Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   M3709_RS04550 Genome accession   NZ_CP097333
Coordinates   890873..891352 (+) Length   159 a.a.
NCBI ID   WP_006248401.1    Uniprot ID   A0A249A0S4
Organism   Mannheimia haemolytica strain 184     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 885873..896352
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M3709_RS04525 (M3709_04525) - 886447..887211 (-) 765 WP_006248395.1 TSUP family transporter -
  M3709_RS04530 (M3709_04530) proB 887211..888302 (-) 1092 WP_006251346.1 glutamate 5-kinase -
  M3709_RS04535 (M3709_04535) folA 888414..888908 (+) 495 WP_006248397.1 type 3 dihydrofolate reductase -
  M3709_RS04540 (M3709_04540) - 889028..889258 (+) 231 WP_006248398.1 YgjV family protein -
  M3709_RS04545 (M3709_04545) radA 889313..890692 (-) 1380 WP_040080753.1 DNA repair protein RadA Machinery gene
  M3709_RS04550 (M3709_04550) pilA 890873..891352 (+) 480 WP_006248401.1 prepilin peptidase-dependent pilin Machinery gene
  M3709_RS04555 (M3709_04555) pilB 891373..892773 (+) 1401 WP_040080755.1 GspE/PulE family protein Machinery gene
  M3709_RS04560 (M3709_04560) pilC 892757..893953 (+) 1197 WP_006248403.1 type II secretion system F family protein Machinery gene
  M3709_RS04565 (M3709_04565) - 893956..894612 (+) 657 WP_006248404.1 prepilin peptidase -
  M3709_RS04570 (M3709_04570) coaE 894648..895283 (+) 636 WP_020831305.1 dephospho-CoA kinase -
  M3709_RS04575 (M3709_04575) yacG 895265..895450 (+) 186 WP_006248406.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 159 a.a.        Molecular weight: 16841.15 Da        Isoelectric Point: 8.7964

>NTDB_id=688647 M3709_RS04550 WP_006248401.1 890873..891352(+) (pilA) [Mannheimia haemolytica strain 184]
MQKIVHFRPLGIRQLHRAFTLIELMIVIAIIAILATIAIPSYNSYTQKAALSELLQASASYKSDVEVCIYNSGGLDNCSG
GTNGIQANKTSSNTTKYLNSIAVTKGVITVTGKGSLNGYGYTLTPSFSNNTISWTASCSGNDTSLFPANFCSTTVTPNG

Nucleotide


Download         Length: 480 bp        

>NTDB_id=688647 M3709_RS04550 WP_006248401.1 890873..891352(+) (pilA) [Mannheimia haemolytica strain 184]
ATGCAAAAAATTGTACATTTTAGACCGCTTGGCATTCGCCAACTGCATCGGGCATTTACCCTCATTGAGTTGATGATCGT
GATTGCGATTATTGCCATTCTTGCCACTATTGCGATCCCTTCCTATAACTCATACACCCAAAAGGCGGCGTTATCGGAGT
TATTACAAGCATCTGCTTCGTATAAATCAGACGTTGAAGTCTGTATTTACAACAGTGGCGGGTTGGATAACTGCTCCGGT
GGCACGAATGGGATTCAGGCGAATAAAACCAGTAGTAATACCACTAAATACCTAAACTCAATTGCGGTAACAAAAGGCGT
GATTACGGTAACCGGCAAAGGCTCTCTGAATGGTTATGGCTATACCTTAACACCGTCGTTCTCAAATAATACGATTAGTT
GGACGGCAAGTTGTAGTGGCAATGATACGAGCCTATTTCCAGCTAATTTCTGTAGCACAACTGTTACACCAAACGGCTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(48-148)

Prokaryotic N-terminal methylation motif (N_methyl)

(16-38)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A249A0S4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Glaesserella parasuis strain SC1401

66.038

100

0.66
  pilA Haemophilus influenzae 86-028NP

56.429

88.05

0.497
  pilA Haemophilus influenzae Rd KW20

54.93

89.308

0.491