Detailed information NTG0068536

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: sS8_RS25735
• Genome accession: NZ_AP017928
• Coordinates: 5726825..5727886 (+)
• Length: 353 a.a.
• NCBI ID: WP_119632259.1
• Uniprot ID: A0A250L047
• Organism: Methylocaldum marinum strain S8
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 5721825..5732886

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
sS8_RS25715 (sS8_5312)	-	5722207..5722986 (-)	780	WP_119632255.1	exodeoxyribonuclease III	-
sS8_RS25720 (sS8_5314)	rpmE	5723194..5723397 (+)	204	WP_119632256.1	50S ribosomal protein L31	-
sS8_RS25725 (sS8_5315)	-	5723564..5724154 (-)	591	WP_119632257.1	hypothetical protein	-
sS8_RS25730 (sS8_5316)	-	5724163..5726574 (-)	2412	WP_119632258.1	penicillin-binding protein 1A	-
sS8_RS25735 (sS8_5317)	pilM	5726825..5727886 (+)	1062	WP_119632259.1	pilus assembly protein PilM	Machinery gene
sS8_RS25740 (sS8_5318)	-	5727886..5728470 (+)	585	WP_119632260.1	PilN domain-containing protein	-
sS8_RS25745 (sS8_5319)	-	5728467..5729114 (+)	648	WP_119632261.1	type 4a pilus biogenesis protein PilO	-
sS8_RS25750 (sS8_5320)	-	5729218..5729685 (+)	468	WP_232020443.1	pilus assembly protein PilP	-
sS8_RS25755 (sS8_5321)	pilQ	5729682..5731916 (+)	2235	WP_119632263.1	type IV pilus secretin PilQ	-
sS8_RS25760 (sS8_5322)	aroK	5732048..5732578 (+)	531	WP_119633019.1	shikimate kinase AroK	-

Sequence

Protein

• Length: 353 a.a.
• Molecular weight: 38363.06 Da
• Isoelectric Point: 5.1175

>NTDB_id=68536 sS8_RS25735 WP_119632259.1 5726825..5727886(+) (pilM) [Methylocaldum marinum strain S8]
MFFLNRKRPQLLGIDISTAAVKLLELSKNGQRYRVESYAVAPLPLDAVIDKNIAKVEIIGSVIKSVIKQAGTRLKHAAVA
VPSSSVITKMISMPASLNEDDLEAQIELEADQYIPYSLDEVSLDFEVQGRAVKNPDMVDVLLVASRKENVEDRVAALEMA
GLKAEIVDVESYALENACNLILDQLPGYSPDQTIAVADVGSTMTALNVMHDRRIIYTREQGFGGKQLTEEIQRRYGLSYE
EAGLAKKHGGLPDNYLLEVLEPFKQAMAQQIGRSLQFFLSSSSHRTVDGLVLAGGCGSIHGIDRFVEGVLGIPTTAANPF
GHMSLAPRIKPQSLNNDAPALMTACGLALRSLD

Nucleotide

• Length: 1062 bp

>NTDB_id=68536 sS8_RS25735 WP_119632259.1 5726825..5727886(+) (pilM) [Methylocaldum marinum strain S8]
ATGTTTTTTTTGAACCGCAAGAGACCGCAGTTGCTTGGCATTGATATCAGCACGGCGGCCGTCAAGCTGCTGGAGCTGAG
CAAAAACGGCCAGCGCTACCGGGTCGAAAGCTACGCCGTCGCGCCGTTGCCATTGGACGCCGTGATCGACAAGAACATCG
CAAAGGTCGAAATCATCGGCAGCGTAATCAAATCCGTCATCAAGCAAGCCGGGACCCGGTTGAAGCATGCCGCGGTAGCG
GTGCCGAGTTCATCGGTAATCACCAAGATGATCTCGATGCCGGCTTCCTTGAACGAAGACGACTTGGAGGCCCAGATTGA
ACTGGAAGCCGACCAATATATTCCATACTCGCTGGATGAAGTGAGCCTGGATTTCGAAGTGCAGGGCAGAGCCGTGAAGA
ATCCGGACATGGTTGACGTGCTGCTGGTCGCCTCGCGGAAAGAGAATGTGGAAGATCGCGTGGCGGCTCTGGAAATGGCC
GGGCTCAAGGCCGAGATTGTCGATGTGGAATCGTATGCTCTGGAGAATGCCTGCAACTTAATTCTGGACCAGCTGCCGGG
ATACAGTCCCGATCAAACCATCGCAGTGGCCGATGTCGGATCGACCATGACCGCCCTGAACGTAATGCATGACCGCCGAA
TCATCTATACGCGCGAACAGGGATTCGGCGGCAAGCAGTTGACCGAAGAAATCCAGCGCCGGTACGGGCTTTCTTATGAA
GAGGCCGGCTTGGCCAAAAAACACGGCGGTCTTCCGGACAATTATCTGCTCGAGGTTCTGGAGCCGTTCAAACAGGCAAT
GGCGCAGCAGATCGGGCGTTCACTCCAATTCTTTCTTTCCTCCAGCTCGCATCGGACTGTGGATGGGCTGGTGTTGGCAG
GAGGCTGCGGCTCTATCCACGGTATAGACCGCTTCGTCGAAGGGGTTCTCGGAATCCCCACCACCGCAGCCAACCCGTTT
GGACACATGTCTCTCGCGCCACGGATTAAACCACAGAGTTTGAATAATGACGCCCCGGCATTGATGACTGCATGCGGACT
GGCTTTAAGGAGCTTGGACTAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (13-351)

3D structure

• AlphaFold DB: A0A250L047

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	51.695	100	0.518
comM	Acinetobacter nosocomialis M2	51.412	100	0.516
comM	Acinetobacter baylyi ADP1	49.143	99.15	0.487
pilM	Legionella pneumophila strain ERS1305867	43.02	99.433	0.428