Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   M6G44_RS04610 Genome accession   NZ_CP097377
Coordinates   1001089..1002291 (-) Length   400 a.a.
NCBI ID   WP_005617379.1    Uniprot ID   B3H1L7
Organism   Actinobacillus pleuropneumoniae strain GD2107     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 996089..1007291
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M6G44_RS04585 (M6G44_04585) lptE 997889..998383 (+) 495 WP_005597385.1 LPS assembly lipoprotein LptE -
  M6G44_RS04590 (M6G44_04590) holA 998492..999517 (+) 1026 WP_005612206.1 DNA polymerase III subunit delta -
  M6G44_RS04595 (M6G44_04595) yacG 999594..999782 (-) 189 WP_005597388.1 DNA gyrase inhibitor YacG -
  M6G44_RS04600 (M6G44_04600) coaE 999764..1000402 (-) 639 WP_005617375.1 dephospho-CoA kinase -
  M6G44_RS04605 (M6G44_04605) - 1000424..1001062 (-) 639 WP_012478451.1 prepilin peptidase -
  M6G44_RS04610 (M6G44_04610) pilC 1001089..1002291 (-) 1203 WP_005617379.1 type II secretion system F family protein Machinery gene
  M6G44_RS04615 (M6G44_04615) pilB 1002275..1003669 (-) 1395 WP_005601209.1 GspE/PulE family protein Machinery gene
  M6G44_RS04620 (M6G44_04620) pilA 1003687..1004133 (-) 447 WP_005597397.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  M6G44_RS04625 (M6G44_04625) radA 1004315..1005694 (+) 1380 WP_017357656.1 DNA repair protein RadA -
  M6G44_RS04630 (M6G44_04630) - 1005738..1005971 (-) 234 WP_005597399.1 YgjV family protein -
  M6G44_RS04635 (M6G44_04635) folA 1006098..1006586 (-) 489 WP_005597400.1 type 3 dihydrofolate reductase -

Sequence

Protein


Download         Length: 400 a.a.        Molecular weight: 45594.13 Da        Isoelectric Point: 10.3238

>NTDB_id=689314 M6G44_RS04610 WP_005617379.1 1001089..1002291(-) (pilC) [Actinobacillus pleuropneumoniae strain GD2107]
MLKVYEFHWKAKNRFQQLQKGKALACHRTELERRLLAKGYSQIRIQRNFRFVTQASSESITQVIKQLALLLNAAVPLKQA
LNMLLENTYHIRIYMWLNDLNRLIESGYTMSSSLTKLNLYLAKSEIQLVRIGEQSGRLGEILQNIAVNRHQTEKLHKKIK
KIMFYPVVTLIISVALSLAMLLFIVPQFIELYSGREKPLPLITQMLFTLSLFLQHSLLQVTIVSLLAVGFFRFFAKKYRF
LTAIKNGMLSALPIFNKIVENVRIVFFSQNLGLMLKAHIRLETALNTFLATQSQDLRLQKEIENILAQLKQGYRFSESIN
ASVFGTQVVQMLAIGEQSGNVAQMCSYIGELYRQQLDDQIELLSQLLEPILMLIIGLIIGTVLIGLYLPIFDLGGLVGGG

Nucleotide


Download         Length: 1203 bp        

>NTDB_id=689314 M6G44_RS04610 WP_005617379.1 1001089..1002291(-) (pilC) [Actinobacillus pleuropneumoniae strain GD2107]
ATGTTGAAAGTGTATGAATTCCATTGGAAAGCTAAAAATCGTTTTCAACAACTTCAGAAAGGAAAAGCGTTAGCTTGTCA
TCGAACGGAATTGGAACGGCGTTTATTAGCAAAAGGCTATTCACAGATCCGTATCCAACGTAATTTTCGTTTTGTCACAC
AGGCTTCTTCAGAATCGATTACTCAAGTAATTAAACAGTTGGCGCTTTTACTGAATGCCGCAGTCCCTTTAAAACAAGCG
CTGAATATGTTGTTAGAAAATACTTATCATATTCGGATCTATATGTGGCTGAATGATTTAAACCGTTTGATAGAATCGGG
TTATACAATGTCGTCAAGTTTAACCAAATTGAATTTATATTTGGCGAAATCGGAAATACAGCTGGTACGGATTGGCGAAC
AAAGCGGGCGATTAGGCGAAATTTTGCAAAATATTGCGGTAAATCGCCATCAAACCGAAAAATTACATAAGAAAATAAAA
AAGATAATGTTTTATCCTGTCGTCACTTTGATTATTTCCGTTGCGCTTTCTCTCGCCATGCTCCTTTTTATCGTGCCGCA
ATTTATCGAGCTGTATTCCGGTCGTGAAAAGCCGCTTCCTTTAATTACACAAATGCTATTTACATTATCGCTCTTCTTAC
AACATTCTTTATTACAGGTAACGATAGTCAGTTTATTAGCGGTCGGTTTTTTCCGTTTTTTTGCAAAAAAATATCGATTT
TTAACCGCTATAAAAAACGGGATGTTAAGTGCTTTGCCTATTTTTAATAAGATAGTCGAAAACGTTCGTATAGTATTTTT
TAGTCAAAATCTCGGCTTGATGCTAAAAGCTCATATTCGGCTGGAAACGGCGTTAAATACCTTTCTTGCTACACAATCGC
AAGATTTGAGGTTACAAAAAGAGATAGAAAATATTTTAGCGCAATTAAAACAAGGTTATCGCTTTTCCGAAAGTATTAAT
GCGAGTGTGTTCGGTACTCAAGTAGTACAAATGCTTGCCATTGGCGAACAGAGTGGGAATGTAGCGCAGATGTGTAGCTA
TATCGGGGAGTTATATCGACAACAATTGGATGATCAGATTGAACTGTTATCGCAGCTGCTTGAGCCGATCTTAATGCTGA
TTATCGGATTAATTATCGGTACGGTTTTGATCGGATTATATTTACCGATTTTTGATTTAGGCGGACTAGTCGGAGGCGGT
TAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(267-389)

Type II secretion system (T2SS), protein F (T2SSF)

(64-186)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B3H1L7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Glaesserella parasuis strain SC1401

49.874

99.25

0.495
  pilC Haemophilus influenzae Rd KW20

40

100

0.4
  pilC Haemophilus influenzae 86-028NP

39

100

0.39