Detailed information    

insolico Bioinformatically predicted

Overview

Name   comE   Type   Machinery gene
Locus tag   M3703_RS03765 Genome accession   NZ_CP097340
Coordinates   718169..719482 (-) Length   437 a.a.
NCBI ID   WP_249762557.1    Uniprot ID   -
Organism   Mannheimia haemolytica strain B-31     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 713169..724482
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M3703_RS03735 (M3703_03735) ruvB 713666..714670 (-) 1005 WP_040080998.1 Holliday junction branch migration DNA helicase RuvB -
  M3703_RS03740 (M3703_03740) ruvA 714807..715412 (-) 606 WP_006247943.1 Holliday junction branch migration protein RuvA -
  M3703_RS03745 (M3703_03745) - 715524..716147 (-) 624 WP_006247941.1 LysE family transporter -
  M3703_RS03750 (M3703_03750) - 716154..716627 (-) 474 WP_006247940.1 phosphatidylglycerophosphatase A -
  M3703_RS03755 (M3703_03755) thiL 716632..717612 (-) 981 WP_006247939.1 thiamine-phosphate kinase -
  M3703_RS03760 (M3703_03760) nusB 717675..718088 (-) 414 WP_006247938.1 transcription antitermination factor NusB -
  M3703_RS03765 (M3703_03765) comE 718169..719482 (-) 1314 WP_249762557.1 type IV pilus secretin PilQ Machinery gene
  M3703_RS03770 (M3703_03770) - 719491..719904 (-) 414 WP_006247936.1 hypothetical protein -
  M3703_RS03775 (M3703_03775) - 719907..720443 (-) 537 WP_006247935.1 hypothetical protein -
  M3703_RS03780 (M3703_03780) - 720445..720966 (-) 522 WP_006247934.1 hypothetical protein -
  M3703_RS03785 (M3703_03785) - 720942..721640 (-) 699 WP_006247933.1 hypothetical protein -
  M3703_RS03790 (M3703_03790) - 721764..724328 (+) 2565 WP_044426683.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 437 a.a.        Molecular weight: 48547.57 Da        Isoelectric Point: 8.0673

>NTDB_id=688784 M3703_RS03765 WP_249762557.1 718169..719482(-) (comE) [Mannheimia haemolytica strain B-31]
MWRIFIYFLCGFSLAYANTISIVLKNAPTSLIFTYLAEETGKNMVLDDNIETKSTLRLENKSVDEIFKTLGKVNKLSLTQ
EDDIVYIHKKEEKAADLTPLPIMNLQNNGQNTPLVTAPKLITKTLKLHYAKASEVIESLTKGSGTFLSENGYIHFDERSN
SLIVKDSAKSLKNIENLVKQLDQPTEQIAIEARIVTISSEHLQELGVRWGIFSRGAGHYKFGGRLEGNGLNDVTNNLNVN
FPITGGTSAVLQVASINSRVLDLELSALEQENSVEIIASPRLLTTNKKPASIKQGTEIPYVMYNTKSEATDVEFKEAVLG
LEVVPHLSTQNQILLDLIVTQNSPNSQSGSSGLITIDKQELNTQVFAKHGETIVLGSIFQHLTQKGEDKVPILGSIPFIK
RLFSQTRDKISKRELVIFVTPYILQSNEKSSNRKKSK

Nucleotide


Download         Length: 1314 bp        

>NTDB_id=688784 M3703_RS03765 WP_249762557.1 718169..719482(-) (comE) [Mannheimia haemolytica strain B-31]
ATGTGGCGAATTTTTATATATTTCTTATGCGGTTTTTCATTAGCCTATGCGAATACCATTTCCATAGTTTTAAAAAATGC
TCCAACTTCGCTTATTTTTACTTATTTAGCGGAAGAAACCGGTAAAAATATGGTGCTTGATGATAATATTGAAACCAAAT
CAACACTACGATTAGAAAATAAATCGGTTGACGAGATTTTTAAAACGCTTGGCAAAGTCAATAAATTATCCTTAACGCAA
GAAGATGATATTGTTTATATTCACAAAAAAGAGGAAAAAGCGGCTGACTTAACGCCTCTGCCAATAATGAATTTGCAAAA
TAATGGGCAAAATACACCGCTTGTCACCGCCCCTAAGTTAATCACAAAAACCTTGAAACTACATTATGCAAAGGCTTCAG
AGGTGATTGAGTCGTTAACCAAAGGCAGCGGAACGTTTTTATCGGAAAACGGTTATATTCACTTTGATGAAAGAAGCAAT
AGCCTGATTGTGAAAGACAGTGCAAAATCGCTTAAAAATATCGAGAATTTAGTAAAACAGCTCGACCAACCCACCGAGCA
AATTGCGATTGAGGCTAGAATTGTCACTATCAGTAGCGAGCATTTGCAAGAGCTTGGCGTTCGCTGGGGAATTTTTTCTC
GTGGGGCTGGGCACTATAAATTTGGCGGACGGCTGGAAGGCAATGGACTCAACGATGTCACCAATAACTTAAATGTAAAT
TTTCCGATTACTGGTGGAACATCTGCCGTATTACAGGTTGCGTCCATTAATAGCAGAGTGCTTGATTTAGAATTAAGTGC
ATTAGAACAAGAAAATAGTGTGGAAATTATTGCCAGCCCACGCCTGTTAACCACCAATAAAAAGCCGGCAAGTATCAAGC
AAGGCACGGAAATTCCTTATGTGATGTACAACACCAAATCGGAAGCCACCGATGTCGAATTTAAAGAAGCGGTATTAGGG
CTTGAAGTCGTTCCACACCTTTCAACCCAAAATCAAATTCTACTCGATCTGATCGTTACCCAAAATTCGCCAAATTCCCA
ATCCGGCAGTAGTGGCTTAATTACGATTGATAAACAAGAGCTAAATACGCAAGTATTTGCCAAACACGGCGAAACCATTG
TACTTGGTAGTATTTTTCAACATTTAACCCAAAAAGGCGAAGATAAAGTGCCGATTTTAGGCTCAATTCCCTTCATCAAG
CGGTTATTTAGTCAAACTCGAGATAAAATCAGCAAAAGAGAGCTAGTCATTTTTGTGACGCCTTATATCCTTCAATCTAA
TGAAAAGAGCAGCAACCGCAAAAAATCAAAATAG

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(122-187)

Bacterial type II and III secretion system protein (Secretin)

(267-424)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comE Glaesserella parasuis strain SC1401

62.615

99.771

0.625
  comE Haemophilus influenzae Rd KW20

50.805

99.542

0.506
  comE Haemophilus influenzae 86-028NP

50.115

99.542

0.499
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

38.955

96.339

0.375
  pilQ Vibrio cholerae strain A1552

38.955

96.339

0.375
  pilQ Vibrio campbellii strain DS40M4

37.355

98.627

0.368