Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   M5E03_RS11545 Genome accession   NZ_CP097374
Coordinates   2249865..2250935 (-) Length   356 a.a.
NCBI ID   WP_041117979.1    Uniprot ID   -
Organism   Bacillus safensis strain AHB11     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2244865..2255935
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M5E03_RS11500 (M5E03_11500) sipW 2244959..2245531 (-) 573 WP_252049576.1 signal peptidase I SipW -
  M5E03_RS11505 (M5E03_11505) tapA 2245584..2246111 (-) 528 WP_041117967.1 amyloid fiber anchoring/assembly protein TapA -
  M5E03_RS11510 (M5E03_11510) - 2246411..2246689 (+) 279 WP_061109527.1 DUF3889 domain-containing protein -
  M5E03_RS11515 (M5E03_11515) - 2246724..2246918 (-) 195 WP_046311750.1 YqzE family protein -
  M5E03_RS11520 (M5E03_11520) - 2246978..2247361 (-) 384 WP_041117969.1 competence type IV pilus minor pilin ComGG -
  M5E03_RS11525 (M5E03_11525) comGF 2247358..2247885 (-) 528 WP_257788741.1 competence type IV pilus minor pilin ComGF -
  M5E03_RS19115 comGE 2247797..2248111 (-) 315 WP_041106865.1 competence type IV pilus minor pilin ComGE -
  M5E03_RS11530 (M5E03_11530) comGD 2248095..2248541 (-) 447 WP_081124572.1 competence type IV pilus minor pilin ComGD -
  M5E03_RS11535 (M5E03_11535) comGC 2248534..2248827 (-) 294 WP_024422977.1 competence type IV pilus major pilin ComGC Machinery gene
  M5E03_RS11540 (M5E03_11540) comGB 2248844..2249884 (-) 1041 WP_081124573.1 competence type IV pilus assembly protein ComGB -
  M5E03_RS11545 (M5E03_11545) comGA 2249865..2250935 (-) 1071 WP_041117979.1 competence type IV pilus ATPase ComGA Machinery gene
  M5E03_RS11550 (M5E03_11550) - 2251113..2251988 (-) 876 WP_024422979.1 STAS domain-containing protein -
  M5E03_RS11560 (M5E03_11560) - 2252212..2252592 (-) 381 WP_007501232.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  M5E03_RS11565 (M5E03_11565) - 2252805..2253047 (+) 243 WP_003217440.1 DUF2626 domain-containing protein -
  M5E03_RS11570 (M5E03_11570) - 2253093..2253731 (-) 639 WP_070326499.1 MBL fold metallo-hydrolase -
  M5E03_RS11575 (M5E03_11575) - 2253889..2254062 (+) 174 WP_003217333.1 DUF2759 domain-containing protein -
  M5E03_RS11580 (M5E03_11580) - 2254086..2254400 (-) 315 WP_024427984.1 MTH1187 family thiamine-binding protein -
  M5E03_RS11585 (M5E03_11585) - 2254477..2255730 (-) 1254 WP_252049578.1 MFS transporter -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40398.79 Da        Isoelectric Point: 9.4061

>NTDB_id=689267 M5E03_RS11545 WP_041117979.1 2249865..2250935(-) (comGA) [Bacillus safensis strain AHB11]
MYGIEYLRQELLEEACRMRASDVHIVPKEKEASVSFRVDSDLIQQRTIDKKSGERLIAHFKFLSSMDIGEKRRPQNGSLA
VMLRNGQVFIRMSTLPTVNDESLVIRILPQDHVPKIKHLSLFPKASARLLSFLNHSHGLILFTGPTNSGKTTTLYSLIQF
AKKNFNRNIITLEDPVETRNEEVLQVQVNEKAGITYAAGLRAILRHDPDVIVLGEIRDAETARTAIRAALTGHLVLSTLH
AKNAKGALYRMLEFGVTMNELEQTMVAIAAQRLIELTCPFCGERCQLYCKLNRPIRRTNVFELLFGKELGECIKEAKGEY
AHSSYETLQRLIRKGVALGYLSKNTYHRWVYEEASL

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=689267 M5E03_RS11545 WP_041117979.1 2249865..2250935(-) (comGA) [Bacillus safensis strain AHB11]
TTGTATGGGATTGAATATTTAAGGCAAGAGCTGCTAGAAGAAGCATGCCGCATGAGGGCTTCTGATGTGCATATTGTGCC
AAAAGAAAAGGAAGCTTCGGTGTCATTTCGTGTTGATTCAGATTTAATCCAGCAGCGAACGATTGATAAAAAAAGCGGAG
AGAGGCTTATTGCACATTTTAAGTTTTTATCTTCTATGGATATCGGGGAAAAAAGGAGGCCGCAAAATGGCTCGCTAGCT
GTAATGCTAAGAAACGGTCAAGTGTTTATTCGGATGTCTACCTTGCCGACAGTGAATGATGAGAGCTTAGTGATTAGAAT
TTTACCGCAGGATCATGTTCCGAAAATAAAACATCTATCATTATTTCCGAAAGCCTCAGCCAGATTATTATCCTTTTTAA
ACCACTCGCATGGACTCATTTTATTTACTGGGCCAACAAATTCAGGGAAAACAACAACACTATATTCATTGATCCAGTTT
GCAAAAAAGAATTTTAACCGAAATATTATTACACTTGAAGATCCTGTCGAAACAAGAAATGAAGAAGTGCTGCAGGTTCA
AGTGAATGAAAAAGCCGGCATCACGTATGCCGCTGGATTACGCGCTATTTTAAGACATGATCCAGATGTGATTGTGCTAG
GAGAAATAAGAGATGCTGAAACGGCTAGAACGGCGATTAGAGCAGCACTGACAGGCCATTTAGTATTGAGTACGCTCCAT
GCAAAAAATGCAAAAGGAGCCCTTTACCGCATGCTTGAATTTGGTGTGACGATGAATGAACTTGAACAAACCATGGTTGC
GATTGCTGCCCAGCGATTAATCGAGCTCACCTGTCCTTTTTGCGGAGAAAGATGTCAGCTTTACTGTAAATTAAATCGAC
CGATCAGACGAACAAATGTATTTGAGCTGCTGTTCGGAAAAGAGCTTGGTGAGTGTATCAAGGAGGCTAAAGGAGAATAT
GCTCACTCGTCATATGAAACACTGCAAAGATTAATTCGTAAAGGAGTGGCACTCGGCTATTTATCAAAAAACACTTACCA
TCGCTGGGTTTATGAAGAAGCGAGCCTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

64.689

99.438

0.643