Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA   Type   Machinery gene
Locus tag   LEUCM_RS09625 Genome accession   NZ_AP017929
Coordinates   1910989..1912326 (-) Length   445 a.a.
NCBI ID   WP_052039239.1    Uniprot ID   A0AAE8J6D0
Organism   Latilactobacillus sakei subsp. sakei DSM 20017 = JCM 1157 strain LT-13     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1905989..1917326
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LEUCM_RS09610 (LACBS_01912) secA 1907034..1909397 (-) 2364 WP_011374203.1 preprotein translocase subunit SecA -
  LEUCM_RS09615 (LACBS_01913) hpf 1909646..1910191 (-) 546 WP_011374202.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  LEUCM_RS09880 (LACBS_01914) comFC 1910318..1910746 (-) 429 WP_151384770.1 ComF family protein Machinery gene
  LEUCM_RS09625 (LACBS_01915) comFA 1910989..1912326 (-) 1338 WP_052039239.1 DEAD/DEAH box helicase Machinery gene
  LEUCM_RS09630 (LACBS_01916) - 1912388..1913053 (+) 666 WP_025016329.1 YigZ family protein -
  LEUCM_RS09635 (LACBS_01917) - 1913080..1914168 (-) 1089 WP_025016330.1 glycosyltransferase family 4 protein -
  LEUCM_RS09640 (LACBS_01918) rny 1914324..1915889 (-) 1566 WP_025016331.1 ribonuclease Y -

Sequence

Protein


Download         Length: 445 a.a.        Molecular weight: 50353.78 Da        Isoelectric Point: 9.7862

>NTDB_id=68573 LEUCM_RS09625 WP_052039239.1 1910989..1912326(-) (comFA) [Latilactobacillus sakei subsp. sakei DSM 20017 = JCM 1157 strain LT-13]
MGQQVIACGRQFTAAQLADTQNNNYSLPQIKRRPAFLRVKHRLVCQRCQQVVPPQTCLPDGRHYCAQCLLFGRLVEGDWL
YTVPECHLFENNIPKLTWEGQLTPHQEQAAQAVVEVVQTQKRHVLTAVTGAGKTEMLFQGILVALQKGQRVCLAAPRVAV
CLELYPRLQAAFATASIMLMHGEQTEPYRYTQLVICTTHQLLKFYHAFDTVIVDEVDAFPFVDNPVLVTAVEQACKPQCA
LLYLTATPTPAIKRAIAAKQMTVSELPLRFHGHLLPEPHRHVAFNWRAQLKKGRLPKRLERDCQTCLKTQQILLFVPQVR
LLQPVANRLSTLLPAVRIETLHANDPDQIKKITAFRAQQIQLMVTTTILERGVTFKNVAVLVLGAEHMVFNEAVLVQIAG
RAGRHKDYAQNPVHFYYQDYTRAIKGACRQIKAQNQRGRRLQSTV

Nucleotide


Download         Length: 1338 bp        

>NTDB_id=68573 LEUCM_RS09625 WP_052039239.1 1910989..1912326(-) (comFA) [Latilactobacillus sakei subsp. sakei DSM 20017 = JCM 1157 strain LT-13]
ATGGGACAACAAGTAATTGCCTGTGGTCGCCAGTTTACGGCGGCCCAGTTAGCCGATACGCAAAATAATAACTATAGTTT
ACCACAGATTAAGCGGCGACCTGCGTTTTTACGGGTCAAGCATCGCCTAGTCTGCCAACGTTGTCAGCAAGTGGTGCCGC
CGCAAACCTGCTTACCAGATGGGCGCCATTATTGCGCGCAATGTTTGTTATTTGGTCGTTTGGTAGAGGGCGATTGGTTA
TACACGGTACCGGAGTGTCATTTATTCGAAAATAACATACCAAAGTTGACGTGGGAGGGGCAATTAACACCGCATCAGGA
ACAAGCTGCCCAAGCGGTGGTTGAGGTGGTTCAAACACAAAAACGGCACGTATTAACGGCCGTGACCGGCGCAGGGAAGA
CCGAGATGCTTTTTCAAGGAATTTTAGTTGCGCTTCAAAAGGGTCAGCGGGTATGTCTGGCAGCGCCTAGAGTCGCCGTT
TGCTTGGAACTTTATCCGCGCCTGCAAGCTGCCTTTGCAACGGCTAGTATCATGTTGATGCACGGTGAGCAAACTGAGCC
CTATCGCTATACGCAGTTAGTGATTTGCACGACGCATCAGTTGCTGAAGTTTTATCACGCCTTTGACACTGTGATTGTCG
ATGAAGTCGATGCTTTTCCTTTTGTTGATAATCCCGTTTTAGTCACGGCAGTTGAACAAGCCTGCAAGCCCCAATGTGCT
TTATTATATTTAACGGCGACTCCGACCCCCGCTATTAAACGGGCAATTGCTGCTAAACAAATGACGGTTAGCGAATTACC
GCTCCGCTTTCATGGTCATCTATTACCAGAACCACATAGGCACGTCGCTTTTAATTGGCGGGCCCAATTAAAAAAAGGTC
GGTTACCTAAACGACTTGAGCGGGATTGCCAGACTTGTTTAAAAACACAACAGATTTTGTTATTCGTGCCGCAAGTGCGG
TTATTGCAACCAGTTGCCAATCGGTTATCAACCTTATTGCCGGCGGTCAGGATTGAAACATTGCATGCTAACGATCCGGA
TCAGATCAAAAAGATTACGGCGTTTAGAGCACAACAAATTCAGCTAATGGTGACGACCACCATTTTAGAACGGGGCGTTA
CGTTTAAAAACGTTGCAGTTTTGGTCTTAGGCGCGGAACACATGGTGTTTAACGAAGCCGTTTTGGTCCAGATTGCCGGG
CGAGCGGGACGGCATAAAGATTACGCCCAAAATCCGGTTCATTTTTATTATCAAGACTATACGCGGGCGATCAAAGGCGC
TTGTCGGCAAATTAAGGCCCAAAATCAGAGGGGACGGCGATTACAATCAACTGTTTAA

Domains

Predicted by InterproScan.

Helicase conserved C-terminal domain (Helicase_C)

(307-405)

Type III restriction enzyme, res subunit (ResIII)

(101-248)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA Latilactobacillus sakei subsp. sakei 23K

98.652

100

0.987
  comFA/cflA Streptococcus pneumoniae TIGR4

38.979

96.854

0.378
  comFA/cflA Streptococcus pneumoniae D39

38.747

96.854

0.375
  comFA/cflA Streptococcus pneumoniae R6

38.747

96.854

0.375
  comFA/cflA Streptococcus pneumoniae Rx1

38.747

96.854

0.375
  comFA/cflA Streptococcus mitis SK321

38.515

96.854

0.373
  comFA/cflA Streptococcus mitis NCTC 12261

38.283

96.854

0.371
  comFA Bacillus subtilis subsp. subtilis str. 168

39.268

92.135

0.362

Multiple sequence alignment