Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   LFL98_RS13035 Genome accession   NZ_CP110365
Coordinates   2511647..2512057 (-) Length   136 a.a.
NCBI ID   WP_009967785.1    Uniprot ID   P42983
Organism   Bacillus subtilis strain HY2-62     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2506647..2517057
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LFL98_RS13005 (LFL98_13010) - 2506734..2506874 (+) 141 WP_003226124.1 sporulation histidine kinase inhibitor Sda -
  LFL98_RS13010 (LFL98_13015) yqeF 2507180..2507911 (-) 732 WP_003229964.1 SGNH/GDSL hydrolase family protein -
  LFL98_RS13015 (LFL98_13020) cwlH 2508163..2508915 (-) 753 WP_080529562.1 N-acetylmuramoyl-L-alanine amidase CwlH -
  LFL98_RS13020 (LFL98_13025) yqeD 2509102..2509728 (+) 627 WP_213420161.1 TVP38/TMEM64 family protein -
  LFL98_RS13025 (LFL98_13030) gnd 2509747..2510640 (-) 894 WP_015714298.1 phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating) -
  LFL98_RS13030 (LFL98_13035) yqeB 2510892..2511614 (+) 723 WP_042976542.1 hypothetical protein -
  LFL98_RS13035 (LFL98_13040) nucA/comI 2511647..2512057 (-) 411 WP_009967785.1 sporulation-specific Dnase NucB Machinery gene
  LFL98_RS13040 (LFL98_13045) - 2512253..2512597 (+) 345 Protein_2519 sigma-70 family RNA polymerase sigma factor -
  LFL98_RS13045 (LFL98_13050) - 2512743..2513213 (+) 471 WP_015251668.1 MarR family transcriptional regulator -
  LFL98_RS13050 (LFL98_13055) - 2513364..2514776 (+) 1413 WP_277736543.1 MDR family MFS transporter -
  LFL98_RS13055 (LFL98_13060) spoIVCA 2514963..2516423 (-) 1461 WP_277737219.1 site-specific DNA recombinase SpoIVCA -
  LFL98_RS13060 (LFL98_13065) - 2516381..2516559 (-) 179 Protein_2523 hypothetical protein -

Sequence

Protein


Download         Length: 136 a.a.        Molecular weight: 14967.97 Da        Isoelectric Point: 5.1853

>NTDB_id=684735 LFL98_RS13035 WP_009967785.1 2511647..2512057(-) (nucA/comI) [Bacillus subtilis strain HY2-62]
MKKWMAGLFLAAAVLLCLMVPQQIQGASSYDKVLYFPLSRYPETGSHIRDAIAEGHPDICTIDRDGADKRREESLKGIPT
KPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSSYPDGTRVLFIVQ

Nucleotide


Download         Length: 411 bp        

>NTDB_id=684735 LFL98_RS13035 WP_009967785.1 2511647..2512057(-) (nucA/comI) [Bacillus subtilis strain HY2-62]
ATGAAAAAATGGATGGCAGGCCTGTTTCTTGCTGCAGCAGTTCTTCTTTGTTTAATGGTTCCGCAACAGATCCAAGGCGC
ATCTTCGTATGACAAAGTGTTATATTTTCCGCTGTCTCGTTATCCGGAAACCGGCAGTCATATTAGGGATGCGATTGCAG
AGGGACATCCAGATATTTGTACCATTGACAGAGATGGAGCAGACAAAAGGCGGGAGGAATCTTTAAAGGGAATCCCGACC
AAGCCGGGCTATGACCGGGATGAGTGGCCGATGGCGGTCTGCGAGGAAGGCGGTGCAGGGGCTGATGTCCGATATGTGAC
GCCTTCTGATAATCGCGGCGCCGGCTCGTGGGTAGGGAATCAAATGAGCAGCTACCCTGACGGCACCAGAGTGCTGTTTA
TTGTGCAGTAA

Domains

Predicted by InterProScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(40-130)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P42983

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

62.609

84.559

0.529