Detailed information NTG0685481

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: M2I92_RS02405
• Genome accession: NZ_CP096945
• Coordinates: 488057..489205 (+)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain NY5525
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 483057..494205

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
M2I92_RS02380 (M2I92_02380)	-	484208..484504 (-)	297	WP_031628111.1	DUF167 domain-containing protein	-
M2I92_RS02385 (M2I92_02385)	-	484501..485094 (-)	594	WP_003084544.1	YggT family protein	-
M2I92_RS02390 (M2I92_02390)	proC	485105..485926 (-)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
M2I92_RS02395 (M2I92_02395)	-	485938..486630 (-)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
M2I92_RS02400 (M2I92_02400)	pilT	486845..487879 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
M2I92_RS02405 (M2I92_02405)	pilU	488057..489205 (+)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
M2I92_RS02410 (M2I92_02410)	czcD	489213..490112 (-)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
M2I92_RS02415 (M2I92_02415)	-	490266..490670 (+)	405	WP_003084560.1	NINE protein	-
M2I92_RS02420 (M2I92_02420)	-	490913..492286 (+)	1374	WP_034039850.1	pyridoxal-phosphate dependent enzyme	-
M2I92_RS02425 (M2I92_02425)	-	492283..493467 (+)	1185	WP_003110017.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=685481 M2I92_RS02405 WP_003100959.1 488057..489205(+) (pilU) [Pseudomonas aeruginosa strain NY5525]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=685481 M2I92_RS02405 WP_003100959.1 488057..489205(+) (pilU) [Pseudomonas aeruginosa strain NY5525]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382