Detailed information NTG0690071

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: PAC13_RS02020
• Genome accession: NZ_CP097557
• Coordinates: 427593..428741 (+)
• Length: 382 a.a.
• NCBI ID: WP_003110016.1
• Uniprot ID: A0A0H2ZL88
• Organism: Pseudomonas aeruginosa strain C1.3
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 422593..433741

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PAC13_RS01995 (PAC13_01995)	-	423744..424040 (-)	297	WP_023980267.1	DUF167 domain-containing protein	-
PAC13_RS02000 (PAC13_02000)	-	424037..424630 (-)	594	WP_003084544.1	YggT family protein	-
PAC13_RS02005 (PAC13_02005)	proC	424641..425462 (-)	822	WP_023092309.1	pyrroline-5-carboxylate reductase	-
PAC13_RS02010 (PAC13_02010)	-	425474..426166 (-)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
PAC13_RS02015 (PAC13_02015)	pilT	426381..427415 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
PAC13_RS02020 (PAC13_02020)	pilU	427593..428741 (+)	1149	WP_003110016.1	type IV pilus ATPase PilU	Machinery gene
PAC13_RS02025 (PAC13_02025)	czcD	428749..429648 (-)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
PAC13_RS02030 (PAC13_02030)	-	429803..430207 (+)	405	WP_003084560.1	NINE protein	-
PAC13_RS02035 (PAC13_02035)	-	430450..431823 (+)	1374	WP_003084562.1	pyridoxal-phosphate dependent enzyme	-
PAC13_RS02040 (PAC13_02040)	-	431820..433004 (+)	1185	WP_023092310.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42559.83 Da
• Isoelectric Point: 6.7792

>NTDB_id=690071 PAC13_RS02020 WP_003110016.1 427593..428741(+) (pilU) [Pseudomonas aeruginosa strain C1.3]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSNLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=690071 PAC13_RS02020 WP_003110016.1 427593..428741(+) (pilU) [Pseudomonas aeruginosa strain C1.3]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCATG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAACCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: A0A0H2ZL88

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.22	100	0.882
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382