Detailed information    

insolico Bioinformatically predicted

Overview


Name   comGC   Type   Machinery gene
Locus tag   M5E03_RS11535 Genome accession   NZ_CP097374
Coordinates   2248534..2248827 (-) Length   97 a.a.
NCBI ID   WP_024422977.1    Uniprot ID   A0A0M2EIN6
Organism   Bacillus safensis strain AHB11     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context


Location: 2243534..2253827
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M5E03_RS11490 (M5E03_11490) sinR 2243698..2244033 (+) 336 WP_024422969.1 transcriptional regulator SinR Regulator
  M5E03_RS11495 (M5E03_11495) tasA 2244101..2244901 (-) 801 WP_034280222.1 biofilm matrix protein TasA -
  M5E03_RS11500 (M5E03_11500) sipW 2244959..2245531 (-) 573 WP_252049576.1 signal peptidase I SipW -
  M5E03_RS11505 (M5E03_11505) tapA 2245584..2246111 (-) 528 WP_041117967.1 amyloid fiber anchoring/assembly protein TapA -
  M5E03_RS11510 (M5E03_11510) - 2246411..2246689 (+) 279 WP_061109527.1 DUF3889 domain-containing protein -
  M5E03_RS11515 (M5E03_11515) - 2246724..2246918 (-) 195 WP_046311750.1 YqzE family protein -
  M5E03_RS11520 (M5E03_11520) - 2246978..2247361 (-) 384 WP_041117969.1 competence type IV pilus minor pilin ComGG -
  M5E03_RS11525 (M5E03_11525) comGF 2247358..2247885 (-) 528 WP_257788741.1 competence type IV pilus minor pilin ComGF -
  M5E03_RS19115 comGE 2247797..2248111 (-) 315 WP_041106865.1 competence type IV pilus minor pilin ComGE -
  M5E03_RS11530 (M5E03_11530) comGD 2248095..2248541 (-) 447 WP_081124572.1 competence type IV pilus minor pilin ComGD -
  M5E03_RS11535 (M5E03_11535) comGC 2248534..2248827 (-) 294 WP_024422977.1 competence type IV pilus major pilin ComGC Machinery gene
  M5E03_RS11540 (M5E03_11540) comGB 2248844..2249884 (-) 1041 WP_081124573.1 competence type IV pilus assembly protein ComGB -
  M5E03_RS11545 (M5E03_11545) comGA 2249865..2250935 (-) 1071 WP_041117979.1 competence type IV pilus ATPase ComGA Machinery gene
  M5E03_RS11550 (M5E03_11550) - 2251113..2251988 (-) 876 WP_024422979.1 STAS domain-containing protein -
  M5E03_RS11560 (M5E03_11560) - 2252212..2252592 (-) 381 WP_007501232.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  M5E03_RS11565 (M5E03_11565) - 2252805..2253047 (+) 243 WP_003217440.1 DUF2626 domain-containing protein -
  M5E03_RS11570 (M5E03_11570) - 2253093..2253731 (-) 639 WP_070326499.1 MBL fold metallo-hydrolase -

Sequence


Protein


Download         Length: 97 a.a.        Molecular weight: 10686.62 Da        Isoelectric Point: 7.3218

>NTDB_id=689266 M5E03_RS11535 WP_024422977.1 2248534..2248827(-) (comGC) [Bacillus safensis strain AHB11]
MNDKGFTLLEMLIVMLVISILLLITIPNITRHHQAIQAKGCEGLKSMVSTQVMAYELEHDGKTPSLAELEKEGYVKKGLT
CPNGKAIVIQNGNVKHE

Nucleotide


Download         Length: 294 bp        

>NTDB_id=689266 M5E03_RS11535 WP_024422977.1 2248534..2248827(-) (comGC) [Bacillus safensis strain AHB11]
ATGAATGATAAAGGATTTACCCTGTTAGAAATGTTAATAGTCATGCTTGTAATATCCATTCTTTTACTCATTACCATACC
GAATATTACACGTCATCACCAAGCTATTCAGGCAAAGGGCTGTGAAGGGCTTAAATCAATGGTCAGCACACAAGTGATGG
CATATGAATTGGAGCATGATGGGAAGACGCCATCTTTAGCTGAATTAGAAAAAGAAGGTTATGTCAAAAAAGGATTAACT
TGTCCTAATGGAAAAGCGATTGTCATTCAAAACGGCAACGTGAAGCATGAATAA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure
  AlphaFold DB A0A0M2EIN6

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGC Bacillus subtilis subsp. subtilis str. 168

67.01

100

0.67

  comGC Staphylococcus aureus MW2

42.222

92.784

0.392

  comGC Staphylococcus aureus N315

42.222

92.784

0.392