Detailed information NTG0690104

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: PAC42_RS02225
• Genome accession: NZ_CP097560
• Coordinates: 495426..496574 (+)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain C4.2
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 490426..501574

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PAC42_RS02200 (PAC42_02200)	-	491577..491873 (-)	297	WP_031628111.1	DUF167 domain-containing protein	-
PAC42_RS02205 (PAC42_02205)	-	491870..492463 (-)	594	WP_003084544.1	YggT family protein	-
PAC42_RS02210 (PAC42_02210)	proC	492474..493295 (-)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
PAC42_RS02215 (PAC42_02215)	-	493307..493999 (-)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
PAC42_RS02220 (PAC42_02220)	pilT	494214..495248 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
PAC42_RS02225 (PAC42_02225)	pilU	495426..496574 (+)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
PAC42_RS02230 (PAC42_02230)	czcD	496582..497481 (-)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
PAC42_RS02235 (PAC42_02235)	-	497636..498040 (+)	405	WP_003084560.1	NINE protein	-
PAC42_RS02240 (PAC42_02240)	-	498283..499656 (+)	1374	WP_015502220.1	pyridoxal-phosphate dependent enzyme	-
PAC42_RS02245 (PAC42_02245)	-	499653..500837 (+)	1185	WP_003117897.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=690104 PAC42_RS02225 WP_003100959.1 495426..496574(+) (pilU) [Pseudomonas aeruginosa strain C4.2]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=690104 PAC42_RS02225 WP_003100959.1 495426..496574(+) (pilU) [Pseudomonas aeruginosa strain C4.2]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATTACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGATTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382