Detailed information NTG0068532

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: sS8_RS18325
• Genome accession: NZ_AP017928
• Coordinates: 4102968..4104194 (-)
• Length: 408 a.a.
• NCBI ID: WP_119631026.1
• Uniprot ID: A0A250KVT8
• Organism: Methylocaldum marinum strain S8
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4097968..4109194

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
sS8_RS18300 (sS8_3785)	metK	4098256..4099425 (-)	1170	WP_119631021.1	methionine adenosyltransferase	-
sS8_RS18305 (sS8_3786)	-	4099545..4100030 (-)	486	WP_145986597.1	hypothetical protein	-
sS8_RS18310 (sS8_3787)	ppa	4100197..4100745 (+)	549	WP_119631023.1	inorganic diphosphatase	-
sS8_RS18315 (sS8_3788)	-	4100787..4101629 (+)	843	WP_119631024.1	23S rRNA (adenine(2030)-N(6))-methyltransferase RlmJ	-
sS8_RS18320 (sS8_3789)	-	4101897..4102595 (-)	699	WP_145986598.1	hypothetical protein	-
sS8_RS18325 (sS8_3790)	pilU	4102968..4104194 (-)	1227	WP_119631026.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
sS8_RS18330 (sS8_3791)	-	4104710..4104946 (+)	237	WP_145986599.1	hypothetical protein	-
sS8_RS18335 (sS8_3792)	-	4105020..4109156 (-)	4137	WP_119631028.1	chemotaxis protein CheB	-

Sequence

Protein

• Length: 408 a.a.
• Molecular weight: 45474.95 Da
• Isoelectric Point: 6.5114

>NTDB_id=68532 sS8_RS18325 WP_119631026.1 4102968..4104194(-) (pilU) [Methylocaldum marinum strain S8]
MKFTSYLKLLVYKDGSDLYLTAGAPPAAKFQGKLTPIDTAEMTDEMIKEIAYSVMDEGQRRDFERRPEMNLALSEEGVGR
FRVNIFKQRNAHAMVVRNIKVDIPKVDDLGLPEVLKKVIMEKRGLVLFVGATGSGKSTSLAALIDHRNSNSAGHIVTIED
PIEFLHPHKKCLVNQREVGVDTQTYEDALKNTLRQAPDVILIGEIRSQDTMEYAINFAETGHLCLSTLHANNANQALDRI
INFFPEERRNQLLLDLSLNLRAFVSQRLVPTVDGKRTAAIEIMLGTPLVCDFIRKGEIHAIKEVMEKSEGLGMQTFDSHL
VKLYQAGRVTAEEAIRNADSANNVRLKISLSQGKRRTPGEASPESAKTSRNQLREKAAKAQADESAFAMMNLSLEPVGDE
NEATEEFH

Nucleotide

• Length: 1227 bp

>NTDB_id=68532 sS8_RS18325 WP_119631026.1 4102968..4104194(-) (pilU) [Methylocaldum marinum strain S8]
ATGAAATTTACGAGTTACCTCAAGCTTCTGGTCTACAAGGACGGGTCTGATCTTTATCTCACGGCCGGCGCCCCGCCCGC
CGCCAAATTTCAAGGGAAGCTCACACCGATCGATACCGCCGAAATGACCGACGAAATGATCAAGGAAATCGCCTATTCGG
TGATGGATGAGGGCCAGCGGCGGGATTTCGAGCGGCGGCCGGAAATGAATCTCGCCCTAAGCGAGGAAGGCGTGGGACGT
TTTCGGGTGAACATCTTTAAACAGCGCAATGCCCATGCGATGGTGGTGCGAAACATCAAAGTCGACATTCCCAAAGTGGA
CGATCTCGGTCTGCCGGAAGTCCTCAAGAAAGTCATCATGGAAAAGCGGGGGCTAGTCCTGTTCGTGGGCGCCACCGGTT
CCGGCAAATCGACTTCGCTGGCGGCACTGATCGACCATCGCAACAGCAATTCCGCCGGGCACATCGTCACTATCGAAGAT
CCGATCGAGTTCCTGCACCCCCACAAGAAGTGCCTGGTGAATCAGCGCGAGGTGGGTGTCGATACCCAGACCTACGAGGA
CGCCCTCAAGAACACGCTTCGGCAGGCACCCGACGTCATCCTGATCGGCGAAATCCGCTCCCAGGACACCATGGAATACG
CCATCAATTTCGCGGAAACCGGCCATTTGTGTCTATCGACCCTGCACGCCAACAACGCGAACCAGGCGCTGGACCGCATC
ATCAATTTCTTCCCTGAAGAACGGCGCAATCAGCTTCTGCTGGATTTGTCCCTCAACCTGCGCGCTTTCGTCTCGCAGCG
CCTGGTGCCGACGGTGGACGGCAAGCGAACCGCGGCCATCGAGATCATGCTCGGAACCCCTTTGGTCTGCGACTTCATCC
GCAAGGGCGAAATCCACGCGATCAAAGAAGTCATGGAGAAATCGGAAGGTCTTGGCATGCAGACTTTCGACAGTCATTTG
GTGAAGCTGTACCAAGCCGGCCGCGTTACCGCGGAAGAAGCGATCCGAAACGCGGACTCGGCCAACAACGTCCGCTTGAA
AATCAGCCTGAGCCAGGGCAAACGACGTACCCCCGGAGAGGCGTCGCCGGAAAGTGCCAAAACTTCCCGGAACCAGCTTC
GAGAAAAGGCCGCGAAAGCTCAAGCCGACGAATCGGCATTCGCCATGATGAATCTGAGCCTGGAACCGGTCGGCGACGAA
AACGAAGCGACCGAGGAATTCCACTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (38-270)

3D structure

• AlphaFold DB: A0A250KVT8

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	60.399	86.029	0.52
pilU	Acinetobacter baylyi ADP1	53.39	86.765	0.463
pilU	Vibrio cholerae strain A1552	48.87	86.765	0.424
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	41.408	87.01	0.36