Detailed information NTG0685756

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: M2J01_RS02045
• Genome accession: NZ_CP096964
• Coordinates: 439050..440198 (+)
• Length: 382 a.a.
• NCBI ID: WP_003110016.1
• Uniprot ID: A0A0H2ZL88
• Organism: Pseudomonas aeruginosa strain NY13936
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 434050..445198

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
M2J01_RS02020 (M2J01_02020)	-	435201..435497 (-)	297	WP_031628073.1	DUF167 domain-containing protein	-
M2J01_RS02025 (M2J01_02025)	-	435494..436087 (-)	594	WP_003084544.1	YggT family protein	-
M2J01_RS02030 (M2J01_02030)	proC	436098..436919 (-)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
M2J01_RS02035 (M2J01_02035)	-	436931..437623 (-)	693	WP_058160881.1	YggS family pyridoxal phosphate-dependent enzyme	-
M2J01_RS02040 (M2J01_02040)	pilT	437838..438872 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
M2J01_RS02045 (M2J01_02045)	pilU	439050..440198 (+)	1149	WP_003110016.1	type IV pilus ATPase PilU	Machinery gene
M2J01_RS02050 (M2J01_02050)	czcD	440206..441105 (-)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
M2J01_RS02055 (M2J01_02055)	-	441260..441664 (+)	405	WP_003084560.1	NINE protein	-
M2J01_RS02060 (M2J01_02060)	-	441908..443281 (+)	1374	WP_003084562.1	pyridoxal-phosphate dependent enzyme	-
M2J01_RS02065 (M2J01_02065)	-	443278..444462 (+)	1185	WP_003110017.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42559.83 Da
• Isoelectric Point: 6.7792

>NTDB_id=685756 M2J01_RS02045 WP_003110016.1 439050..440198(+) (pilU) [Pseudomonas aeruginosa strain NY13936]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSNLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=685756 M2J01_RS02045 WP_003110016.1 439050..440198(+) (pilU) [Pseudomonas aeruginosa strain NY13936]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTAGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAACCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: A0A0H2ZL88

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.22	100	0.882
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382