Detailed information NTG0685382

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: M2I89_RS02200
• Genome accession: NZ_CP096937
• Coordinates: 477964..479112 (+)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain NY5520
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 472964..484112

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
M2I89_RS02175 (M2I89_02175)	-	474115..474411 (-)	297	WP_031628111.1	DUF167 domain-containing protein	-
M2I89_RS02180 (M2I89_02180)	-	474408..475001 (-)	594	WP_003084544.1	YggT family protein	-
M2I89_RS02185 (M2I89_02185)	proC	475012..475833 (-)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
M2I89_RS02190 (M2I89_02190)	-	475845..476537 (-)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
M2I89_RS02195 (M2I89_02195)	pilT	476752..477786 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
M2I89_RS02200 (M2I89_02200)	pilU	477964..479112 (+)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
M2I89_RS02205 (M2I89_02205)	czcD	479120..480019 (-)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
M2I89_RS02210 (M2I89_02210)	-	480174..480578 (+)	405	WP_003084560.1	NINE protein	-
M2I89_RS02215 (M2I89_02215)	-	480821..482194 (+)	1374	WP_003100955.1	pyridoxal-phosphate dependent enzyme	-
M2I89_RS02220 (M2I89_02220)	-	482191..483375 (+)	1185	WP_003110017.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=685382 M2I89_RS02200 WP_003100959.1 477964..479112(+) (pilU) [Pseudomonas aeruginosa strain NY5520]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=685382 M2I89_RS02200 WP_003100959.1 477964..479112(+) (pilU) [Pseudomonas aeruginosa strain NY5520]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGTCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382