Detailed information    

insolico Bioinformatically predicted

Overview

Name   comE   Type   Machinery gene
Locus tag   M3710_RS02355 Genome accession   NZ_CP097339
Coordinates   457451..458764 (-) Length   437 a.a.
NCBI ID   WP_249762237.1    Uniprot ID   -
Organism   Mannheimia haemolytica strain H-5106     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 452451..463764
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M3710_RS02325 (M3710_02325) ruvB 452948..453952 (-) 1005 WP_006247944.1 Holliday junction branch migration DNA helicase RuvB -
  M3710_RS02330 (M3710_02330) ruvA 454089..454694 (-) 606 WP_006247943.1 Holliday junction branch migration protein RuvA -
  M3710_RS02335 (M3710_02335) - 454806..455429 (-) 624 WP_006247941.1 LysE family transporter -
  M3710_RS02340 (M3710_02340) - 455436..455909 (-) 474 WP_006247940.1 phosphatidylglycerophosphatase A -
  M3710_RS02345 (M3710_02345) thiL 455914..456894 (-) 981 WP_006247939.1 thiamine-phosphate kinase -
  M3710_RS02350 (M3710_02350) nusB 456957..457370 (-) 414 WP_006247938.1 transcription antitermination factor NusB -
  M3710_RS02355 (M3710_02355) comE 457451..458764 (-) 1314 WP_249762237.1 type IV pilus secretin PilQ Machinery gene
  M3710_RS02360 (M3710_02360) - 458773..459186 (-) 414 WP_006247936.1 hypothetical protein -
  M3710_RS02365 (M3710_02365) - 459189..459725 (-) 537 WP_249762238.1 hypothetical protein -
  M3710_RS02370 (M3710_02370) - 459727..460248 (-) 522 WP_006247934.1 hypothetical protein -
  M3710_RS02375 (M3710_02375) - 460224..460922 (-) 699 WP_006247933.1 hypothetical protein -
  M3710_RS02380 (M3710_02380) - 461046..463610 (+) 2565 WP_006247932.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 437 a.a.        Molecular weight: 48501.59 Da        Isoelectric Point: 9.2350

>NTDB_id=688759 M3710_RS02355 WP_249762237.1 457451..458764(-) (comE) [Mannheimia haemolytica strain H-5106]
MWRIFIYFLCGFSLAYANTISIVLKNAPTSLIFTYLAEETGKNMVLDDNIETKSTLRLENKSVDEIFKTLGKVNKLSLTQ
EDDIVYIHKKEKKAADLTPLPIMNLQNNGQNTPLVTAPKLITKTLKLHYAKASEVIESLTKGSGTFLSENGYIHFDERSN
SLIVKDSAKSLKNIENLVKQLDQPTEQIAIEARIVTISSEHLQELGVRWGIFSRGAGHYKFGGRLEGNGLNNVTNNLNVN
FPITGGASAVLQVSSINSRVLDLELSALEQENSVEIIASPRLLTTNKKPASIKQGTEIPYVMYNTKSEATDVEFKEAVLG
LEVVPHLSTQNQILLDLIVTQNSPNSQSGSSGLITIDKQELNTQVFAKHGETIVLGGIFQHLTQKGEDKVPILGSIPFIK
RLFSQTRDKISKRELVIFVTPYILQSNEKSSNRKKSK

Nucleotide


Download         Length: 1314 bp        

>NTDB_id=688759 M3710_RS02355 WP_249762237.1 457451..458764(-) (comE) [Mannheimia haemolytica strain H-5106]
ATGTGGCGAATTTTTATATATTTCTTATGCGGTTTTTCATTAGCCTATGCGAATACCATTTCCATAGTTTTAAAAAATGC
TCCAACTTCGCTTATTTTTACTTATTTAGCGGAAGAAACCGGTAAAAATATGGTGCTTGATGATAATATTGAAACCAAAT
CAACACTACGATTAGAAAATAAATCGGTTGACGAGATTTTTAAAACGCTTGGCAAAGTCAATAAATTATCCTTAACGCAA
GAAGATGATATTGTTTATATTCACAAAAAAGAGAAAAAAGCGGCTGACTTAACGCCTCTGCCAATAATGAATTTGCAAAA
TAATGGGCAAAATACACCGCTTGTCACCGCCCCTAAGTTAATCACAAAAACCTTGAAACTACATTATGCAAAGGCTTCAG
AGGTGATTGAGTCGTTAACCAAAGGCAGCGGAACGTTTTTATCGGAAAACGGTTATATTCACTTTGATGAAAGAAGCAAT
AGCCTGATTGTGAAAGACAGTGCAAAATCGCTTAAAAATATCGAGAATTTAGTAAAACAGCTCGACCAACCCACCGAGCA
AATTGCGATTGAGGCTAGAATTGTCACTATCAGTAGCGAGCATTTGCAAGAGCTTGGCGTTCGCTGGGGAATTTTTTCTC
GTGGGGCTGGGCACTATAAATTTGGCGGACGGCTGGAAGGCAATGGACTCAACAATGTCACCAATAACTTAAATGTAAAT
TTTCCGATTACTGGTGGAGCATCTGCCGTATTACAGGTTTCGTCCATTAATAGCAGAGTGCTTGATTTAGAATTAAGTGC
ATTAGAACAAGAAAATAGTGTGGAAATTATTGCCAGCCCACGCCTGTTAACCACCAATAAAAAGCCGGCAAGTATCAAGC
AAGGCACGGAAATTCCTTATGTGATGTACAACACCAAATCGGAAGCCACCGATGTCGAATTTAAAGAAGCGGTATTAGGG
CTTGAAGTCGTTCCACACCTTTCAACCCAAAATCAAATTCTACTCGATCTGATCGTTACCCAAAATTCGCCAAATTCCCA
ATCCGGCAGTAGTGGCTTAATTACGATTGATAAACAAGAGCTAAATACGCAAGTATTTGCCAAACACGGCGAAACCATTG
TACTTGGTGGTATTTTTCAACATTTAACCCAAAAAGGCGAAGATAAAGTGCCGATTTTAGGCTCAATTCCCTTCATCAAG
CGGTTATTTAGTCAAACTCGAGATAAAATCAGCAAAAGAGAGCTAGTCATTTTTGTGACGCCTTATATCCTTCAATCTAA
TGAAAAGAGCAGCAACCGCAAAAAATCAAAATAG

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(267-424)

Bacterial type II/III secretion system short domain (Secretin_N)

(122-187)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comE Glaesserella parasuis strain SC1401

62.615

99.771

0.625
  comE Haemophilus influenzae Rd KW20

51.034

99.542

0.508
  comE Haemophilus influenzae 86-028NP

50.345

99.542

0.501
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

38.48

96.339

0.371
  pilQ Vibrio cholerae strain A1552

38.48

96.339

0.371
  pilQ Vibrio campbellii strain DS40M4

37.15

97.941

0.364