Detailed information NTG0690037

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: M8Z96_RS02090
• Genome accession: NZ_CP097556
• Coordinates: 445656..446804 (+)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain B2.1
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 440656..451804

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
M8Z96_RS02065 (M8Z96_02065)	-	441807..442103 (-)	297	WP_031628111.1	DUF167 domain-containing protein	-
M8Z96_RS02070 (M8Z96_02070)	-	442100..442693 (-)	594	WP_003084544.1	YggT family protein	-
M8Z96_RS02075 (M8Z96_02075)	proC	442704..443525 (-)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
M8Z96_RS02080 (M8Z96_02080)	-	443537..444229 (-)	693	WP_015649764.1	YggS family pyridoxal phosphate-dependent enzyme	-
M8Z96_RS02085 (M8Z96_02085)	pilT	444444..445478 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
M8Z96_RS02090 (M8Z96_02090)	pilU	445656..446804 (+)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
M8Z96_RS02095 (M8Z96_02095)	czcD	446812..447711 (-)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
M8Z96_RS02100 (M8Z96_02100)	-	447865..448269 (+)	405	WP_003084560.1	NINE protein	-
M8Z96_RS02105 (M8Z96_02105)	-	448512..449885 (+)	1374	WP_003100955.1	pyridoxal-phosphate dependent enzyme	-
M8Z96_RS02110 (M8Z96_02110)	-	449882..451066 (+)	1185	WP_015649763.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=690037 M8Z96_RS02090 WP_003100959.1 445656..446804(+) (pilU) [Pseudomonas aeruginosa strain B2.1]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=690037 M8Z96_RS02090 WP_003100959.1 445656..446804(+) (pilU) [Pseudomonas aeruginosa strain B2.1]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382