAIEARIVTMTDESLQELGVRWGLFQATEQAHTIAGSLAANGFSNIENQLNVNFSTNS
APVGSIALQLAKINGRLLDLELTALEREKHIEIIASPRLLTTNKKSASIKQGTEIPYVMKRGKDKSESVEFREAVLGLDV
TPHISKDNSILLDLLITQNTLGAPVVYDKGEIVSIDKQEINTQVVAQDGETIVLGGVFHDTMTKGVNKVPLLGDLPLLKY
VFSQKTERHQKRELVIFVTPHIIKPSQGSPEQKTTRVKKSAKSR

Nucleotide


Download         Length: 1335 bp        

>NTDB_id=691096 M8993_RS10280 WP_169029542.1 2219498..2220832(+) (comE) [Pasteurella multocida strain 10185]
ATGTGGCGAGCATTCAGAAAAATATCTTTTGTGTACTTTTTATGTGGGGTTGCTTATGTTGGAAGTAGTCAAGCACAAGA
CGCAGAACATTTTTATTTACGTTTAAAACAAGCGCCTTTAGTCGAAATGTTACAGTATTTAGCATTACAACAACATCAGG
ATTTGTTAATCGATGATCATTTAGAGGGCACATTATCATTACAGATGAAAAAGACAACCTTTGAGAAATGTTTACAGTCG
ATTGCAAGAATGAAACAACTTGAGTTACATCAAGAAGGAAAATCCTATTATTTAACTTCCCCTTCAGGTGTTGCAGCAAA
CGATACTCATCATCCTACGTCATTGATGACATCTTCAATAAAATTGCATTTTGCCAAAGCCGCAGAGGTGATGAAATCTT
TAACTTCAGGGCAGGGAAGTTTACTTTCTGTCGGGGGGAGTTTGAGTTTTGATGAGCGGACTAATTTACTGCTGATTCAG
GATGAACCGCAATCAATACAGCGTATTAAAGCATTAGTAGCAGAAATGGATAAACCCATTGAACAAATTGCGATCGAAGC
TAGGATTGTGACGATGACAGACGAAAGTTTGCAGGAACTTGGTGTAAGATGGGGGCTATTTCAAGCAACAGAACAGGCAC
ATACTATTGCAGGGAGTTTAGCCGCGAACGGCTTTTCGAATATAGAAAACCAATTAAATGTGAATTTCTCGACCAATAGT
GCACCTGTTGGTTCCATCGCCTTACAGTTGGCGAAAATAAATGGTCGATTATTAGACTTGGAATTAACTGCCTTGGAGCG
AGAAAAGCATATTGAGATTATTGCGAGTCCTCGTTTATTAACAACGAATAAAAAAAGTGCCAGTATCAAACAAGGGACGG
AAATTCCTTATGTGATGAAACGGGGAAAAGATAAAAGCGAATCGGTGGAATTTCGAGAAGCTGTATTGGGTTTAGATGTG
ACACCGCATATCTCAAAAGACAACTCGATTTTATTAGATTTATTGATTACACAAAATACATTAGGTGCACCGGTAGTGTA
TGATAAAGGCGAAATTGTTTCGATCGATAAACAGGAAATCAATACTCAAGTCGTCGCTCAAGATGGTGAAACCATCGTTT
TAGGTGGGGTGTTTCATGATACGATGACAAAGGGAGTCAATAAAGTACCACTACTAGGGGATTTGCCTTTGCTTAAATAT
GTGTTTAGCCAGAAAACTGAACGTCATCAAAAGCGGGAATTAGTGATTTTTGTCACTCCTCATATTATCAAACCTAGCCA
AGGTTCGCCTGAACAAAAAACAACAAGAGTTAAAAAATCTGCAAAATCAAGGTGA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(119-182)

Bacterial type II and III secretion system protein (Secretin)

(264-424)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comE Haemophilus influenzae Rd KW20

62.269

97.297

0.606
  comE Haemophilus influenzae 86-028NP

61.574

97.297

0.599
  comE Glaesserella parasuis strain SC1401

48.961

97.523

0.477
  pilQ Vibrio campbellii strain DS40M4

39.524

94.595

0.374
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

39.806

92.793

0.369
  pilQ Vibrio cholerae strain A1552

39.806

92.793

0.369