Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   M3L73_RS10445 Genome accession   NZ_CP097102
Coordinates   2161747..2163483 (+) Length   578 a.a.
NCBI ID   WP_249018116.1    Uniprot ID   -
Organism   Vreelandella venusta strain SND-01     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2156747..2168483
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M3L73_RS10415 (M3L73_10415) - 2158060..2158419 (+) 360 WP_146943420.1 hypothetical protein -
  M3L73_RS10420 (M3L73_10420) - 2158498..2159250 (+) 753 WP_125748105.1 VIT1/CCC1 transporter family protein -
  M3L73_RS10430 (M3L73_10430) - 2159600..2160409 (-) 810 WP_125748108.1 inositol monophosphatase family protein -
  M3L73_RS10435 (M3L73_10435) - 2160500..2160955 (-) 456 WP_249018115.1 anti-virulence regulator CigR family protein -
  M3L73_RS21170 - 2161120..2161512 (-) 393 WP_283162148.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  M3L73_RS10445 (M3L73_10445) pilB 2161747..2163483 (+) 1737 WP_249018116.1 type IV-A pilus assembly ATPase PilB Machinery gene
  M3L73_RS10450 (M3L73_10450) - 2163490..2164719 (+) 1230 WP_249018117.1 type II secretion system F family protein -
  M3L73_RS10455 (M3L73_10455) pilD 2164775..2165620 (+) 846 WP_146943533.1 A24 family peptidase Machinery gene
  M3L73_RS10460 (M3L73_10460) coaE 2165661..2166257 (+) 597 WP_249018118.1 dephospho-CoA kinase -
  M3L73_RS10465 (M3L73_10465) yacG 2166297..2166530 (+) 234 WP_083004574.1 DNA gyrase inhibitor YacG -
  M3L73_RS10470 (M3L73_10470) - 2166544..2167416 (+) 873 WP_125748127.1 DNA replication terminus site-binding protein -
  M3L73_RS10475 (M3L73_10475) - 2167448..2167789 (-) 342 WP_125748130.1 hypothetical protein -

Sequence

Protein


Download         Length: 578 a.a.        Molecular weight: 63345.46 Da        Isoelectric Point: 5.5161

>NTDB_id=687043 M3L73_RS10445 WP_249018116.1 2161747..2163483(+) (pilB) [Vreelandella venusta strain SND-01]
MPTLSQTAMHSGLHGIAKRLVENGELTDAQATVAENTATELDVSLLQHLVDSGLVESGAAAQAAAWEFGLPVVELDVIRI
NALPPANDYPVNLLERLEVLPLARHGHRLTVAVPYPATLTQLDELQFATGLSIDGVLAPVDQLKSTLTQYLAQNERSMLD
ELDNVDDAISALNIVQQDEDEATLDKATKESEDAPVVKFVNKILLDAINRGASDIHFEPYETLYRVRFRIDGMLFEIARP
PFALRHRIAARVKIMSRLDISERRLPQDGAIKLQLSRTHSIDFRVNSLPTVFGEKIVLRLLDPATAQLSIEQLGFSSEQQ
AVYEHALKKPQGMILVTGPTGSGKTVTLYTGINILNQIERNICTAEDPVEIKVSGVNQVNVLPKIGLNFASALRAFLRQD
PDVVMVGEIRDLETAEIAVKAAQTGHLVLSTVHTNSAAETLTRLANMGIASYNIASSVSLIIAQRLARKLCSLCKTPAQI
PDEALRQAGFSQQDIRNGTIYSAVGCKHCTQGYKGRIGIYEVVPVTDAMRQLIMRDVDALNIDQQARREGYPSLYQRCLS
RVLDGSTSLAEVNRISKE

Nucleotide


Download         Length: 1737 bp        

>NTDB_id=687043 M3L73_RS10445 WP_249018116.1 2161747..2163483(+) (pilB) [Vreelandella venusta strain SND-01]
ATGCCAACGCTGAGCCAGACAGCCATGCATAGCGGTCTGCACGGTATCGCTAAACGGCTGGTTGAAAATGGCGAGCTGAC
CGATGCTCAGGCGACTGTCGCGGAAAACACAGCAACAGAGCTTGATGTGTCGTTGTTACAGCACCTAGTTGATAGCGGGC
TAGTAGAATCAGGCGCAGCAGCGCAGGCCGCTGCCTGGGAGTTTGGCTTGCCGGTCGTTGAGCTAGATGTCATTCGTATA
AATGCGCTGCCACCCGCCAACGACTATCCCGTTAACTTATTAGAGCGTCTAGAGGTCCTTCCCCTGGCACGCCATGGGCA
TCGACTGACGGTAGCTGTACCCTACCCGGCAACGCTAACCCAGCTAGATGAGCTGCAATTCGCTACCGGGCTAAGTATCG
ATGGAGTATTGGCGCCCGTTGATCAACTTAAAAGCACTCTAACGCAGTATCTAGCGCAAAATGAGCGCAGCATGCTAGAT
GAATTGGACAACGTAGATGATGCGATCAGCGCGTTGAATATTGTTCAGCAGGACGAAGATGAGGCAACCTTAGACAAGGC
AACAAAAGAGAGTGAAGATGCCCCCGTCGTCAAATTCGTCAATAAAATACTCTTAGATGCTATCAACCGCGGCGCTTCCG
ATATTCACTTTGAGCCTTATGAAACCCTGTACCGGGTGCGCTTTCGTATTGATGGCATGCTGTTTGAAATTGCCCGCCCA
CCTTTTGCGCTTCGGCACCGCATTGCGGCTCGGGTAAAAATCATGTCTCGGCTGGACATCTCTGAGCGGCGCCTACCCCA
GGATGGGGCAATCAAGCTGCAGCTTTCACGCACTCACTCAATTGATTTTCGAGTGAACTCGCTGCCCACTGTGTTTGGTG
AAAAAATAGTGCTGCGCTTGCTAGATCCTGCCACTGCTCAACTCTCTATCGAACAACTAGGCTTTAGCTCTGAACAACAG
GCCGTTTATGAACACGCGCTTAAGAAGCCTCAAGGAATGATTCTGGTTACCGGGCCTACGGGAAGCGGTAAAACAGTGAC
GCTTTATACAGGCATCAACATCCTTAACCAAATTGAGCGTAATATTTGCACCGCCGAAGACCCGGTAGAAATTAAAGTGT
CGGGGGTCAATCAGGTCAACGTTCTTCCTAAAATAGGTCTGAACTTCGCTAGTGCTCTACGTGCCTTTTTACGCCAAGAT
CCCGATGTCGTCATGGTGGGCGAAATTCGTGACTTGGAAACCGCCGAAATTGCCGTGAAAGCAGCCCAAACGGGTCACTT
AGTGCTATCTACCGTACACACTAACTCCGCCGCTGAAACACTAACCCGCTTAGCAAATATGGGCATCGCTTCCTATAACA
TCGCCAGCTCGGTTAGCTTGATCATTGCCCAACGCTTGGCACGCAAGCTATGCAGCCTATGCAAAACCCCCGCACAGATT
CCTGACGAAGCACTGCGTCAGGCTGGCTTTAGCCAGCAGGACATTCGCAACGGCACGATTTATTCAGCCGTCGGCTGTAA
ACACTGCACCCAGGGCTATAAAGGTCGTATTGGTATCTATGAAGTGGTGCCCGTTACTGATGCCATGCGCCAGTTGATTA
TGCGTGATGTTGACGCGCTGAATATCGATCAGCAAGCACGCCGCGAAGGTTACCCTAGCCTTTATCAACGCTGCTTAAGT
CGTGTGCTGGATGGCAGCACTAGCTTGGCAGAAGTTAACCGCATCAGTAAGGAGTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(49-155)

Type II/IV secretion system protein (T2SSE)

(202-469)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

51.327

97.751

0.502
  pilB Acinetobacter baumannii D1279779

49.308

100

0.493
  pilB Acinetobacter baylyi ADP1

49.474

98.616

0.488
  pilB Vibrio parahaemolyticus RIMD 2210633

48.427

98.962

0.479
  pilB Vibrio cholerae strain A1552

49.005

95.675

0.469
  pilB Vibrio campbellii strain DS40M4

49.257

93.08

0.458
  pilF Neisseria gonorrhoeae MS11

47.917

83.045

0.398