Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA/cflA   Type   Machinery gene
Locus tag   STO1_RS00175 Genome accession   NZ_AP018338
Coordinates   32895..34193 (+) Length   432 a.a.
NCBI ID   WP_096421421.1    Uniprot ID   A0A224A497
Organism   Streptococcus oralis subsp. tigurinus strain osk_001     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 27895..39193
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  STO1_RS00145 (STO1_000270) rpsB 28555..29322 (+) 768 WP_002877761.1 30S ribosomal protein S2 -
  STO1_RS00150 (STO1_000280) tsf 29401..30441 (+) 1041 WP_000808088.1 translation elongation factor Ts -
  STO1_RS00160 (STO1_000290) cysK 30721..31647 (-) 927 WP_096421417.1 cysteine synthase A -
  STO1_RS00165 (STO1_000300) - 31744..32187 (-) 444 WP_033584681.1 PH domain-containing protein -
  STO1_RS00170 (STO1_000310) - 32203..32838 (-) 636 WP_096421419.1 YigZ family protein -
  STO1_RS00175 (STO1_000320) comFA/cflA 32895..34193 (+) 1299 WP_096421421.1 DEAD/DEAH box helicase Machinery gene
  STO1_RS00180 (STO1_000330) comFC/cflB 34190..34855 (+) 666 WP_096421423.1 ComF family protein Machinery gene
  STO1_RS00185 (STO1_000340) hpf 34935..35483 (+) 549 WP_096421425.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  STO1_RS00190 (STO1_000350) - 35731..36939 (+) 1209 WP_000201480.1 acyl-CoA dehydrogenase family protein -
  STO1_RS00195 (STO1_000360) - 37020..37868 (+) 849 WP_025171502.1 3-hydroxybutyryl-CoA dehydrogenase -
  STO1_RS00200 (STO1_000370) - 37887..39065 (+) 1179 WP_007520939.1 acetyl-CoA C-acetyltransferase -

Sequence

Protein


Download         Length: 432 a.a.        Molecular weight: 49552.66 Da        Isoelectric Point: 8.5246

>NTDB_id=69027 STO1_RS00175 WP_096421421.1 32895..34193(+) (comFA/cflA) [Streptococcus oralis subsp. tigurinus strain osk_001]
MKVNPNYLGRLFTEKELTEEERQMAVKLPAMRKEKGKLLCQRCNSTILEEWYLPIGAYYCRECLLMKRVRSDQALYYFPQ
EDFPKQDVLKWRGQLTPFQEKVSEGLIRAVEKKEPTLVHAVTGAGKTEMIYQVVAKVIDEGGAVCLASPRIDVCLELYKR
LQNDFACEIALLHGESEPYFRTPLVVATTHQLLKFYHAFDLLIVDEVDAFPYVDNQMLYHTVKNSVKENGMRIFLTATST
DELDRKVRTGELKRLSLPRRFHGNPLIIPKPVWLSDFNRCLEKSQLSPKLKTYIEKQRRTGYPLLIFASEIKKGEGLKEI
LKVHFPNESIGFVSSVTEDRLEQVQAFRDGELTILISTTILERGVTFPCVDVFVVEANHRLFTKSSLIQIGGRVGRSMDR
PTGELLLFHDGLNTSIKKAIKEIKQMNMEAGL

Nucleotide


Download         Length: 1299 bp        

>NTDB_id=69027 STO1_RS00175 WP_096421421.1 32895..34193(+) (comFA/cflA) [Streptococcus oralis subsp. tigurinus strain osk_001]
ATGAAAGTAAATCCAAATTACCTCGGTCGCTTGTTTACTGAGAAAGAATTAACTGAAGAGGAACGACAGATGGCGGTGAA
ACTGCCGGCAATGAGAAAAGAGAAGGGGAAATTGCTTTGTCAACGTTGTAATAGTACGATTCTAGAGGAATGGTATCTGC
CTATCGGTGCCTACTATTGCAGGGAGTGTTTACTGATGAAGCGAGTCAGGAGTGATCAAGCTTTATACTATTTTCCGCAG
GAAGATTTTCCTAAGCAAGATGTTCTCAAATGGCGTGGTCAGTTAACCCCTTTTCAGGAAAAGGTGTCAGAGGGATTGAT
TCGAGCAGTCGAAAAGAAAGAACCGACTTTAGTTCATGCTGTAACAGGAGCTGGAAAGACGGAGATGATTTATCAGGTTG
TGGCCAAAGTGATTGATGAAGGTGGTGCAGTTTGTTTGGCCAGCCCTCGAATCGATGTGTGTTTGGAGCTGTATAAACGA
CTACAGAATGACTTTGCTTGTGAGATAGCACTACTTCATGGTGAGTCAGAACCCTATTTTCGAACACCACTAGTTGTTGC
AACAACTCATCAGTTATTAAAATTTTATCATGCTTTTGATTTGTTGATAGTGGATGAGGTAGATGCCTTTCCTTATGTTG
ACAATCAAATGCTTTACCATACTGTAAAGAATAGTGTAAAGGAGAATGGCATGAGAATCTTTTTAACAGCTACATCTACT
GATGAGTTAGATAGGAAGGTTCGCACAGGAGAATTAAAAAGATTAAGCTTGCCGAGACGGTTTCATGGAAATCCTTTGAT
TATTCCTAAACCAGTCTGGTTATCGGATTTTAATCGCTGTTTAGAGAAAAGTCAGTTATCACCTAAGTTAAAGACCTATA
TTGAGAAACAGAGAAGAACAGGTTATCCATTGCTGATTTTTGCATCAGAGATTAAGAAAGGTGAGGGACTAAAAGAAATA
CTAAAGGTGCATTTCCCGAATGAGAGCATTGGCTTTGTATCTTCTGTCACAGAAGATCGATTAGAGCAAGTACAAGCTTT
TCGAGACGGAGAACTAACAATACTGATCAGTACGACAATATTGGAACGCGGAGTTACCTTCCCTTGTGTAGATGTTTTCG
TAGTAGAAGCTAATCATCGTCTCTTTACCAAGTCTAGCTTGATTCAGATTGGTGGGCGAGTTGGGCGTAGTATGGACAGA
CCAACTGGTGAGTTGCTCCTCTTTCATGATGGATTAAATACTTCCATCAAAAAGGCAATCAAGGAAATCAAGCAGATGAA
CATGGAGGCAGGTTTATGA

Domains

Predicted by InterproScan.

DEAD/DEAH box helicase (DEAD)

(99-242)

Helicase conserved C-terminal domain (Helicase_C)

(294-396)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A224A497

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA/cflA Streptococcus mitis NCTC 12261

91.204

100

0.912
  comFA/cflA Streptococcus mitis SK321

90.509

100

0.905
  comFA/cflA Streptococcus pneumoniae Rx1

89.583

100

0.896
  comFA/cflA Streptococcus pneumoniae D39

89.583

100

0.896
  comFA/cflA Streptococcus pneumoniae R6

89.583

100

0.896
  comFA/cflA Streptococcus pneumoniae TIGR4

89.352

100

0.894
  comFA Lactococcus lactis subsp. cremoris KW2

50.856

94.676

0.481
  comFA Latilactobacillus sakei subsp. sakei 23K

38.249

100

0.384
  comFA Bacillus subtilis subsp. subtilis str. 168

39.303

93.056

0.366

Multiple sequence alignment