Detailed information NTG0689189

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: OSV60_RS13970
• Genome accession: NZ_CP113077
• Coordinates: 2971967..2973085 (+)
• Length: 372 a.a.
• NCBI ID: WP_000347036.1
• Uniprot ID: A0A086HYC5
• Organism: Acinetobacter baumannii strain 86II/2C
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2966967..2978085

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
OSV60_RS13960 (OSV60_13960)	-	2970091..2970783 (-)	693	WP_047479993.1	YggS family pyridoxal phosphate-dependent enzyme	-
OSV60_RS13965 (OSV60_13965)	pilT	2970910..2971947 (+)	1038	WP_000355489.1	type IV pilus twitching motility protein PilT	Machinery gene
OSV60_RS13970 (OSV60_13970)	pilU	2971967..2973085 (+)	1119	WP_000347036.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
OSV60_RS13975 (OSV60_13975)	fur	2973155..2973592 (-)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
OSV60_RS13980 (OSV60_13980)	-	2973704..2974102 (+)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
OSV60_RS13985 (OSV60_13985)	-	2974157..2974480 (-)	324	WP_098713869.1	RnfH family protein	-
OSV60_RS13990 (OSV60_13990)	-	2974480..2975547 (-)	1068	WP_000092403.1	hypothetical protein	-
OSV60_RS13995 (OSV60_13995)	-	2975700..2976155 (-)	456	WP_000782976.1	bacteriohemerythrin	-
OSV60_RS14000 (OSV60_14000)	-	2976408..2977256 (-)	849	WP_000336551.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41905.11 Da
• Isoelectric Point: 6.6944

>NTDB_id=689189 OSV60_RS13970 WP_000347036.1 2971967..2973085(+) (pilU) [Acinetobacter baumannii strain 86II/2C]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRKEFAETRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=689189 OSV60_RS13970 WP_000347036.1 2971967..2973085(+) (pilU) [Acinetobacter baumannii strain 86II/2C]
ATGGATTTTAATGACCTGCTCAACCTCATGGTTGAAAAAAAATCATCCGATCTCTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAACAGTCTTTCTGGGGAAGTGATTGGTCAACTGTTACATTCCA
TCATGAGTGAAAAACAACGCAAAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCAGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGCGGCATTATTATTTTCGTAGGCGCGACAG
GTACAGGTAAATCCACTTCATTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGCTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGCCATCTTGTATTAGCTACCATGCACGCTAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGACCGTCATAGTCAGCTTTATATGGACTTGTCACTTAACTTAAAGGCAATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAAGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCTCTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCTGATGAAGGTCCCGATCAATTATCAGACGGGAAACAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterProScan.

• Type II/IV secretion system protein (T2SSE) (31-270)

3D structure

• AlphaFold DB: A0A086HYC5

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.926	100	0.772
pilU	Pseudomonas stutzeri DSM 10701	69.293	98.925	0.685
pilU	Vibrio cholerae strain A1552	53.243	99.462	0.53
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.197	93.011	0.392