Detailed information NTG0687518

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: M3M30_RS08815
• Genome accession: NZ_CP097161
• Coordinates: 1935954..1937024 (-)
• Length: 356 a.a.
• NCBI ID: WP_274517079.1
• Uniprot ID: -
• Organism: Methylococcus capsulatus strain MIR
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1930954..1942024

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
M3M30_RS08790 (M3M30_08790)	aroK	1931395..1931934 (-)	540	WP_010959691.1	shikimate kinase AroK	-
M3M30_RS08795 (M3M30_08795)	pilQ	1931996..1934056 (-)	2061	WP_249385481.1	type IV pilus secretin PilQ	-
M3M30_RS08800 (M3M30_08800)	-	1934172..1934714 (-)	543	WP_010959689.1	pilus assembly protein PilP	-
M3M30_RS08805 (M3M30_08805)	-	1934714..1935352 (-)	639	WP_010959688.1	type 4a pilus biogenesis protein PilO	-
M3M30_RS08810 (M3M30_08810)	-	1935349..1935954 (-)	606	WP_017365266.1	PilN domain-containing protein	-
M3M30_RS08815 (M3M30_08815)	pilM	1935954..1937024 (-)	1071	WP_274517079.1	pilus assembly protein PilM	Machinery gene
M3M30_RS08820 (M3M30_08820)	-	1937337..1939784 (+)	2448	WP_249385139.1	penicillin-binding protein 1A	-
M3M30_RS08825 (M3M30_08825)	-	1939800..1940411 (+)	612	WP_249385140.1	hypothetical protein	-
M3M30_RS08830 (M3M30_08830)	rpmE	1940447..1940650 (-)	204	WP_010959683.1	50S ribosomal protein L31	-
M3M30_RS08835 (M3M30_08835)	-	1940765..1941538 (+)	774	WP_010959682.1	exodeoxyribonuclease III	-

Sequence

Protein

• Length: 356 a.a.
• Molecular weight: 38715.27 Da
• Isoelectric Point: 4.9350

>NTDB_id=687518 M3M30_RS08815 WP_274517079.1 1935954..1937024(-) (pilM) [Methylococcus capsulatus strain MIR]
MSAMWGLGRKKPLLLGIDISAAAVKLLELSRKNGGYQVESYGVVPLPRNTVVDNTLAEPDNVSAAVRAVVKQSGTALRHA
VVAVPGSVVITKRIALPANLEGDELEAQIELEADQYIPHAREEVSLDFEVLGKNPKNPDLLDVLLVATRRENVEDRVSVL
ENAGLGVEIVDVESYAIERAFELVRDQLPPLLRDRAVAVADVGAMATTLNVIHGGSIIYTREQGFGGMQLTDEIQRRYGL
TYEEAGLAKKEGSLPGNYAEEVLEPFKHALVQQISRSFQFYLSSTAQRGFDAVVLAGGCAMIPGIDRYVEAALQVPTVIA
NPFRHMSFSGRVRQERLSYDSSALMLACGLALRSFD

Nucleotide

• Length: 1071 bp

>NTDB_id=687518 M3M30_RS08815 WP_274517079.1 1935954..1937024(-) (pilM) [Methylococcus capsulatus strain MIR]
ATGTCAGCCATGTGGGGCCTAGGAAGGAAGAAACCGCTCCTGCTGGGGATCGATATCAGCGCGGCGGCGGTCAAGCTGCT
CGAGCTGAGCAGAAAGAACGGCGGCTACCAGGTGGAAAGCTATGGCGTTGTGCCTTTGCCCCGCAACACGGTGGTCGACA
ACACCCTTGCCGAGCCGGACAACGTGTCGGCGGCGGTCCGGGCCGTGGTCAAACAGTCGGGGACCGCGCTCAGGCACGCG
GTGGTCGCGGTACCCGGCTCGGTGGTCATCACCAAGCGCATCGCCCTACCCGCCAACCTGGAGGGCGACGAACTCGAGGC
GCAGATCGAACTGGAGGCGGACCAGTACATTCCTCACGCCAGGGAGGAAGTCAGCCTGGATTTCGAGGTACTGGGGAAGA
ATCCGAAGAATCCCGATCTGCTGGATGTGCTGCTGGTGGCGACCCGCCGGGAGAACGTGGAAGACCGGGTCTCGGTACTG
GAAAACGCCGGACTCGGCGTCGAGATCGTCGATGTCGAGTCCTATGCCATCGAACGCGCCTTCGAACTTGTCCGGGATCA
GCTTCCACCGCTCCTCCGCGACCGGGCGGTTGCCGTAGCCGATGTGGGCGCCATGGCCACGACGCTCAACGTCATCCATG
GCGGTTCCATCATCTACACGCGGGAACAGGGTTTCGGCGGCATGCAGCTCACCGACGAGATCCAGCGGCGCTACGGTTTG
ACCTACGAGGAAGCGGGGCTGGCGAAGAAGGAGGGGAGCCTTCCCGGCAACTATGCCGAAGAGGTGCTGGAGCCGTTCAA
GCATGCCTTGGTTCAGCAGATCAGCCGATCGTTCCAGTTCTATCTGTCGTCCACTGCGCAGCGTGGTTTCGATGCGGTGG
TGCTGGCCGGCGGCTGCGCCATGATTCCGGGGATAGACCGTTACGTCGAGGCGGCGTTGCAGGTTCCCACCGTGATTGCC
AATCCCTTCCGTCACATGAGTTTTTCGGGAAGGGTGCGGCAGGAGCGCCTGAGTTACGATTCATCCGCCCTGATGCTGGC
ATGCGGCCTGGCACTGCGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (16-355)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	51.603	96.348	0.497
comM	Acinetobacter nosocomialis M2	51.02	96.348	0.492
comM	Acinetobacter baylyi ADP1	49.138	97.753	0.48
pilM	Legionella pneumophila strain ERS1305867	42.733	96.629	0.413