Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   M3709_RS04560 Genome accession   NZ_CP097333
Coordinates   892757..893953 (+) Length   398 a.a.
NCBI ID   WP_006248403.1    Uniprot ID   A0A249A0I8
Organism   Mannheimia haemolytica strain 184     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 887757..898953
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M3709_RS04535 (M3709_04535) folA 888414..888908 (+) 495 WP_006248397.1 type 3 dihydrofolate reductase -
  M3709_RS04540 (M3709_04540) - 889028..889258 (+) 231 WP_006248398.1 YgjV family protein -
  M3709_RS04545 (M3709_04545) radA 889313..890692 (-) 1380 WP_040080753.1 DNA repair protein RadA Machinery gene
  M3709_RS04550 (M3709_04550) pilA 890873..891352 (+) 480 WP_006248401.1 prepilin peptidase-dependent pilin Machinery gene
  M3709_RS04555 (M3709_04555) pilB 891373..892773 (+) 1401 WP_040080755.1 GspE/PulE family protein Machinery gene
  M3709_RS04560 (M3709_04560) pilC 892757..893953 (+) 1197 WP_006248403.1 type II secretion system F family protein Machinery gene
  M3709_RS04565 (M3709_04565) - 893956..894612 (+) 657 WP_006248404.1 prepilin peptidase -
  M3709_RS04570 (M3709_04570) coaE 894648..895283 (+) 636 WP_020831305.1 dephospho-CoA kinase -
  M3709_RS04575 (M3709_04575) yacG 895265..895450 (+) 186 WP_006248406.1 DNA gyrase inhibitor YacG -
  M3709_RS04580 (M3709_04580) holA 895451..896476 (-) 1026 WP_006253426.1 DNA polymerase III subunit delta -
  M3709_RS04585 (M3709_04585) lptE 896519..897007 (-) 489 WP_006251337.1 LPS assembly lipoprotein LptE -

Sequence

Protein


Download         Length: 398 a.a.        Molecular weight: 45186.75 Da        Isoelectric Point: 9.4698

>NTDB_id=688649 M3709_RS04560 WP_006248403.1 892757..893953(+) (pilC) [Mannheimia haemolytica strain 184]
MTKIYEFHWQAQSRFGQKQRGKRLATNRDMLENLLLAQGYQHIRISRNFVLSANPKKEEVNSLLSQLALLVNSAMPLKQA
LSMLLQNCRNIRLYLWLNELIQHIESGYAFSQSLEKLGEFVTNQEIQLIKMGEQSGKLGIMLTNLAESRAKLEKLAKKVK
KILFYPVIVLLISLALSIGLLVLIVPQFAQLYGSKDKTLPLITDILFTLSECLIEQGTVLLGTLLLAVIFYLFFAKKSRL
VAKWKMALWAKMPLFSRIIEQSRIIFFSQNIALMLNAHIPLDVALRSFLSEKNDDPILQQEIGTMLNLLQQGYKFSQGIN
PNVFGAEMAQMLEVGEKSGNVAKMCEHISEMYQQKLDYQIDILSQLLEPMLMLIMGVIVGTIIVGLYLPIFDMGALVE

Nucleotide


Download         Length: 1197 bp        

>NTDB_id=688649 M3709_RS04560 WP_006248403.1 892757..893953(+) (pilC) [Mannheimia haemolytica strain 184]
ATGACGAAAATCTATGAATTTCATTGGCAAGCCCAGAGCCGATTTGGGCAAAAACAGCGGGGCAAGCGGCTGGCAACGAA
TCGAGACATGTTGGAAAACCTTTTGCTGGCACAAGGTTATCAGCATATTCGGATTAGCCGAAATTTTGTGCTTTCAGCAA
ACCCGAAAAAAGAAGAGGTGAATAGCTTGCTATCACAGCTTGCATTGTTGGTTAATTCTGCCATGCCGTTAAAACAGGCA
TTATCTATGTTGTTGCAAAACTGCCGAAATATCCGCCTTTATCTATGGCTTAATGAGCTGATTCAGCATATTGAAAGTGG
CTATGCTTTTTCACAATCGTTGGAGAAATTGGGTGAATTTGTGACCAATCAGGAAATTCAGCTAATCAAAATGGGCGAAC
AAAGCGGAAAACTGGGCATTATGCTGACTAATTTAGCTGAAAGCCGAGCGAAATTGGAAAAATTGGCGAAAAAAGTGAAG
AAAATCCTGTTCTACCCGGTGATTGTGTTGCTGATTTCGCTCGCTTTATCTATCGGGCTATTGGTTTTGATTGTACCGCA
GTTTGCCCAACTTTATGGCTCAAAAGACAAAACTTTACCCCTCATTACCGATATTTTATTTACCCTTTCCGAATGCTTGA
TTGAGCAAGGCACAGTATTACTTGGCACGTTACTACTAGCGGTCATTTTTTACCTGTTTTTTGCAAAAAAGAGCAGGTTA
GTCGCAAAGTGGAAAATGGCTCTATGGGCTAAAATGCCACTGTTTAGCCGAATTATTGAGCAATCTCGCATTATCTTTTT
CAGCCAAAATATTGCCTTAATGCTGAATGCTCATATTCCGCTTGATGTTGCCTTGAGGTCGTTTTTATCCGAGAAAAATG
ATGACCCGATTTTACAGCAAGAAATTGGCACTATGCTTAATTTGTTACAACAAGGCTACAAATTTTCACAAGGGATCAAC
CCGAATGTGTTTGGTGCTGAGATGGCACAAATGCTTGAGGTTGGGGAGAAAAGTGGCAATGTGGCGAAAATGTGTGAGCA
TATTAGTGAGATGTACCAACAAAAATTAGATTACCAAATTGATATTTTGTCTCAGCTATTAGAACCGATGCTAATGTTGA
TAATGGGTGTGATTGTCGGCACTATTATTGTTGGGCTGTATTTACCGATTTTTGATATGGGGGCTTTGGTGGAGTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(64-186)

Type II secretion system (T2SS), protein F (T2SSF)

(267-389)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A249A0I8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Glaesserella parasuis strain SC1401

54

100

0.543
  pilC Haemophilus influenzae Rd KW20

42.145

100

0.425
  pilC Haemophilus influenzae 86-028NP

41.147

100

0.415