Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   M6G44_RS04620 Genome accession   NZ_CP097377
Coordinates   1003687..1004133 (-) Length   148 a.a.
NCBI ID   WP_005597397.1    Uniprot ID   B0BPG5
Organism   Actinobacillus pleuropneumoniae strain GD2107     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 998687..1009133
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M6G44_RS04595 (M6G44_04595) yacG 999594..999782 (-) 189 WP_005597388.1 DNA gyrase inhibitor YacG -
  M6G44_RS04600 (M6G44_04600) coaE 999764..1000402 (-) 639 WP_005617375.1 dephospho-CoA kinase -
  M6G44_RS04605 (M6G44_04605) - 1000424..1001062 (-) 639 WP_012478451.1 prepilin peptidase -
  M6G44_RS04610 (M6G44_04610) pilC 1001089..1002291 (-) 1203 WP_005617379.1 type II secretion system F family protein Machinery gene
  M6G44_RS04615 (M6G44_04615) pilB 1002275..1003669 (-) 1395 WP_005601209.1 GspE/PulE family protein Machinery gene
  M6G44_RS04620 (M6G44_04620) pilA 1003687..1004133 (-) 447 WP_005597397.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  M6G44_RS04625 (M6G44_04625) radA 1004315..1005694 (+) 1380 WP_017357656.1 DNA repair protein RadA -
  M6G44_RS04630 (M6G44_04630) - 1005738..1005971 (-) 234 WP_005597399.1 YgjV family protein -
  M6G44_RS04635 (M6G44_04635) folA 1006098..1006586 (-) 489 WP_005597400.1 type 3 dihydrofolate reductase -
  M6G44_RS04640 (M6G44_04640) proB 1006673..1007764 (+) 1092 WP_005597401.1 glutamate 5-kinase -
  M6G44_RS04645 (M6G44_04645) - 1007777..1008553 (+) 777 WP_005597402.1 TSUP family transporter -

Sequence

Protein


Download         Length: 148 a.a.        Molecular weight: 15980.38 Da        Isoelectric Point: 7.6257

>NTDB_id=689316 M6G44_RS04620 WP_005597397.1 1003687..1004133(-) (pilA) [Actinobacillus pleuropneumoniae strain GD2107]
MQKLSLIRPLTNAFTLIELMIVIAIIAILATVAIPSYNSYTQKAALSELLAASASYKTDVEICIYNTGDSKNCSGGQNGV
RKMTELRQAKYLNAITVEGGTITVTGKGNLQEYGYTMTPIHNGSTISWETKCKGEDLSLFPANFCASN

Nucleotide


Download         Length: 447 bp        

>NTDB_id=689316 M6G44_RS04620 WP_005597397.1 1003687..1004133(-) (pilA) [Actinobacillus pleuropneumoniae strain GD2107]
ATGCAAAAATTAAGTCTTATTCGACCGCTTACTAACGCGTTTACTTTAATTGAATTGATGATCGTGATTGCGATTATTGC
CATTTTAGCTACGGTTGCAATTCCGTCATATAACAGTTATACCCAAAAAGCGGCGCTTTCGGAGCTATTGGCGGCATCGG
CTTCTTATAAAACGGATGTCGAGATCTGCATATATAACACCGGAGATTCTAAAAACTGTAGCGGCGGTCAAAACGGTGTC
AGAAAAATGACGGAGCTTAGACAGGCTAAATATTTAAATGCCATTACGGTGGAAGGCGGAACGATTACAGTAACGGGGAA
AGGGAATCTACAGGAATACGGTTATACGATGACACCGATTCATAACGGTAGCACTATTTCTTGGGAAACGAAATGTAAAG
GGGAGGACTTAAGTTTATTTCCGGCAAATTTCTGCGCATCAAATTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(13-33)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B0BPG5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Glaesserella parasuis strain SC1401

68.919

100

0.689
  pilA Haemophilus influenzae 86-028NP

55.102

99.324

0.547
  pilA Haemophilus influenzae Rd KW20

53.061

99.324

0.527